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A highly sensitive europium nanoparticle-based lateral flow immunoassay for detection of chloramphenicol residue

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Abstract

A europium nanoparticle-based lateral flow immunoassay for highly sensitive detection of chloramphenicol residue was developed. The detection result could be either qualitatively resolved with naked eye or quantitatively analyzed with the assistance of a digital camera. In the qualitative mode, the limit of detection (LOD) was found to be 0.25 ng/mL. In the quantitative mode, the half-maximal inhibition concentration (IC50) was determined to be 0.45 ng/mL and the LOD can reach an ultralow level of 0.03 ng/mL, which is ~100 times lower than that of the conventional colloidal gold-based lateral flow immunoassay. Potential application of the established method was demonstrated by analyzing representative cow milk samples.

A europium nanoparticle-based lateral flow immunoassay for highly sensitive detection of chloramphenicol (CAP) residue was developed, of which limit of detections (LODs) can reach low levels of 0.25 and 0.03 ng/mL, respectively, in the qualitative and quantitative modes.

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Acknowledgments

The authors acknowledge financial support for this work by the Commonweal Scientific Foundation for Industry of Chinese Inspection and Quarantine (no. 201010022), the Xiamen Scientific Development Program (no. 3502Z20055008), the National Natural Science Foundation of China (no. 30500454), and the Natural Science Foundation of Fujian Province of China (no. 2007 J0112).

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Author notes

  1. Xiaohu Xia

    Present address: The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University School of Medicine, Georgia Institute of Technology, Atlanta, GA, 30332, USA

Authors and Affiliations

  1. Engineering Research Centre of Molecular Diagnostics, Ministry of Education; Department of Biomedical Sciences, School of Life Sciences, Xiamen University, Xiamen, Fujian, 361005, China

    Xiaohu Xia, Ye Xu, Rongqin Ke, Wei Yang & Qingge Li

  2. Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, 518067, China

    Heng Zhang

  3. Chinese Academy of Inspection and Quarantine, Beijing, 100123, China

    Mingqiang Zou

Authors
  1. Xiaohu Xia
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  2. Ye Xu
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  4. Heng Zhang
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  7. Qingge Li
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Corresponding author

Correspondence to Qingge Li.

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Xiaohu Xia and Ye Xu contributed equally to this work.

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Xia, X., Xu, Y., Ke, R. et al. A highly sensitive europium nanoparticle-based lateral flow immunoassay for detection of chloramphenicol residue. Anal Bioanal Chem 405, 7541–7544 (2013). https://doi.org/10.1007/s00216-013-7210-9

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  • DOI: https://doi.org/10.1007/s00216-013-7210-9

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