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Toward the quantification of the 13CO2/12CO2 ratio in exhaled mouse breath with mid-infrared hollow waveguide gas sensors

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Abstract

Mouse sepsis models are used to gain insight into the complex processes involved with patients suffering from glucose metabolism disorders. Measuring the expiratory release of 13CO2 after administering stable labeled 13C6-glucose enables assessment of the in vivo integrity and functionality of key metabolic processes. In the present study, we demonstrate that Fourier transform infrared spectroscopy operating in the mid-infrared spectral regime (2–20 μm) combined with hollow waveguide gas sensing modules simultaneously serving as a miniaturized gas cell and as a waveguide are capable of quantitatively monitoring 13CO2 enrichment levels in low volume mouse breath samples.

Mouse intensive care unit (MICU) with patient and IR signature of the time progression for 12CO2/13CO2 in exhaled mouse breath

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Notes

  1. The δ13C value in ‰ is defined as

    [21].

Abbreviations

CLS:

Classical least squares

FTIR:

Fourier transform infrared

GC/MS:

Gas chromatography/mass spectrometry

HWG:

Hollow waveguide

IRMS:

Isotope ratio mass spectrometry

LARA:

Laser-assisted ratio analysis

MCT:

Mercury–cadmium–telluride

MICU:

Mouse intensive care unit

MIR:

Mid-infrared

MS:

Mass spectrometry

NIR:

Near-infrared

NDIRS:

Non-dispersive isotope selective infrared spectroscopy

NMR:

Nuclear magnetic resonance

OAPM:

Off-axis parabolic mirror

PLS:

Partial least squares

QCL:

Quantum cascade laser

SIM:

Selected-ion-monitoring

S/N :

Signal-to-noise ratio

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Acknowledgments

The authors acknowledge the support of the team operating the mouse intensive care unit (MICU) at the Klinik für Anästhesiologie, Sektion Anästhesiologische Pathophysiologie und Verfahrensentwicklung, Universitätsklinikum Ulm, and especially Sandra Weber for assistance during the mouse breath studies.

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Authors and Affiliations

  1. Institute of Analytical and Bioanalytical Chemistry, University of Ulm, 89081, Ulm, Germany

    Andreas Wilk, Felicia Seichter, Seong-Soo Kim, Erhan Tütüncü & Boris Mizaikoff

  2. Klinik für Anästhesiologie, Sektion Anästhesiologische Pathophysiologie und Verfahrensentwicklung, Universitätsklinikum Ulm, 89073, Ulm, Germany

    Josef A. Vogt, Ulrich Wachter & Peter Radermacher

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  1. Andreas Wilk
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  2. Felicia Seichter
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  3. Seong-Soo Kim
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  4. Erhan Tütüncü
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  5. Boris Mizaikoff
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Correspondence to Boris Mizaikoff.

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Published in the 10th Anniversary Issue.

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Wilk, A., Seichter, F., Kim, SS. et al. Toward the quantification of the 13CO2/12CO2 ratio in exhaled mouse breath with mid-infrared hollow waveguide gas sensors. Anal Bioanal Chem 402, 397–404 (2012). https://doi.org/10.1007/s00216-011-5524-z

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  • DOI: https://doi.org/10.1007/s00216-011-5524-z

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