Abstract
The mitochondrial atpA gene sequence of the normal fertile sugarbeet (cv ‘TK81-0’) exists in one full-length version and one truncated version, both of which are present in normal stoichiometry and have a 406-bp segment in common. The PCR approach as well as prolonged exposure of Southern blots indicates that the products of the recombination across the 406-bp repeat are present in substoichiometric amounts in the ‘TK81-0’ genome. Intriguingly, one of these substoichiometric sequence arrangements was revealed to be preferentially amplified in an evolutionary lineage that led to a cytoplasmic male-sterile variant [I-12CMS(2)] in wild beets. We also found the 406-bp repeat to be part of a 6.5-kb repeat in the mitochondrial genome of I-12CMS(2). This 6.5-kb duplication is likely to involve recombination between two sets of repeats (the above-mentioned 406-bp repeat and a 7-bp repeat) in an ancestral beet mitochondria.
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Received: 4 October 1997 / Accepted: 31 October 1997
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Senda, M., Onodera, Y. & Mikami, T. Recombination events across the atpA-associated repeated sequences in the mitochondrial genomes of beets. Theor Appl Genet 96, 964–968 (1998). https://doi.org/10.1007/s001220050827
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DOI: https://doi.org/10.1007/s001220050827