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Mapping quantitative trait loci for yield, yield components and morphological traits in an advanced backcross population between Oryza rufipogon and the Oryza sativa cultivar Jefferson

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Abstract

An advanced backcross population between an accession of Oryza rufipogon (IRGC 105491) and the U.S. cultivar Jefferson (Oryza sativa ssp. japonica) was developed to identify quantitative trait loci (QTLs) for yield, yield components and morphological traits. The genetic linkage map generated for this population consisted of 153 SSR and RFLP markers with an average interval size of 10.3 cM. Thirteen traits were examined, nine of which were measured in multiple environments. Seventy-six QTLs above an experiment-wise significance threshold of P < 0.01 (corresponding to an interval mapping LOD > 3.6 or a composite interval mapping LOD > 3.9) were identified. For the traits measured in multiple environments, 47% of the QTLs were detected in at least two environments. The O. rufipogon allele was favorable for 53% of the yield and yield component QTLs, including loci for yield, grains per panicle, panicle length, and grain weight. Morphological traits related to the domestication process and/or weedy characteristics, including plant height, shattering, tiller type and awns, were found clustered on chromosomes 1 and 4. Comparisons to previous studies involving wild × cultivated crosses revealed O. rufipogon alleles with stable effects in multiple genetic backgrounds and environments, several of which have not been detected in studies between Oryza sativa cultivars, indicating potentially novel alleles from O. rufipogon. Some O. rufipogon-derived QTLs, however, were in similar regions as previously reported QTLs from Oryza sativa cultivars, providing evidence for conservation of these QTLs across the Oryza genus. In addition, several QTLs for grain weight, plant height, and flowering time were localized to putative homeologous regions in maize where QTLs for these traits have been previously reported, supporting the hypothesis of functional conservation of QTLs across the grasses.

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References

  • Alonso-Blanco C, Koornneef M (2000) Naturally occurring variation in Arabidopsis: an underexploited resource for plant genetics. Trends Plant Sci 5:22–29

    CAS  PubMed  Google Scholar 

  • Austin DF, Lee M (1996) Comparative mapping in F2:3 and F6:7 generations of quantitative trait loci for grain yield and yield components in maize. Theor Appl Genet 92:817–826

    Article  CAS  Google Scholar 

  • Austin DF, Lee M, Veldboom LR (2001) Genetic mapping in maize with hybrid progeny across testers and generations: plant height and flowering. Theor Appl Genet 102:163–176

    Article  CAS  Google Scholar 

  • Basten CJ, Weir BS, Zeng Z-B (1997) qtl cartographer: a reference manual and tutorial for QTL mapping. Department of Statistics, North Carolina State University, Raleigh, N.C.

  • Beadle GW (1939) Teosinte and the origin of maize. J Hered 30:245–247

    Google Scholar 

  • Bres-Patry C, Lorieux M, Clement G, Bangratz M, Ghesquiere A (2001) Heredity and genetic mapping of domestication-related traits in a temperate japonica weedy rice. Theor Appl Genet 102:118–126

    Article  CAS  Google Scholar 

  • Cai HW, Morishima H (2000) Genomic regions affecting seed shattering and seed dormancy in rice. Theor Appl Genet 100:840–846

    CAS  Google Scholar 

  • Cai HW, Morishima H (2002) QTL clusters reflect character associations in wild and cultivated rice. Theor Appl Genet 104:1217–1228

    Article  Google Scholar 

  • Causse MA, Fulton TM, Cho YG, Ahn SN, Chunwongse J, Wu K, Xiao J, Yu Z, Ronald PC, Harrington SE, Second G, McCouch SR, Tanksley SD (1994) Saturated molecular map of the rice genome based on an interspecific backcross population. Genetics 138:1251–1274

    CAS  PubMed  Google Scholar 

  • Chen X, Temnykh S, Xu Y, Cho YG, McCouch SR (1997) Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.). Theor Appl Genet 95:553–567

    Article  CAS  Google Scholar 

  • Cho YG, Eun MY, McCouch SR, Cae YA (1994) The semidwarf gene, sd-1, of rice (Oryza sativa L.): II. molecular mapping and marker-assisted selection. Theor Appl Genet 89:54–59

    CAS  Google Scholar 

  • Churchill GA, Doerge RW (1994) Empirical threshold value for quantitative trait mapping. Genetics 138:963–971

    CAS  PubMed  Google Scholar 

  • Davis GL, McMullen MD, Baysdorfer C, Musket T, Grant D, Staebell M, Xu G, Polacco M, Koster L, Melia HS, Houchins K, Chao S, Coe EH Jr (1999) A maize map standard with sequenced core markers, grass genome reference points and 932 expressed sequence tagged sites (ESTs) in a 1,736-locus map. Genetics 152:1137–1172

    CAS  PubMed  Google Scholar 

  • Doebley J, Stec A (1991) Genetic analysis of the morphological differences between maize and teosinte. Genetics 129:285–296

    CAS  PubMed  Google Scholar 

  • Doebley J, Stec A (1993) Inheritance of the morphological differences between maize and teosinte: comparison of results for two F2 populations. Genetics 134:559–570

    CAS  PubMed  Google Scholar 

  • Doebley J, Bacigalupo A, Stec A (1994) Inheritance of kernel weight in two maize-teosinte hybrid populations: implications for crop evolution. J Hered 85:191–195

    Google Scholar 

  • Doi K, Yoshimura A, Iwata N (1998) RFLP mapping and QTL analysis of heading date and pollen sterility using backcross populations between Oryza sativa L. and Oryza glaberrima Steud. Breed Sci 48:395–399

    CAS  Google Scholar 

  • Frary A, Nesbitt TC, Frary A, Grandillo S, van der Knaap E, Cong B, Lui J, Meller J, Elber R, Alpert KB, Tanksley SD (2000) fw2.2: a quantitative trait locus key to the evolution of tomato fruit size. Science 289:85–88

    Article  CAS  PubMed  Google Scholar 

  • Fridman E, Pleban T, Zamir D (2000) A recombination hotspot delimits a wild-species quantitative trait locus for tomato sugar content to 484 bp within an invertase gene. Proc Natl Acad Sci USA 97:4718–4723

    CAS  PubMed  Google Scholar 

  • Fukuta Y (1995) RFLP Mapping of a shattering-resistance gene in the mutant line, SR-1 induced from an indica rice variety, Nan-jing 11. Breed Sci 45:15–19

    CAS  Google Scholar 

  • Harlan JR (1976) Genetic resources in wild relatives of crops. Crop Sci 16:329–333

    Google Scholar 

  • Harlan JR, DeWet JMJ, Price EG (1973) Comparative evolution of cereals. Evolution 27:311–325

    Google Scholar 

  • Harushima Y, Kurata N, Yano M, Nagamura Y, Sasaki T, Minobe Y, Nakagahra M (1996) Detection of segregation distortion in an indica-japonica rice cross using a high-resolution molecular map. Theor Appl Genet 92:145–150

    Article  CAS  Google Scholar 

  • Harushima Y, Yano M, Shomura A, Sato M, Shimano T, Kuboki Y, Yamamoto T, Lin SY, Antonio BA, Parco A, Kajiya H, Huang N, Yamamoto K, Nagamura Y, Kurata N, Khush GS, Sasaki T (1998) A high-density rice genetic linkage map with 2,275 markers using a single F2 population. Genetics 148:479–494

    CAS  PubMed  Google Scholar 

  • Huang N, Courtois B, Khush GS, Lin H, Wang G, Wu P, Zheng K (1996) Association of quantitative trait loci for plant height with major dwarfing genes in rice. Heredity 77:130–137

    CAS  Google Scholar 

  • Huang N, Parco A, Mew T, Magapantay G, McCouch S, Guiderdoni E, Xu J, Subudhi P, Angelos ER, Khush GS (1997) RFLP mapping of isozymes, RAPD and QTLs for grain shape, brown planthopper resistance in a doubled haploid rice population. Mol Breed 3:105–113

    CAS  Google Scholar 

  • Khairallah MM, Bohn M, Jiang C, Deutsch JA, Jewell DC, Mihm JA, Melchinger AE, Gonzalez DLD, Hoisington DA (1998) Molecular mapping of QTLs for southwestern corn borer resistance, plant height and flowering in tropical maize. Plant Breed 117:309–318

    Google Scholar 

  • Koester RP, Sisco PH, Stuber CW (1993) Identification of quantitative trait loci controlling days to flowering and plant height in two near isogenic lines of maize. Crop Sci 33:1209–1216

    Google Scholar 

  • Kohn JR, Leyva N, Dossey R, Sobral B, Morishima H (1997) Quantitative trait locus analysis of trait variation among annual and perennial ecotypes of Oryza rufipogon. Int Rice Res Notes 22:4–5

    Google Scholar 

  • Koinange EMK, Singh SP, Gepts P (1996) Genetic control of the domestication syndrome in common bean. Crop Sci 36:1037–1045

    Google Scholar 

  • Lander R, Green P, Abrahamson L, Barlow A, Daley M, Lincoln S, Newburg L (1987) mapmaker: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    CAS  PubMed  Google Scholar 

  • Li JX, Yu SB, Xu CG, Tan YF, Gao YJ, Li XH, Zhang Q (2000) Analyzing quantitative trait loci for yield using a vegetatively replicated F2 population from a cross between the parents of an elite rice hybrid. Theor Appl Genet 101:248–254

    CAS  Google Scholar 

  • Li Z, Pinson SRM, Stansel JW, Park WD (1995) Identification of quantitative trait loci (QTL) for heading date and plant height in cultivated rice (Oryza sativa L.). Theor Appl Genet 91:374–381

    CAS  Google Scholar 

  • Li Z, Pinson SRM, Park WD, Paterson AH, Stansel JW (1997) Epistasis for three grain yield components in rice (Oryza sativa L.). Genetics 145:452–465

    Google Scholar 

  • Lin HX, Qian HR, Zhuang JY, Lu J, Min SK, Xiong ZM (1996) RFLP mapping of QTLs for yield and other related characters in rice. Int Rice Res Notes 21:17–19

    Google Scholar 

  • Lu C, Shen L, Tan Z, Xu Y, He P, Chen Y, Zhu L (1997) Comparative mapping of QTLs for agronomic traits of rice across environments by using a doubled-haploid population. Theor Appl Genet 94:145–150

    Article  Google Scholar 

  • Lyttle TW (1991) Segregation distorters. Annu Rev Genet 25:511–557

    CAS  PubMed  Google Scholar 

  • Maheswaran M, Huang N, Sreerangasamy SR, McCouch SR (2000) Mapping quantitative trait loci associated with days to flowering and photoperiod sensitivity in rice (Oryza sativa L.). Mol Breed 6:145–155

    Article  CAS  Google Scholar 

  • Manly KF, Olson JM (1999) Overview of QTL mapping software and introduction to map manager qt. Mammalian Genome 10:327–334

    Article  CAS  PubMed  Google Scholar 

  • McClung AM, Marchetti MA, Webb BD, Bollich CN (1997) Registration of 'Jefferson' Rice. Crop Sci 37:629–630

    Google Scholar 

  • McCouch SR, Doerge RW (1995) QTL mapping in rice. Trends Genet 11:482–487

    CAS  PubMed  Google Scholar 

  • McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman RW, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829

    CAS  Google Scholar 

  • McCouch SR, Thomson MJ, Septiningsih EM, Moncada P, Li J, Xiao J, Martinez C, Tohme J, Moelpojawiro S, Yuan LP, McClung A, Tai T, Guimaraes E, Moon HP, Ahn SN (2001) Wild QTLs for rice improvement. In: Rockwood W (ed) Proceedings of the Chandler symposium: rice research and production in the 21st century, June 15–17, 2000. IRRI, Philippines, pp 151–169

  • Moncada P, Martinez CP, Borrero J, Chatel M, Gauch H, Guimaraes E, Tohme J, McCouch SR (2001) Quantitative trait loci for yield and yield components in an Oryza sativa × Oryza rufipogon BC2F2 population evaluated in an upland environment. Theor Appl Genet 102:41–52

    Article  CAS  Google Scholar 

  • Monna L, Kitazawa N, Yoshino R, Suzuki J, Masuda H, Maehara Y, Tanji M, Sato M, Nasu S, Minobe Y (2002) Positional cloning of rice semidwarfing gene, sd-1: rice "green revolution gene" encodes a mutant enzyme involved in gibberellin synthesis. DNA Res 9:11–17

    Google Scholar 

  • Nagao S, Takahashi M (1963) Trial construction of twelve linkage groups in Japanese rice. Genetical studies on rice plant. 27. J Fac Agric Hokkaido Univ 53:72–130

    Google Scholar 

  • Nelson JC (1997) qgene: software for marker-based genomic analysis and breeding. Mol Breed 3:239–245

    CAS  Google Scholar 

  • Ogi Y, Kato H, Maruyama K, Saito A, Kikuchi F (1993) Identification of RFLP markers closely linked to the semidwarfing gene at the sd-1 locus in rice. Jpn J Breed 43:141–146

    CAS  Google Scholar 

  • Panaud O, Chen X, McCouch SR (1996) Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Mol Gen Genet 252:597–607

    Article  CAS  PubMed  Google Scholar 

  • Paterson AH, Lin YR, Li Z, Schertz KF, Doebley JF, Pinson SRM, Liu SC, Stansel JW, Irvine JE (1995) Convergent domestication of cereal crops by independent mutations at corresponding genetic loci. Science 269:1714–1718

    CAS  Google Scholar 

  • Payne CT, Zhang F, Lloyd AM (2000) GL3 encodes a bHLH protein that regulates trichome development in Arabidopsis through interaction with GL1 and TTG1. Genetics 156:1349–1362

    CAS  PubMed  Google Scholar 

  • Price AH, Young EM, Tomos AD (1997) Quantitative trait loci associated with stomatal conductance, leaf rolling and heading date mapped in upland rice (Oryza sativa). New Phytol 137:83–91

    Article  CAS  Google Scholar 

  • Ribaut JM, Hoisington DA, Deutsch JA, Jiang C, Gonzalez-de-Leon D (1996) Identification of quantitative trait loci under drought conditions in tropical maize. 1. Flowering parameters and the anthesis-silking interval. Theor Appl Genet 92:905–914

    CAS  Google Scholar 

  • Sano Y (1990) The genic nature of gamete eliminator in rice. Genetics 125:183–191

    CAS  PubMed  Google Scholar 

  • Sasaki A, Ashikari M, Ueguchi-Tanaka M, Itoh H, Nishimura A, Swapan D, Ishiyama K, Saito T, Kobayashi M, Khush GS, Kitano H, Matsuoka M (2002) A mutant gibberellin synthesis-gene in rice. Nature 416:701–702

    Google Scholar 

  • Schon CC, Melchinger AE, Boppenmaier J, Brunklaus-Jung E, Herrmann RG, Seitzer JF (1994) RFLP mapping in maize: quantitative trait loci affecting testcross performance of elite European flint lines. Crop Sci 34:378–389

    Google Scholar 

  • Takahashi Y, Shomura A, Sasaki T, Yano M (2001) Hd6, a rice quantitative trait locus involved in photoperiod sensitivity, encodes the alpha subunit of protein kinase CK2. Proc Natl Acad Sci USA 98:7922–7927

    Article  CAS  PubMed  Google Scholar 

  • Tan Z, Shen L, Kuang H, Lu C, Chen Y, Zhou K, Zhu L (1996) Identification of QTLs for lengths of the top internodes and other traits in rice and analysis of their genetic effects. Acta Genet Sin 23:439–446

    CAS  Google Scholar 

  • Tanksley SD, McCouch SR (1997) Seed banks and molecular maps: unlocking genetic potential from the wild. Science 277:1063–1066

    CAS  PubMed  Google Scholar 

  • Tanksley SD, Nelson JC (1996) Advanced backcross QTL analysis: a method for the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines. Theor Appl Genet 92:191–203

    Article  Google Scholar 

  • Temnykh S, Park W, Ayres N, Cartinhour S, Hauck N, Lipovich L, Cho YG, Ishii T, McCouch SR (2000) Mapping and genome organization of microsatellite sequences in rice (Oryza sativa L.). Theor Appl Genet 100:697–712

    CAS  Google Scholar 

  • Van Deynze AE, Sorrells ME, Park WD, Ayres NM, Fu HY, Cartinhour SW, Paul E, McCouch SR (1998) Anchor probes for comparative mapping of grass genera. Theor Appl Genet 97:356–369

    Article  Google Scholar 

  • Veldboom LR, Lee M (1994) Molecular-marker-facilitated studies of morphological traits in maize. II: Determination of QTLs for grain yield and yield components. Theor Appl Genet 89:451–458

    CAS  Google Scholar 

  • Veldboom LR, Lee M, Woodman WL (1994) Molecular marker-facilitated studies in an elite maize population: I. Linkage analysis and determination of QTLs for morphological traits. Theor Appl Genet 88:7–16

    CAS  Google Scholar 

  • Walker AR, Davison PA, Bolognesi WAC, James CM, Srinivasan N, Blundell TL, Esch JJ, Marks DM, Gray JC (1999) The TRANSPARENT TESTA GLABRA1 locus, which regulates trichome differentiation and anthocyanin biosynthesis in Arabidopsis, encodes a WD40 repeat protein. Plant Cell 11:1337–1349

    Article  CAS  PubMed  Google Scholar 

  • Wilson WA, Harrington SE, Woodman WL, Lee M, Sorrells ME, McCouch SR (1999) Inferences on the genome structure of progenitor maize through comparative analysis of rice, maize and the domesticated panicoids. Genetics 153:453–473

    CAS  PubMed  Google Scholar 

  • Wu P, Zhang G, Huang N (1996) Identification of QTLs controlling quantitative characters in rice using RFLP markers. Euphytica 89:349–354

    CAS  Google Scholar 

  • Xiao J, Li J, Yuan L, Tanksley SD (1995) Dominance is the major genetic basis of heterosis in rice as revealed by QTL analysis using molecular markers. Genetics 140:745–754

    CAS  PubMed  Google Scholar 

  • Xiao J, Li J, Yuan L, Tanksley SD (1996) Identification of QTLs affecting traits of agronomic importance in a recombinant inbred population derived from a subspecific cross. Theor Appl Genet 92:230–244

    Article  CAS  Google Scholar 

  • Xiao J, Li J, Grandillo S, Ahn SN, Yuan L, Tanksley SD, McCouch SR (1998) Identification of trait-improving quantitative trait loci alleles from a wild rice relative, Oryza rufipogon. Genetics 150:899–909

    CAS  PubMed  Google Scholar 

  • Xiong LZ, Liu KD, Dai XK, Xu CG, Zhang Q (1999) Identification of genetic factors controlling domestication-related traits of rice using an F2 population of a cross between Oryza sativa and O. rufipogon. Theor Appl Genet 98:243–251

    Article  CAS  Google Scholar 

  • Xu Y, Zhu L, Xiao J, Huang N, McCouch SR (1997) Chromosomal regions associated with segregation distortion of molecular markers in F2, backcross, doubled haploid, and recombinant inbred populations in rice (Oryza sativa L.). Mol Gen Genet 253:535–545

    Article  CAS  PubMed  Google Scholar 

  • Yan J, Zhu J, He C, Benmoussa M, Wu P (1998) Molecular dissection of developmental behavior of plant height in rice (Oryza sativa L.). Genetics 150:1257–1265

    CAS  PubMed  Google Scholar 

  • Yan J, Zhu J, He C, Benmoussa M, Wu P (1999) Molecular marker-assisted dissection of genotype × environment interaction for plant type traits in rice (Oryza sativa L.). Crop Sci 39:538–544

    Google Scholar 

  • Yano M (2001) Genetic and molecular dissection of naturally occurring variation. Curr Opin Plant Biol 4:130–135

    CAS  PubMed  Google Scholar 

  • Yano M, Sasaki T (1997) Genetic and molecular dissection of quantitative traits in rice. Plant Mol Biol 35:145–153

    CAS  PubMed  Google Scholar 

  • Yano M, Harushima Y, Nagamura Y, Kurata N, Minobe Y, Sasaki T (1997) Identification of quantitative trait loci controlling heading date in rice using a high-density linkage map. Theor Appl Genet 95:1025–1032

    Google Scholar 

  • Yano M, Katayose Y, Ashikari M, Yamanouchi U, Monna L, Fuse T, Baba T, Yamamoto K, Umehara Y, Nagamura Y, Sasaki T (2000) Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS. Plant Cell 12:2473–2483

    CAS  PubMed  Google Scholar 

  • Yu SB, Li JX, Xu CG, Tan YF, Gao YJ, Li XH, Zhang Q, Maroof MAS (1997) Importance of epistasis as the genetic basis of heterosis in an elite rice hybrid. Proc Natl Acad Sci USA 94:9226–9231

    CAS  PubMed  Google Scholar 

  • Zeng ZB (1994) Precision mapping of quantitative trait loci. Genetics 136:1457–1468

    CAS  PubMed  Google Scholar 

  • Zhuang JY, Lin HX, Lu J, Qian HR, Hittalmani S, Huang N, Zheng KL (1997) Analysis of QTL × environment interaction for yield components and plant height in rice. Theor Appl Genet 95:799–808

    Article  CAS  Google Scholar 

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Acknowledgements

We thank J. Mann of RiceTec for help with project organization and L. Zhao, B. Jordan, and M. Carpenter of RiceTec for technical assistance in the field. We are also grateful to F. Onishi at Cornell for technical assistance in the greenhouse, Julie Ho for critical review, and Lois Swales for manuscript formatting. This work was funded by the Rockefeller Foundation, the International Center for Tropical Agriculture (CIAT), the U.S. Department of Agriculture (USDA) Plant Genome Research Program (NRI grant no. 96-35300-3645), and through a graduate assistantship to M.J.T. provided by the Cornell Plant Cell and Molecular Biology Program (DOE/NSF/USDA Interagency Training Grant).

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Authors and Affiliations

  1. Department of Plant Breeding, 240 Emerson Hall, Cornell University, Ithaca, NY 14853-1901, USA

    M. J. Thomson & S. R. McCouch

  2. USDA-ARS CPGRU and Department of Agronomy and Range Science, One Shields Ave, University of California, Davis, CA 95616 USA

    T. H. Tai

  3. USDA/ARS, 1509 Aggie Dr, Beaumont, TX 77713, USA

    A. M. McClung & X-H. Lai

  4. RiceTec, P.O. Box 1305, Alvin, TX 77512, USA

    M. E. Hinga, K. B. Lobos & Y. Xu

  5. Centro Internacional de Agricultura Tropical, Apdo aereo 6713, Cali, Colombia

    C. P. Martinez

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  1. M. J. Thomson
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Thomson, M.J., Tai, T.H., McClung, A.M. et al. Mapping quantitative trait loci for yield, yield components and morphological traits in an advanced backcross population between Oryza rufipogon and the Oryza sativa cultivar Jefferson. Theor Appl Genet 107, 479–493 (2003). https://doi.org/10.1007/s00122-003-1270-8

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