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Development of a micro-total analysis system (μ-TAS) for the determination of catecholamines

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Abstract

In this study, the first micro-total analysis system (μ-TAS) for catecholamines (dopamine, epinephrine, and norepinephrine) analysis in which preconcentration, separation, and determination steps were integrated on a microchip was developed. Electrophoresis microchips in a variety of channel lengths and designs were produced in borofloat glass for the μ-TAS studies. Chambers for the preparation of monolithic disks were formed in the microchips at the intersection of the injection and separation channels. Vinyl phenylboronic acid–ethylene glycol dimethacrylate polymers were prepared as monolithic disks in these chambers with a depth of 0.05 mm and a diameter of 2.1 mm. The microchips could be used more than 50 times if mechanical problems such as plugging or fracturing did not occur. Adsorption and elution of catecholamines were realized electrokinetically, with catecholamines determined via laser-induced native fluorescence detection following elution and electrophoretic separation. The most promising results were obtained with 100 mM phosphate buffer (pH 2) for elution with 25% propanol added to the separation buffer (100 mM phosphate, pH 3).

The boronic acid functional affinity monolith containing microchip

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References

  1. Wen J, Guillo C, Ferrance JP, Landers JP (2007) J Chromatogr A 1171:29–36

    Article  CAS  Google Scholar 

  2. Schulze P, Schuttpelz M, Sauer M, Belder D (2007) Lab Chip 7:1841–1844

    Article  CAS  Google Scholar 

  3. Li HF, Cai ZW, Lin JM (2006) Anal Chim Acta 565:183–189

    Article  CAS  Google Scholar 

  4. Dawoud AA, Kawaguchi T, Markushin Y, Porter MD, Jankowiak R (2006) Sens Actuators B 120:42–50

    Article  Google Scholar 

  5. Vlckova M, Schwarz MA (2007) J Chromatogr A 1142:214–221

    Article  CAS  Google Scholar 

  6. Manz A, Graber N, Widmer HM (1990) Sens Actuators B 1:244–248

    Article  Google Scholar 

  7. Gac SL, Carlier J, Camart JC, Olive CC, Rolando C (2004) J Chromatogr B 808:3–14

    Article  Google Scholar 

  8. Cao Li-Wei (2007) Biomed Chromatogr 21:708–715

    Article  CAS  Google Scholar 

  9. Britz-McKibbin P, Chen DDY (2000) Anal Chem 72:1242–1252

    Article  CAS  Google Scholar 

  10. Siren H, Vuorensola K (2001) J Microcolumn Sep 13:126–133

    Article  CAS  Google Scholar 

  11. Vuorensola K, Siren H (2000) J Chromatogr A 895:317–327

    Article  CAS  Google Scholar 

  12. Weng Q, Xu G, Yuan K, Tang P (2006) J Chromatogr B 835:55–61

    Article  CAS  Google Scholar 

  13. Hsieh MM, Chang HT (2006) J Chromatogr A 1102:302–308

    Article  CAS  Google Scholar 

  14. Vuorensola K, Siren H, Kostiainen R, Kotiaho T (2002) J Chromatogr A 979:179–189

    Article  CAS  Google Scholar 

  15. Kartsova LA, Bessonova EA, Sidorova AA, Kazakov VA, Tveryanovich IA, Velikanova LI (2004) Russ J Appl Chem 77(7):1150–1155

    Article  CAS  Google Scholar 

  16. Park YH, Zhang X, Rubakhin SS, Sweedler JV (1999) Anal Chem 71:4997–5002

    Article  CAS  Google Scholar 

  17. Paquette DM, Sing R, Banks PR, Waldron KC (1998) J Chromatogr B 714:47–57

    Article  CAS  Google Scholar 

  18. Chen DC, Zhan DZ, Cheng CW, Liu AC, Chen CH (2001) J Chromatogr B 750:33–39

    Article  CAS  Google Scholar 

  19. Peaston RT, Weinkove C (2004) Ann Clin Biochem 41:17–38

    Article  CAS  Google Scholar 

  20. Thomas DH, Taylor JD, Barnaby OS, Hage DS (2008) Clin Chim Acta 398:63–69

    Article  CAS  Google Scholar 

  21. Kenney A, Fowell S (1992) Methods in molecular biology, vol 11. Humana, Totowa (Chapter 4)

    Google Scholar 

  22. Easley CJ, Jin L, Elgstoen KBP, Jellum E, Landers JP, Ferrance JP (2003) J Chromatogr A 1004:29–37

    Article  CAS  Google Scholar 

  23. Xiao-Chuan L (2006) Chin J Chromatogr 24(1):73–80

    Article  Google Scholar 

  24. Cannizzo C, Amigoni-Gerbier S, Larpent C (2005) Polymer 46:1269–1276

    Article  CAS  Google Scholar 

  25. Li F, Zhao X, Wang W, Xu G (2006) Anal Chim Acta 580:181–187

    Article  CAS  Google Scholar 

  26. Kitahara K-I, Noguchi Y, Itoh S, Chiba N, Tohyama T, Nagashima K, Hanada T, Yoshihama I, Arai S (2009) J Chromatogr A 1216:7415–7421

    Article  CAS  Google Scholar 

  27. Ivanov AE, Larsson H, Galaev IY, Mattiasson B (2004) Polymer 45:2495–2505

    Article  CAS  Google Scholar 

  28. Ren L, Liu Z, Dong M, Ye M, Zou H (2009) J Chromatogr A 1216:4768–4774

    Article  CAS  Google Scholar 

  29. Kahraman G, Beskardes O, Rzaev ZMO, Piskin E (2004) Polymer 45:5813–5828

    Article  CAS  Google Scholar 

  30. Letsinger RL, Hamilton SB (1959) J Amer Chem Soc 81:3009–3012

    Article  CAS  Google Scholar 

  31. Buchmeiser MR (2007) Polymer 48:2187–2198

    Article  CAS  Google Scholar 

  32. Augustin V, Jardy A, Gareil P, Hennion M-C (2006) J Chromatogr A 1119:80–87

    Article  CAS  Google Scholar 

  33. Urban J, Jandera P, Schoenmakers P (2007) J Chromatogr A 1150:279–289

    Article  CAS  Google Scholar 

  34. Yang Y, Li C, Lee KH, Craighead HG (2005) Electrophoresis 26:3622–3630

    Article  CAS  Google Scholar 

  35. Svec F, Tennikova TB, Deyl Z (2003) Monolithic materials: miniature and microchip technologies. Elsevier, Amsterdam

    Google Scholar 

  36. Xie S, Svec F, Frechet JMJ (1998) Chem Mater 10:4072–4078

    Article  CAS  Google Scholar 

  37. Svec F (2006) J Chromatogr B 841:52–64

    Article  CAS  Google Scholar 

  38. Wu Q, Bienvenue JM, Hassan BJ, Kwok YC, Giordano BC, Norris PM, Landers JP, Ferrance JP (2006) Anal Chem 78:5704–5710

    Article  CAS  Google Scholar 

  39. Wen J, Guillo C, Ferrance JP, Landers JP (2006) Anal Chem 78:1673–1681

    Article  CAS  Google Scholar 

  40. Schaller D, Hilder EF, Haddad PR (2006) Anal Chim Acta 556:104–111

    Article  CAS  Google Scholar 

  41. Marchiarullo DJ, Lim JY, Vaksman Z, Ferrance JP, Putcha L, Landers JP (2008) J Chromatogr A 1200:198–203

    Article  CAS  Google Scholar 

  42. Kutter JP, Jacobson SC, Ramsey JM (2000) J Microcolumn Sep 12:93–97

    Article  CAS  Google Scholar 

  43. Tuomikoski S, Virkkala N, Rovio S, Hokkanen A, Siren H, Franssila S (2006) J Chromatogr A 1111:258–266

    Article  CAS  Google Scholar 

  44. Legendre LA, Bienvenue JM, Roper MG, Ferrance JP, Landers JP (2006) Anal Chem 78:1444–1451

    Article  CAS  Google Scholar 

  45. Bienvenue JM, Legendre LA, Ferrance JP, Landers JP (2010) Forensic Sci Int Gen 4:178–186

    Article  CAS  Google Scholar 

  46. SCHOTT North America, Inc. (2010) http://www.us.schott.com/borofloat/english/attribute/optical/index.html

  47. Chang H-T, Yeung ES (1995) Anal Chem 67:1079–1083

    Article  CAS  Google Scholar 

  48. Zhang X, Sweedler JV (2001) Anal Chem 73:5620–5624

    Article  CAS  Google Scholar 

  49. Ramsey JD, Collins GE (2005) Anal Chem 77:6664–6670

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors would like to thank TUBITAK (The Scientific and Technological Research Council of Turkey) for providing grant to this project, and Chemistry Departments of the University of Virginia (USA) and Hacettepe University (Turkey).

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Authors and Affiliations

  1. The Institute for Graduate Studies in Science and Engineering, Bioengineering Subdivision, Hacettepe University, 06532 Beytepe, Ankara, Turkey

    Cafer Cakal

  2. Department of Chemistry, University of Virginia, Charlottesville, VA, 22904-4113, USA

    Jerome P. Ferrance & James P. Landers

  3. Faculty of Science, Department of Chemistry, Hacettepe University, 06532 Beytepe, Ankara, Turkey

    Perihan Caglar

Authors
  1. Cafer Cakal
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  2. Jerome P. Ferrance
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  3. James P. Landers
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  4. Perihan Caglar
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Correspondence to Perihan Caglar.

Additional information

Published in the special issue Optical Biochemical and Chemical Sensors (Europtrode X) with Guest Editor Jiri Homola.

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Cakal, C., Ferrance, J.P., Landers, J.P. et al. Development of a micro-total analysis system (μ-TAS) for the determination of catecholamines. Anal Bioanal Chem 398, 1909–1917 (2010). https://doi.org/10.1007/s00216-010-3998-8

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  • DOI: https://doi.org/10.1007/s00216-010-3998-8

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