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A genome-wide analysis of differentiation between wild and domesticated Phaseolus vulgaris from Mesoamerica

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Abstract

Lack of introgression or divergent selection may be responsible for the maintenance of phenotypic differences between sympatric populations of crops and their wild progenitors. To distinguish between these hypotheses, amplified fragment length polymorphism markers were located on a molecular linkage map of Phaseolus vulgaris relative to genes for the domestication syndrome and other traits. Diversity for these same markers was then analyzed in two samples of wild and domesticated populations from Mesoamerica. Differentiation between wild and domesticated populations was significantly higher in parapatric and allopatric populations compared to sympatric populations. It was also significantly higher near genes for domestication compared to those away from these genes. Concurrently, the differences in genetic diversity between wild and domesticated populations were strongest around such genes. These data suggest that selection in the presence of introgression appears to be a major evolutionary factor maintaining the identity of wild and domesticated populations in sympatric situations. Furthermore, alleles from domesticated populations appear to have displaced alleles in sympatric wild populations, thus leading to a reduction in genetic diversity in such populations. These results also provide a possible experimental framework for assessing the long-term risk of transgene escape and the targeting of transgenes inside the genome to minimize the survival of these transgenes into wild populations following introduction by gene flow.

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Acknowledgements

This work was supported by the McKnight Foundation. RP was supported by CNR (Italy) for his stay in Davis and work at Ancona. We thank Guido Barbujani, Thomas Bataillon, Isabelle Olivieri, Cal Qualset, Delphine Sicard, Julianno Sambatti, Don Strong, Fabio Veronesi and Renaud Vitalis for their useful comments on an early version of the manuscript.

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Authors and Affiliations

  1. Department of Plant Sciences, Section of Crop and Ecosystem Sciences, University of California, Mailstop 1, 1 Shields Avenue, Davis, CA, 95616-8780, USA

    R. Papa & P. Gepts

  2. Dipartimento di Scienze degli Alimenti, Facoltà di Agraria, Universitá Politecnica delle Marche, Via Brecce Bianche, 60131, Ancona, Italy

    R. Papa

  3. Instituto Nacional de Investigaciones Forestales y Agropecuarias, Campo Experimental del Bajío, Apartado Postal 112, Guanajuato, CP 38000, Celaya, Mexico

    J. Acosta

  4. Instituto de Biología, Universidad Nacional Autónoma de México, Apartado Postal 70-233, 04510, México D.F., Mexico

    A. Delgado-Salinas

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  1. R. Papa
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  2. J. Acosta
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  3. A. Delgado-Salinas
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  4. P. Gepts
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Correspondence to P. Gepts.

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Communicated by F. J. Muehlbauer

This article is dedicated to the memory of Epimaki M. K. Koinange.

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Papa, R., Acosta, J., Delgado-Salinas, A. et al. A genome-wide analysis of differentiation between wild and domesticated Phaseolus vulgaris from Mesoamerica. Theor Appl Genet 111, 1147–1158 (2005). https://doi.org/10.1007/s00122-005-0045-9

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  • DOI: https://doi.org/10.1007/s00122-005-0045-9

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