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SERS-based plasmonic nanobiosensing in single living cells

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Abstract

In this paper, we describe the development and application of a pH-sensitive plasmonics-active fiber-optic nanoprobe suitable for intracellular bioanalysis in single living human cells using surface-enhanced Raman scattering (SERS) detection. The effectiveness and usefulness of SERS-based fiber-optic nanoprobes are illustrated by measurements of intracellular pH in HMEC-15/hTERT immortalized “normal” human mammary epithelial cells and PC-3 human prostate cancer cells. The results indicate that fiber-optic nanoprobe insertion and interrogation provide a sensitive and selective means to monitor cellular microenvironments at the single cell level.

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Acknowledgments

This research was financially supported by the National Institute of Health (NIH R01 EB006201 and NIH R01 ES014774) and the Department of Justice.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Duke University, 136 Hudson Hall, P. O. Box 90281, Durham, NC, 27708, USA

    Jonathan P. Scaffidi, Molly K. Gregas & Tuan Vo-Dinh

  2. Fitzpatrick Institute for Photonics, Duke University, 305 Teer Building, P. O. Box 90271, Durham, NC, 27708, USA

    Jonathan P. Scaffidi, Molly K. Gregas, Victoria Seewaldt & Tuan Vo-Dinh

  3. Division of Medical Oncology, Duke School of Medicine, DUMC 2628 Room 221A MSRB, Durham, NC, 27710, USA

    Victoria Seewaldt

  4. Department of Chemistry, Duke University, 124 Science Drive, P. O. Box 90354, Durham, NC, 27708, USA

    Tuan Vo-Dinh

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  1. Jonathan P. Scaffidi
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  2. Molly K. Gregas
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  4. Tuan Vo-Dinh
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Correspondence to Tuan Vo-Dinh.

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Scaffidi, J.P., Gregas, M.K., Seewaldt, V. et al. SERS-based plasmonic nanobiosensing in single living cells. Anal Bioanal Chem 393, 1135–1141 (2009). https://doi.org/10.1007/s00216-008-2521-y

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  • DOI: https://doi.org/10.1007/s00216-008-2521-y

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