Skip to main content

Advertisement

Log in

Influence of IP-injected ZnO-nanoparticles in Catla catla fish: hematological and serological profile

  • Original Article
  • Published:
Naunyn-Schmiedeberg's Archives of Pharmacology Aims and scope Submit manuscript

Abstract

This study reports an effort to synthesize biocompatible zinc oxide nanoparticles using sol-gel method and its influence on hematological and serological profile of Catla catla fish. Hexagonal wurtzite structure and crystallite size of ZnO-NPs was identified by using XRD in the range of 19 to 20 nm. Moreover, the irregular and non-uniform surface of these NPs was found using SEM. The different stretched and vibrational mode (ZnO, OH, CO, and H–O–H) was identified by using FTIR analysis. UV-visible spectroscopy confirmed absorbance of the blue shift in the range 340 nm. Bioassay of ZnO-NPs on Catla catla was performed and nano ZnO was given through intraperitoneal injections at 0, 25, 50, 75, and 100 μg/g body weight doses. Analysis of fish blood samples indicated an increase in WBCs and leukocytes while the differential effect on monocytes. On the other hand in response to varying ZnO concentrations, an increase in RBCs, hemoglobin, and HCT was evident. Serum analysis revealed an increase in urea concentration while a reduction in creatinine, ALT, and AST. In an overall assessment, nano-ZnO supplementation at 25 to 100 μg/g body weight differentially affected hematological and serum biochemical profile of thaila fish.

Graphical abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Abbreviations

ALT:

Alanine aminotransferase

AST:

Aspartate aminotransferase

ENPs:

Engineered nanoparticles

FeO:

Iron oxide

FTIR:

Fourier-transform infrared spectroscopy

HCT:

Hematocrit

IP:

Intraperitoneal

MCH:

Mean corpuscular hemoglobin

MCHC:

Mean corpuscular hemoglobin concentration

MCV:

Mean corpuscular volume

MPV:

Mean platelet volume

NADPH:

Nicotinamide adenine dinucleotide phosphate

PCT:

Platelet crit

PDW:

Platelet distribution width

P-LCR:

Platelet large cell ratio

RBCs:

Red blood cells

RDW:

Red blood cell distribution width

SEM:

Scanning electron microscope

WBCs:

White blood cells

ZnO:

Zinc oxide

ZnO-NPs:

Zinc oxide nanoparticles

References

  • Adhikari S, Sarkar B, Chatterjee A, Mahapatra CT, Ayyappan S (2004) Effects of cypermethrin and carbofuran on certain haematological parameters and prediction of their recovery in a freshwater teleost, Labeo rohita (Hamilton). Ecotoxicol Environ Saf 58(2):220–226

    Article  CAS  Google Scholar 

  • Adhikari BN, Wall DH, Adams BJ (2010) Effect of slow desiccation and freezing on gene transcription and stress survival of an Antarctic nematode. J Exp Biol 213(11):1803–1812

    Article  CAS  Google Scholar 

  • Ahmed R, Tariq M, Ali I, Asghar R, Khanam PN, Augustine R, Hasan A (2018) Novel electrospun chitosan/polyvinyl alcohol/zinc oxide nanofibrous mats with antibacterial and antioxidant properties for diabetic wound healing. Int J Biol Macromol 120:385–393

    Article  CAS  Google Scholar 

  • Akram MW, Alam MF, Ji HN, Mahmood A, Munir T, Iqbal MZ, Wu AG (2019) Chitosan blend iron oxide nanostructure-based biosensor for healthy & malignant tissue glucose/urea detection. In: IOP Conference Series: Materials Science and Engineering (Vol. 474, No. 1, p. 012060). IOP Publishing

  • Banaee M, Tahery S, Nematdoost Haghi B, Shahafve S, Vaziriyan M (2019) Blood biochemical changes in common carp (Cyprinus carpio) upon co-exposure to titanium dioxide nanoparticles and paraquat. Iran J Fish Sci 18(2):242–255

    Google Scholar 

  • Batley GE, Kirby JK, McLaughlin MJ (2013) Fate and risks of nanomaterials in aquatic and terrestrial environments. Acc Chem Res 46(3):854–862

    Article  CAS  Google Scholar 

  • Brown C, Day RL (2002) The future of stock enhancements: lessons for hatchery practice from conservation biology. Fish Fish 3(2):79–94

    Article  Google Scholar 

  • Bundschuh M, Filser J, Lüderwald S, McKee MS, Metreveli G, Schaumann GE, Wagner S (2018) Nanoparticles in the environment: where do we come from, where do we go to? Environ Sci Eur 30(1):1–17

    Article  CAS  Google Scholar 

  • Carvalho CS, Fernandes MN (2006) Effect of temperature on copper toxicity and haematological responses in the neotropical fish Prochilodus scrofa at low and high pH. Aquaculture 251(1):109–117

    Article  CAS  Google Scholar 

  • Chupani L, Niksirat H, Velíšek J, Stará A, Hradilová Š, Kolařík J, Panáček A, Zusková E (2018) Chronic dietary toxicity of zinc oxide nanoparticles in common carp (Cyprinus carpio L.): tissue accumulation and physiological responses. Ecotoxicol Environ Saf 147:110–116

    Article  CAS  Google Scholar 

  • Datta S, Saha DR, Ghosh D, Majumdar T, Bhattacharya S, Mazumder S (2007) Sub-lethal concentration of arsenic interferes with the proliferation of hepatocytes and induces in vivo apoptosis in Clarias batrachus L. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 145(3):339–349

    Google Scholar 

  • Devineni SB, Suneetha O, Harshavardhan N (2015) Study of platelet count in malaria patients and the correlation between the presence and severity of platelet count with type of malaria. J Evol Med Dent Sci 4(67):11734–11746

    Article  Google Scholar 

  • Gandhi PR, Jayaseelan C, Mary RR, Mathivanan D, Suseem SR (2017) Acaricidal, pediculicidal and larvicidal activity of synthesized ZnO nanoparticles using Momordica charantia leaf extract against blood feeding parasites. Exp Parasitol 181:47–56

    Article  CAS  Google Scholar 

  • Gherab K, Al-Douri Y, Voon CH, Hashim U, Ameri M, Bouhemadou A (2017) Aluminium nanoparticles size effect on the optical and structural properties of ZnO nanostructures synthesized by spin-coating technique. Results Phys 7:1190–1197

    Article  Google Scholar 

  • Giordo R, Nasrallah GK, Al-Jamal O, Paliogiannis P, Pintus G (2020) Resveratrol inhibits oxidative stress and prevents mitochondrial damage induced by zinc oxide nanoparticles in Zebrafish (Danio rerio). Int J Mol Sci 21(11):3838

    Article  CAS  Google Scholar 

  • Hernández-Moreno D, Valdehita A, Conde E, Rucandio I, Navas JM, Fernández-Cruz ML (2019) Acute toxic effects caused by the co-exposure of nanoparticles of ZnO and Cu in rainbow trout. Sci Total Environ 687:24–33

    Article  CAS  Google Scholar 

  • Hou J, You G, Xu Y, Wang C, Wang P, Miao L, Yang Y (2016) Antioxidant enzyme activities as biomarkers of fluvial biofilm to ZnO NPs ecotoxicity and the integrated biomarker responses (IBR) assessment. Ecotoxicol Environ Saf 133:10–17

    Article  CAS  Google Scholar 

  • Huo Z, Wu CH, Zhu Z, Zhao Y (2015) Advanced materials and nanotechnology for sustainable energy development. J Nanotechnol:2015

  • Husen A (2019) Natural product-based fabrication of zinc-oxide nanoparticles and their applications. In: Nanomaterials and plant potential. Springer, Cham, pp 193–219

    Chapter  Google Scholar 

  • Jacobson-Kram D, Keller KA (2001) Toxicology testing handbook: principles. In: Applications and data interpretation, 2nd edn. Marcel Decker, New York

    Google Scholar 

  • Jangir LK, Kumari Y, Kumar A, Kumar M, Awasthi K (2017) Investigation of luminescence and structural properties of ZnO nanoparticles, synthesized with different precursors. Mater Chem Front 1(7):1413–1421

    Article  Google Scholar 

  • Kavitha C, Malarvizhi A, Kumaran SS, Ramesh M (2010) Toxicological effects of arsenate exposure on hematological, biochemical and liver transaminases activity in an Indian major carp, Catla catla. Food Chem Toxicol 48(10):2848–2854

    Article  CAS  Google Scholar 

  • Mahananda HB (2014) Alterations in some haemato-bio-chemical parameters of a fresh water, air breathing fish, Channa punctatus (Bloch) under the stress of chronic, sub-lethal dose of nickel. Biolife 2(4):1392–1397

    Google Scholar 

  • Mishra SK, Srivastava RK, Prakash SG (2012) ZnO nanoparticles: structural, optical and photoconductivity characteristics. J Alloys Compd 539:1–6

    Article  CAS  Google Scholar 

  • Munir T, Rizwan M, Kashif M, Shahzad A, Ali S, Amin N, Imran M (2018) Effect of zinc oxide nanoparticles on the growth and Zn uptake in wheat (Triticum aestivum L.) by seed priming method. Digest Journal of Nanomaterials & Biostructures (DJNB) 13(1)

  • Munir T, Mahmood A, Fakhar-e-Alam M, Imran M, Sohail A, Amin N, Mahmood K (2019) Treatment of breast cancer with capped magnetic-NPs induced hyperthermia therapy. J Mol Struct 1196:88–95

    Article  CAS  Google Scholar 

  • Parra MR, Haque FZ (2014) Structural and optical properties of poly-vinylpyrrolidone modified ZnO nanorods synthesized through simple hydrothermal process. Optik 125(17):4629–4632

    Article  CAS  Google Scholar 

  • Peng C, Zhang W, Gao H, Li Y, Tong X, Li K, Zhu X, Wang Y, Chen Y (2017) Behavior and potential impacts of metal-based engineered nanoparticles in aquatic environments. Nanomaterials 7(1):21

    Article  CAS  Google Scholar 

  • Riaz-ul-Haq M, Javeed R, Iram S, Rasheed MA, Amjad M, Iqbal F (2018) Effect of Diafenthiuron exposure under short and long term experimental conditions on hematology, serum biochemical profile and elemental composition of a non-target organism, Labeo rohita. Environ Toxicol Pharmacol 62:40–45

    Article  CAS  Google Scholar 

  • Santhoshkumar J, Kumar SV, Rajeshkumar S (2017) Synthesis of zinc oxide nanoparticles using plant leaf extract against urinary tract infection pathogen. Resource-Efficient Technol 3(4):459–465

    Article  Google Scholar 

  • Schilling K, Bradford B, Castelli D, Dufour E, Nash JF, Pape W, Schellauf F (2010) Human safety review of “nano” titanium dioxide and zinc oxide. Photochem Photobiol Sci 9(4):495–509

    Article  CAS  Google Scholar 

  • Sette LHBC, de Almeida Lopes EP (2014) Liver enzymes serum levels in patients with chronic kidney disease on hemodialysis: a comprehensive review. Clinics (Sao Paulo) 69(4):271–278

    Article  Google Scholar 

  • Shafiq F, Iqbal M, Ali M, Ashraf MA (2019) Seed pre-treatment with polyhydroxy fullerene nanoparticles confer salt tolerance in wheat through upregulation of H2O2 neutralizing enzymes and phosphorus uptake. J Soil Sci Plant Nutr 19(4):734–742

    Article  CAS  Google Scholar 

  • Solomon SG, Okomoda VT (2012) Effects of photoperiod on the haematological parameters of Clarias gariepinus fingerlings reared in water circulatory system. J Stress Physiol Biochem 8(3)

  • Talam S, Karumuri SR, Gunnam N (2012) Synthesis, characterization, and spectroscopic properties of ZnO nanoparticles. ISRN Nanotechnology 2012:1–6

    Article  CAS  Google Scholar 

  • Thangeeswari T, George AT, Kumar AA (2016) Optical properties and FTIR studies of cobalt doped ZnO nanoparticles by simple solution method. Indian J Sci Technol 9(1)

  • Vignesh S, Suganthi S, Sundar JK, Raj V, Devi PRI (2019) Highly efficient visible light photocatalytic and antibacterial performance of PVP capped Cd: Ag: ZnO photocatalyst nanocomposites. Appl Surf Sci 479:914–929

    Article  CAS  Google Scholar 

  • Wang C, Lv J, Ren Y, Zhou Q, Chen J, Zhi T, Jin L (2016) Cotton fabric with plasma pretreatment and ZnO/carboxymethyl chitosan composite finishing for durable UV resistance and antibacterial property. Carbohydr Polym 138:106–113

    Article  CAS  Google Scholar 

  • Xiong D, Fang T, Yu L, Sima X, Zhu W (2011) Effects of nano-scale TiO2, ZnO and their bulk counterparts on zebra fish: acute toxicity, oxidative stress and oxidative damage. Sci Total Environ 409(8):1444–1452

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to extend their sincere appreciation to HEC under Project Numbers 8466/Punjab/NRPU/R&D/HEC/2017 (Dr Tariq Munir) Department of Physics Government College University Faisalabad Pakistan.

Author information

Authors and Affiliations

Authors

Contributions

Arslan Mahmood and Fahad Shafiq conceived and designed research. Tariq Munir and Arslan Mahmood synthesized and characterized the ZnO nanoparticles and wrote the initial manuscript draft. Muhammad Latif conducted bioassay, analyzed hematological and serological profile, performed statistical analyses of data, and wrote results of fish bioassay. Arif Malik and Fahad Shafiq critically revised and updated the manuscript. All the authors read the manuscript and approved its final submission. All data were generated in-house and that no paper mill was used.

Corresponding authors

Correspondence to Tariq Munir or Muhammad Latif.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

ESM 1

(DOCX 13 kb)

ESM 2

(XLSX 64 kb)

ESM 3

(XLSX 14 kb)

ESM 4

(XLSX 44 kb)

ESM 5

(XLSX 34 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Munir, T., Latif, M., Mahmood, A. et al. Influence of IP-injected ZnO-nanoparticles in Catla catla fish: hematological and serological profile. Naunyn-Schmiedeberg's Arch Pharmacol 393, 2453–2461 (2020). https://doi.org/10.1007/s00210-020-01955-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00210-020-01955-6

Keywords

Navigation