Abstract
Key Message
Many deletions of the wheat Della ( Rht - B1 ) gene and its flanking regions were isolated in a simple phenotypic screen, and characterised by modified analysis of SNP hybridisation data and cytogenetics.
Abstract
In a dwarf wheat suppressor screen, many tall ‘revertants’ were isolated following mutagenesis of a severely dwarfed (Rht-B1c) hexaploid wheat. About 150 lines were identified as putative deletions of Rht-B1c, based on the PCR analysis. Southern blot hybridisation established that most of them lacked the Rht-B1 gene, but retained the homoeologues Rht-A1 and Rht-D1. PCR assays were developed for orthologues of two genes that flank Rht-1/Della in the genomes of the model species Brachypodium and rice. Deletion of the B-genome-specific homoeologues of these two genes was confirmed in the Rht-B1 deletion lines, indicating loss of more than a single gene. SNP chip hybridisation analysis established the extents of deletion in these lines. Based on the synteny with Brachypodium chromosomes 1 and 4 g, and rice chromosomes 3g and 11g, notional deletion maps were established. The deletions ranged from interstitial deletions of 4BS through to loss of all 4BS markers. There were also instances, where all 4BS and 4BL markers were lost, and these lines had poor fertility and narrow stems and leaves. Cytogenetic studies on selected lines confirmed the loss of portions of 4BS in lines that lacked most or all 4BS markers. They also confirmed that lines lacking both 4BS and 4BL markers were nullisomics for 4B. These nested deletion lines share a common genetic background and will have applications in assigning markers to regions of 4BS as well as to 4BL. The potential for this type of analysis in other regions of the wheat genome is discussed.
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References
Chandler PM, Harding CA (2013) ‘Overgrowth’ mutants in barley and wheat, new alleles and phenotypes of the ‘Green Revolution’ Della gene. J Exp Bot 64:1603–1613
Chandler PM, Robertson M (1999) Gibberellin dose-response curves and the characterization of dwarf mutants of barley. Plant Physiol 120:623–632
Chandler PM, Marion-Poll A, Ellis M, Gubler F (2002) Mutants at the Slender1 locus of barley cv Himalaya. Molecular and physiological characterization. Plant Physiol 129:181–190
Endo TR (1990) Gametocidal chromosomes and their induction of chromosome mutations in wheat. Jpn J Genet 65:135–152
Endo TR, Tsunewaki K (1975) Sterility of common wheat with Aegilops-Triuncialis cytoplasm. J Hered 66:13–18
Endo TR, Gill BS (1996) The deletion stocks of common wheat. J Hered 87:295–307
Endo TR, Mukai Y, Yamamoto M, Gill BS (1991) Physical mapping of a male-fertility gene of common wheat. Jpn J Genet 66:291–295
Harberd NP, King KE, Carol P, Cowling RJ, Peng JR, Richards DE (1998) Gibberellin, inhibitor of an inhibitor of …? BioEssays 20:1001–1008
Ikeda A, Ueguchi-Tanaka M, Sonoda Y, Kitano H, Koshioka M, Futsuhara Y, Matsuoka M, Yamaguchi J (2001) Slender rice, a constitutive gibberellin response mutant, is caused by a null mutation of the SLR1 gene, an ortholog of the height-regulating gene GAI/RGA/RHT/D8. Plant Cell 13:999–1010
Joshi GP, Li JJ, Nasuda S, Endo TR (2013) Development of a self-fertile ditelosomic line for the long arm of chromosome 4B and its characterization using SSR markers. Genes Genet Syst 88:311–314
Lagudah ES, Appels R, Brown AHD, McNeil D (1991) The molecular-genetic analysis of Triticum-tauschii, the D-genome donor to hexaploid wheat. Genome 34:375–386
Pedersen C, Langridge P (1997) Identification of the entire chromosome complement of bread wheat by two-color FISH. Genome 40:589–593
Pedersen C, Rasmussen SK, LindeLaursen I (1996) Genome and chromosome identification in cultivated barley and related species of the Triticeae (Poaceae) by in situ hybridization with the GAA-satellite sequence. Genome 39:93–104
Peng JR, Carol P, Richards DE, King KE, Cowling RJ, Murphy GP, Harberd NP (1997) The Arabidopsis GAI gene defines a signaling pathway that negatively regulates gibberellin responses. Gene Dev 11:3194–3205
Peng JR, Richards DE, Hartley NM, Murphy GP, Devos KM, Flintham JE, Beales J, Fish LJ, Worland AJ, Pelica F, Sudhakar D, Christou P, Snape JW, Gale MD, Harberd NP (1999) ‘Green revolution’ genes encode mutant gibberellin response modulators. Nature 400:256–261
Qi L, Echalier B, Friebe B, Gill BS (2003) Molecular characterization of a set of wheat deletion stocks for use in chromosome bin mapping of ESTs. Funct Integr Genomic 3:39–55
Rayburn AL, Gill BS (1986) Isolation of a D-genome specific repeated DNA sequence from Aegilops squarrosa. Plant Mol Biol 4:102–109
Riley R, Kimber G, Chapman V (1961) Evolution of diploid-like meiosis of polyploid wheat. Ann Hum Genet 25:173
Sears ER (1954) The aneuploids of common wheat. Research Bulletin. Missouri Agricultural Experiment Station. Issue 572, p 58
Sears E (1966) Chromosome mapping with the aid of telocentrics. Hereditas 2:370–381
Storlie EW, Talbert LE (1993) Cause of tall off-types in a semidwarf spring wheat. Crop Sci 33:1131–1135
Storlie EW, Xie H, Talbert LE (1996) Tall off-types in semidwarf spring wheat with height-reducing genes Rht1 and Rht2. Crop Sci 36:1521–1522
Wang S, Wong D, Forrest K, Allen A, Huang BE, Maccaferri M, Salvi S, Milner SG, Cattivelli L, Mastrangelo AM, Whan A, Stephen S, Barker G, Wieseke R, Plieske J, International Wheat Genome Sequencing Consortium, Lillemo M, Mather D, Appels R, Dolferus R, Brown-Guedira G, Korol A, Akhunova AR, Feuillet C, Salse J, Morgante M, Pozniak C, Luo MC, Dvorak J, Morell M, Dubcovsky J, Ganal M, Tuberosa R, Lawley C, Mikoulitch I, Cavanagh C, Edwards KJ, Hayden M, Akhunov E (2014) Characterization of polyploid wheat genomic diversity using a high-density 90,000 single nucleotide polymorphism array. Plant Biotechnol J 12:787–796
Worland AJ, Law CN (1985) Aneuploidy in semi dwarf wheat-varieties. Euphytica 34:317–327
Wu J, Kong X, Wan J, Liu X, Zhang X, Guo X, Zhou R, Zhao G, Jing R, Fu X, Jia J (2011) Dominant and pleiotropic effects of a GAI gene in wheat results from a lack of interaction between DELLA and GID1. Plant Physiol 157:2120–2130
Zhang P, Friebe B, Lukaszewski AJ, Gill BS (2001) The centromere structure in Robertsonian wheat-rye translocation chromosomes indicates that centric breakage-fusion can occur at different positions within the primary constriction. Chromosoma 110:335–344
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AM was supported by a Ph.D. scholarship from the Australian Grains Research and Development Corporation.
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Communicated by X. Xia.
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122_2016_2763_MOESM1_ESM.xlsx
Supplementary Fig. 1 Details of individual wheat markers and their presence (green) or absence (blue) in deletion lines. NC = no genotype call. This data are the basis for Fig. 4. (XLSX 73 kb)
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Miraghazadeh, A., Zhang, P., Harding, C. et al. The use of SNP hybridisation arrays and cytogenetics to characterise deletions of chromosome 4B in hexaploid wheat (Triticum aestivum L.). Theor Appl Genet 129, 2151–2160 (2016). https://doi.org/10.1007/s00122-016-2763-6
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DOI: https://doi.org/10.1007/s00122-016-2763-6