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Identification of a candidate gene for the wheat endopeptidase Ep-D1 locus and two other STS markers linked to the eyespot resistance gene Pch1

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Abstract

Wheat is prone to strawbreaker foot rot (eyespot), a fungal disease caused by Oculimacula yallundae and O. acuformis. The most effective source of genetic resistance is Pch1, a gene derived from Aegilops ventricosa. The endopeptidase isozyme marker allele Ep-D1b, linked to Pch1, has been shown to be more effective for tracking resistance than DNA-based markers developed to date. Therefore, we sought to identify a candidate gene for Ep-D1 as a basis for a DNA-based marker. Comparative mapping suggested that the endopeptidase loci Ep-D1 (wheat), enp1 (maize), and Enp (rice) were orthologous. Since the product of the maize endopeptidase locus enp1 has been shown to exhibit biochemical properties similar to oligopeptidase B purified from E. coli, we reasoned that Ep-D1 may also encode an oligopeptidase B. Consistent with this hypothesis, a sequence-tagged-site (STS) marker, Xorw1, derived from an oligopeptidase B-encoding wheat expressed-sequence-tag (EST) showed complete linkage with Ep-D1 and Pch1 in a population of 254 recombinant inbred lines (RILs) derived from a cross between wheat cultivars Coda and Brundage. Two other STS markers, Xorw5 and Xorw6, and three microsatellite markers (Xwmc14, Xbarc97, and Xcfd175) were also completely linked to Pch1. On the other hand, Xwmc14, Xbarc97, and Xcfd175 showed recombination in the W7984 × Opata85 RIL population suggesting that recombination near Pch1 is reduced in the Coda/Brundage population. In a panel of 44 wheat varieties with known eyespot reactions, Xorw1, Xorw5, and Xorw6 were 100% accurate in predicting the presence or absence of Pch1 whereas Xwmc14, Xbarc97, and Xcfd175 were less effective. Thus, linkage mapping and a germplasm survey suggest that the STS markers identified here should be useful for indirect selection of Pch1.

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Acknowledgments

We would like to thank Dr. M. Isabel Vales for valuable discussions. Financial support from the Oregon State University Agricultural Research Foundation, Oregon Agricultural Experiment Station, the Oregon Wheat Commission, and the National Research Initiative of the US Department of Agriculture’s Cooperative State Research, Education, and Extension Service, Coordinate Agricultural Project (Wheat CAP) grant number 2006-55606-16629 are greatly appreciated.

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Authors and Affiliations

  1. Department of Crop and Soil Science, Oregon State University, 107 Crop Science Bldg, Corvallis, OR, 97331, USA

    Jeffrey M. Leonard, Christy J. W. Watson, Robert S. Zemetra & Oscar Riera-Lizarazu

  2. Department of Plant, Soil, and Entomological Sciences, University of Idaho, Moscow, ID, 83843, USA

    Arron H. Carter & Jennifer L. Hansen

  3. Department of Crop and Soil Sciences, Washington State University, Pullman, WA, 99164, USA

    Dipak K. Santra

  4. US Department of Agriculture, Agricultural Research Service, Washington State University, Pullman, WA, 99164, USA

    Kimberly G. Campbell

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  1. Jeffrey M. Leonard
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  2. Christy J. W. Watson
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  3. Arron H. Carter
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  4. Jennifer L. Hansen
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  8. Oscar Riera-Lizarazu
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Correspondence to Oscar Riera-Lizarazu.

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Communicated by F. Ordon.

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Leonard, J.M., Watson, C.J.W., Carter, A.H. et al. Identification of a candidate gene for the wheat endopeptidase Ep-D1 locus and two other STS markers linked to the eyespot resistance gene Pch1 . Theor Appl Genet 116, 261–270 (2008). https://doi.org/10.1007/s00122-007-0664-4

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  • DOI: https://doi.org/10.1007/s00122-007-0664-4

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