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Structural and biological evaluation of a multifunctional SWCNT-AgNPs-DNA/PVA bio-nanofilm

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Abstract

A bio-nanofilm consisting of a tetrad nanomaterial (nanotubes, nanoparticles, DNA, polymer) was fabricated utilizing in situ reduction and noncovalent interactions and it displayed effective antibacterial activity and biocompatibility. This bio-nanofilm was composed of homogenous silver nanoparticles (AgNPs) coated on single-walled carbon nanotubes (SWCNTs), which were later hybridized with DNA and stabilized in poly(vinyl alcohol) (PVA) in the presence of a surfactant with the aid of ultrasonication. Electron microscopy and bio-AFM (atomic force microscopy) images were used to assess the morphology of the nanocomposite (NC) structure. Functionalization and fabrication were examined using FT–Raman spectroscopy by analyzing the functional changes in the bio-nanofilm before and after fabrication. UV–visible spectroscopy and X-ray powder diffraction (XRD) confirmed that AgNPs were present in the final NC on the basis of its surface plasmon resonance (370 nm) and crystal planes. Thermal gravimetric analysis was used to measure the percentage weight loss of SWCNT (17.5%) and final SWCNT-AgNPs-DNA/PVA (47.7%). The antimicrobial efficiency of the bio-nanofilm was evaluated against major pathogenic organisms. Bactericidal ratios, zone of inhibition, and minimum inhibitory concentration were examined against gram positive and gram negative bacteria. A preliminary cytotoxicity analysis was conducted using A549 lung cancer cells and IMR-90 fibroblast cells. Confocal laser microscopy, bio-AFM, and field emission scanning electron microscopy (FE-SEM) images demonstrated that the NCs were successfully taken up by the cells. These combined results indicate that this bio-nanofilm was biocompatible and displayed antimicrobial activity. Thus, this novel bio-nanofilm holds great promise for use as a multifunctional tool in burn therapy, tissue engineering, and other biomedical applications.

Schematic representation of the fabrication of bio-nanofilm, and it's photographic images and applications.

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Acknowledgements

This work was supported by Kyungwon University research fund in 2010 and Grant No. 10032112 from the Regional Technology Innovation Program of the Ministry of Knowledge Economy. This study was supported by a grant of the Ministry of Health & Welfare (A040041) and Samsung Biomedical Research Institute, Republic of Korea (PB00021).

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Authors and Affiliations

  1. College of Bionanotechnology, Kyungwon University, Gyeonggi-do, 461-701, South Korea

    Ramesh P. Subbiah, Murugan Veerapandian, Sathya Sadhasivam & Kyusik Yun

  2. Interdisciplinary Graduate Program of Biomedical Engineering, School of Medicine, Sungkyunkwan University, 50 Irwon-Dong, Gangnam-Gu, Seoul, 135-710, Korea

    Haisung Lee & Soo-won Seo

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  1. Ramesh P. Subbiah
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  2. Haisung Lee
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Correspondence to Kyusik Yun.

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Subbiah, R.P., Lee, H., Veerapandian, M. et al. Structural and biological evaluation of a multifunctional SWCNT-AgNPs-DNA/PVA bio-nanofilm. Anal Bioanal Chem 400, 547–560 (2011). https://doi.org/10.1007/s00216-011-4757-1

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