Effect of GABA_B receptor blockade on receptive fields of raccoon somatosensory cortical neurons during reorganization

Abstract

Single unit recordings were made in the fourth digit representation of raccoon somatosensory cortex after amputation of the fourth digit. The receptive fields of neurons in this “reorganized” cortex were studied before and after microiontophoretic application of a highly specific GABA_B receptor antagonist, CGP 55845. When the recordings were performed early in the reorganization process, 3–5 weeks after amputation, CGP 55845 produced a greater expansion of the receptive field (184%) than at longer postamputation intervals (17–34 weeks, 137% increase) or previously reported in intact animals (105%). Most of the receptive fields in these animals were restricted to either an adjacent digit or the palm and the expansion was adjacent to the predrug receptive field. However, in some cases block of GABA_B receptors unmasked new responsive areas that were separated from the predrug field. In addition, GABA_B receptor block often revealed higher threshold fields on an adjacent digit or palm. These results reinforce previous studies that used a GABA_A receptor antagonist, and together they reveal the importance of GABAergic synapses in regulating the various inputs to cortical somatosensory neurons during reorganization.