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Development of genic cleavage markers in association with seed glucosinolate content in canola

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The orthologues of Arabidopsis involved in seed glucosinolates metabolism within QTL confidence intervals were identified, and functional markers were developed to facilitate breeding for ultra-low glucosinolates in canola.

Abstract

Further reducing the content of seed glucosinolates will have a positive impact on the seed quality of canola (Brassica napus). In this study 43 quantitative trait loci (QTL) for seed glucosinolate (GSL) content in a low-GSL genetic background were mapped over seven environments in Germany and China in a doubled haploid population from a cross between two low-GSL oilseed rape parents with transgressive segregation. By anchoring these QTL to the reference genomes of B. rapa and B. oleracea, we identified 23 orthologues of Arabidopsis involved in GSL metabolism within the QTL confidence intervals. Sequence polymorphisms between the corresponding coding regions of the parental lines were used to develop cleaved amplified polymorphic site markers for two QTL-linked genes, ISOPROPYLMALATE DEHYDROGENASE1 and ADENOSINE 5′-PHOSPHOSULFATE REDUCTASE 3. The genic cleavage markers were mapped in the DH population into the corresponding intervals of QTL explaining 3.36–6.88 and 4.55–8.67 % of the phenotypic variation for seed GSL, respectively. The markers will facilitate breeding for ultra-low seed GSL content in canola.

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Acknowledgments

We thank Norddeutsche Pflanzenzucht Hans-Georg Lembke KG, Germany, for developing the DH population. This study was supported by grant 973 (2015CB150201), the Key Projects in the National Science and Technology (2014BAD01B07), the Fundamental Research Funds for the Central Universities (XDJK2013A013, XDJK2014C148, XDJK2014B036, XDJK2014A015, SWU113106, SWU113065) and NSFC (31171585, 31471529).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. College of Agronomy and Biotechnology, Southwest University, 400716, Chongqing, China

    Ying Fu, Kun Lu, Lunwen Qian, Jiaqin Mei, Dayong Wei, Xuhui Peng, Xinfu Xu, Jiana Li & Wei Qian

  2. Department of Plant Breeding, IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany

    Ying Fu, Lunwen Qian, Jiaqin Mei & Rod J. Snowdon

  3. Norddeutsche Pflanzenzucht Hans-Georg Lembke KG, 24363, Hohenlieth, Germany

    Martin Frauen & Felix Dreyer

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  1. Ying Fu
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  2. Kun Lu
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Correspondence to Rod J. Snowdon or Wei Qian.

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Communicated by M. L. Federico.

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Fu, Y., Lu, K., Qian, L. et al. Development of genic cleavage markers in association with seed glucosinolate content in canola. Theor Appl Genet 128, 1029–1037 (2015). https://doi.org/10.1007/s00122-015-2487-z

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