Abstract
This study reports an effort to synthesize biocompatible zinc oxide nanoparticles using sol-gel method and its influence on hematological and serological profile of Catla catla fish. Hexagonal wurtzite structure and crystallite size of ZnO-NPs was identified by using XRD in the range of 19 to 20 nm. Moreover, the irregular and non-uniform surface of these NPs was found using SEM. The different stretched and vibrational mode (ZnO, OH, CO, and H–O–H) was identified by using FTIR analysis. UV-visible spectroscopy confirmed absorbance of the blue shift in the range 340 nm. Bioassay of ZnO-NPs on Catla catla was performed and nano ZnO was given through intraperitoneal injections at 0, 25, 50, 75, and 100 μg/g body weight doses. Analysis of fish blood samples indicated an increase in WBCs and leukocytes while the differential effect on monocytes. On the other hand in response to varying ZnO concentrations, an increase in RBCs, hemoglobin, and HCT was evident. Serum analysis revealed an increase in urea concentration while a reduction in creatinine, ALT, and AST. In an overall assessment, nano-ZnO supplementation at 25 to 100 μg/g body weight differentially affected hematological and serum biochemical profile of thaila fish.
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Abbreviations
- ALT:
-
Alanine aminotransferase
- AST:
-
Aspartate aminotransferase
- ENPs:
-
Engineered nanoparticles
- FeO:
-
Iron oxide
- FTIR:
-
Fourier-transform infrared spectroscopy
- HCT:
-
Hematocrit
- IP:
-
Intraperitoneal
- MCH:
-
Mean corpuscular hemoglobin
- MCHC:
-
Mean corpuscular hemoglobin concentration
- MCV:
-
Mean corpuscular volume
- MPV:
-
Mean platelet volume
- NADPH:
-
Nicotinamide adenine dinucleotide phosphate
- PCT:
-
Platelet crit
- PDW:
-
Platelet distribution width
- P-LCR:
-
Platelet large cell ratio
- RBCs:
-
Red blood cells
- RDW:
-
Red blood cell distribution width
- SEM:
-
Scanning electron microscope
- WBCs:
-
White blood cells
- ZnO:
-
Zinc oxide
- ZnO-NPs:
-
Zinc oxide nanoparticles
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Acknowledgments
The authors would like to extend their sincere appreciation to HEC under Project Numbers 8466/Punjab/NRPU/R&D/HEC/2017 (Dr Tariq Munir) Department of Physics Government College University Faisalabad Pakistan.
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Arslan Mahmood and Fahad Shafiq conceived and designed research. Tariq Munir and Arslan Mahmood synthesized and characterized the ZnO nanoparticles and wrote the initial manuscript draft. Muhammad Latif conducted bioassay, analyzed hematological and serological profile, performed statistical analyses of data, and wrote results of fish bioassay. Arif Malik and Fahad Shafiq critically revised and updated the manuscript. All the authors read the manuscript and approved its final submission. All data were generated in-house and that no paper mill was used.
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Munir, T., Latif, M., Mahmood, A. et al. Influence of IP-injected ZnO-nanoparticles in Catla catla fish: hematological and serological profile. Naunyn-Schmiedeberg's Arch Pharmacol 393, 2453–2461 (2020). https://doi.org/10.1007/s00210-020-01955-6
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DOI: https://doi.org/10.1007/s00210-020-01955-6