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Intraspecific mitochondrial DNA variation in the Colorado potato beetle,Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

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Abstract

Restriction endonuclease analyses of mitochondrial DNA (mtDNA) were used to examine genetic variability and population structure inLeptinotarsa decemlineata (Say). A group of three enzymes, EcoRI, HpaI, and PstI, was used to reveal polymorphism both within and among some of the 10 populations tested, yielding 16 haplotypes in combination. The frequencies of these 16 haplotypes differed significantly across geographic regions, indicating some partitioning of mtDNA haplotypes. Estimates of mtDNA sequence divergence (δ) between haplotypes ranged from 0.016 to 0.135%, suggesting local differentiation of mtDNA in some populations. Analysis of these data suggests that Texas was colonized by more than one mtDNA lineage, most likely originating in Mexico. We hypothesize that a larger founder size for the initial introductions or high levels of variability in the parent population at the edge of the CPB expanding range led to the initial partitioning of haplotypes observed in samples from Texas.

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References

  • Afonso, J. M., Volz, A., Hernandez, M., Ruttkay, H., Gonzalez, M., Larruda, J. M., Cabrera, V. M., and Sperlich, D. (1990). Mitochondrial DNA variation and genetic structure in Old-World populations ofDrosophila subobscura.Mol. Biol. Evol. 7123.

    Google Scholar 

  • Avise, J. M. (1986). Mitochondrial DNA and the evolutionary genetics of higher animals.Phil. Trans. R. Soc. Lond. 312325.

    Google Scholar 

  • Avise, J. C., Arnold, J., Ball, R. M., Bermingham, E., Lamb, T., Neigel, J. E., Reeb, C. A., and Saunders, N. C. (1987). Intraspecific phylogeny: The mitochondrial DNA bridge between populations genetics and systematics.Annu. Rev. Ecol. Syst. 18489.

    Google Scholar 

  • Azeredo-Espin, A. M. L., Schroder, R. F. W., Huettel, M. D., and Sheppard, W. S. (1991). Mitochondrial DNA variation in geographic populations of Colorado potato beetle,Leptinotarsa decemlineata (Coleoptera: Chrysomelidae).Experientia 47483.

    Google Scholar 

  • Bentzen, P., Brown, G. C., and Leggett, W. C. (1989). Mitochondrial DNA polymorphism, population structure, and life history variation in American Shad (Alosa sapidissima).Can. J. Fish. Aquat. Sci. 461446.

    Google Scholar 

  • Birky, C. W., Maruyama, T., and Fuerst, P. (1983). An approach to population and evolutionary genetic theory for genes in mitochondria and chloroplasts and some results.Genetics 103513.

    Google Scholar 

  • Boyce, T. M., Zwick, M. E., and Aquadro, C. F. (1989). Mitochondrial DNA in the bark weevils: size, structure and heteroplasmy.Genetics 123825.

    Google Scholar 

  • Boyce, T. M., Zwick, M. E., and Aquadro, C. F. (1994). Mitochondrial DNA in the bark weevils: Phylogeny and evolution in thePissodes strobi group (Coleoptera: Curculionidae).Mol. Biol. Evol. 11(2):183.

    Google Scholar 

  • Brown, W. M. (1983). Evolution of animal mitochondrial DNA. In Nei, M., and Koehn, R. K. (eds.),Evolution of Genes and Proteins Sinauer Associates, Sunderland, MA, pp. 60–88.

    Google Scholar 

  • Brown, W. M., George, M., Jr., and Wilson, A. C. (1979). Rapid evolution of animal mitochondrial DNA.Proc. Natl. Acad. Sci. USA 761967.

    Google Scholar 

  • Caprio, M. A. (1987).Flight Initiation Behavior and Host Plant Attraction in the Colorado Potato Beetle Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) M.S. thesis, Michigan State University, East Lansing.

    Google Scholar 

  • Caprio, M. A., Miller, D., and Grafius, E. (1990). Marking adult Colorado potato beetles,Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) using paper labels.Great Lakes Entomol.2313.

    Google Scholar 

  • Casagrande, R. A. (1985). The “Iowa” potato beetle, its discovery and spread to potatoes.Bull. Entomol. Soc. Am. 3127.

    Google Scholar 

  • DeSalle, R., and Giddings, L. V. (1986). Discordance of nuclear and mitochondrial DNA of HawaiianDrosophila.Proc. Nat. Acad. Sci. USA 836902.

    Google Scholar 

  • DeSalle, R. L., Giddings, V., and Templeton, A. R. (1986). Mitochondrial DNA variability in natural populations of HawaiianDrosophila. I. Methods and levels of variability inD. silvestris andD. heteroneura populations.Heredity 5675.

    Google Scholar 

  • DeSalle, R. L., Freedman, T., Prager, E. M., and Wilson, A. C. (1987). Tempo and mode of sequence evolution in mitochondrial DNA of HawaiianDrosophila.J. Mol. Evol. 26157.

    Google Scholar 

  • Dively, G. P., Ellis, F. W., and Linduska, J. J. (1987). Colorado potato beetle resistance management practices. Pest Management Guidelines No. 40, Maryland Cooperative Extension Service.

  • Ferro, D. N., Logan, J. A., Voss, R., and Elkinton, J. S. (1985). Colorado potato beetle (Coleoptera: Chrysomelidae) temperature-dependent growth and feeding rates.Environ. Entomol. 14343.

    Google Scholar 

  • Forgash, A. J. (1981). Insecticide resistance of the Colorado potato beetle,Leptinotarsa decemlineata (Say).In Lashomb, J. H., and Casagrande, R. (eds.);Advances in Potato Pest Management Hutchison Ross, New York, pp. 34–46.

    Google Scholar 

  • Hale, L. R., and Singh, R. (1986). Extensive size variation and heteroplasmy in mitochondrial DNA among geographic populations ofD. melanogaster.Proc. Natl. Acad. Sci. USA 838813.

    Google Scholar 

  • Hale, L. R., and Singh, R. (1987). Mitochondrial DNA variation and genetic structure in populations ofDrosophila melanogaster.Mol. Biol. Evol. 4622.

    Google Scholar 

  • Hare, J. D. (1990). Ecology and management of the Colorado potato beetle.Annu. Rev. Entomol. 3581.

    Google Scholar 

  • Hare, J. D., and Kennedy, G. G. (1986). Genetic variation in plant-insect associations: Survival ofLeptinotarsa decemlineata populations onSolanum carolinense.Evolution 401031.

    Google Scholar 

  • Harrison, R. G., Rand, D. M., and Wheeler, W. C. (1985). Mitochondrial DNA size variation within individual crickets.Science 2281446.

    Google Scholar 

  • Horton, D. R., Capineara, J. L., and Chapman, P. L. (1988). Local differences in host use by two populations of the Colorado potato beetle.Ecology 69823.

    Google Scholar 

  • Hsiao, T. H. (1978). Host plant adaptations among geographic populations of the Colorado potato beetle.Entomol. Exp. Appl. 24437.

    Google Scholar 

  • Hsiao, T. H. (1981). Ecophysiological adaptation among geographic populations of the Colorado potato beetle in North America.In Lashomb, J. H., and Casagrande, R. (eds.),Advances in Potato Pest Management Hutchinson Ross, Stroudsburg, PA, pp. 69–85.

    Google Scholar 

  • Hsiao, T. H. (1985). Ecophysiological and genetic aspects of geographic variations of the Colorado potato.In Ferro, D. N., and Voss, R. H. (eds.),Proceedings, Symposium on the Colorado Potato Beetle XVIIth International Congress of Entomology, Mass. Agr. Exp. Stat. Res. Bull. 704, University of Massachusetts, Amherst, pp. 63–77.

    Google Scholar 

  • Hsiao, T. H., and Hsiao, C. (1983). Chromosomal analysis ofLeptinotarsa decemlineata andLabidomera species (Coleoptera: Chrysomelidae).Genetica 60139.

    Google Scholar 

  • Jacobson, J. W., and Hsiao, T. H. (1983). Isozyme variations between geographic populations of the Colorado potato beetle.Ann. Entomol. Soc. Am. 76162.

    Google Scholar 

  • Jacques, R., Jr. (1972).Taxonomic Revision of the Genus Leptinotarsa (Coleoptera: Chrysomelidae) of North America Ph.D. dissertation, Purdue University, West Lafayette, IN.

    Google Scholar 

  • Jacques, R., Jr. (1988). The potato beetle.Flora & Fauna Handbooks, No. 3, pp. 1–144.

  • Lashomb, J. H., and Ng, Y. (1984). Colonization by Colorado potato beetles,Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) in rotated and non-rotated potato fields.Environ. Entomol. 131352.

    Google Scholar 

  • Maniatis, T., Fritsch, E. F., and Sambrook, J. (1982).Molecular Cloning: A Laboratory Manual Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

    Google Scholar 

  • Moritz, C., Dowling, T. E., and Brown, W. M. (1987). Evolution of animal mitochondrial DNA: Relevance for population biology and systematics.Annu. Rev. Ecol. Syst. 18269.

    Google Scholar 

  • Nei, M., and Li, W.-H. (1979). Mathematical model for studying genetic variation in terms of restriction endonucleases.Proc. Natl. Acad. Sci. USA 765269.

    Google Scholar 

  • Rand, D. M., and Harrison, R. G. (1989). Molecular population genetics of mtDNA size variation in crickets.Genetics 121551.

    Google Scholar 

  • Reeb, C. A., and Avise, J. C. (1990). A genetic discontinuity in a continuously distributed species: Mitochondrial DNA in the American oyster,Crassostrea virginica.Genetics 124397.

    Google Scholar 

  • Sheppard, W. S., Rinderer, T. E., Mazzoli, J. A., Stelzer, J. A., and Shimanuki, H. (1991). Gene flow occurs between African- and European-derived honey bee populations in Argentina.Nature 349782.

    Google Scholar 

  • Solignac, M., Monnerot, M., and Mounlou, J. C. (1983). Mitochondrial heteroplasmy inDrosophila mauritiana.Proc. Natl. Acad. Sci. USA 806944.

    Google Scholar 

  • Solignac, M., Monnerot, M. and Mounolou, J. C. (1986). Mitochondrial DNA evolution in themelanogaster species subgroup ofDrosophila.J. Mol. Biol. 2331.

    Google Scholar 

  • Solignac, M., Genermont, M., Monnerot, M., and Mounolou, J. C. (1987).Drosophila mitochondrial genetics. Evolution of heteroplasmy through germ line cell division.Genetics 117687.

    Google Scholar 

  • Tauber, M. J., Tauber, C. A., Obrycki, J. J., Gollands, B., and Wright, R. J. (1988a). Geographical variation in response to photoperiod and temperature byLeptinotarsa decemlineata (Coleoptera: Chrysomelidae) during and after dormancy.Ann. Entomol. Soc. Am. 81764.

    Google Scholar 

  • Tauber, M. J., Tauber, C. A., Obrycki, J. J., Gollands, B., and Wright, R. J. (1988b). Geographical variation in response to photoperiod and temperature byLeptinotarsa decemlineata (Coleoptera: Chrysomelidae) after dormancy.Ann. Entomol. Soc. Am. 81764.

    Google Scholar 

  • Voss, R. H., and Ferro, D. N. (1985). Labelling the Colorado potato beetle with rubidium to study colonization and dispersal processes.Mass. Agr. Res. Stat. Bull. 70453.

    Google Scholar 

  • Voss, R. H., and Ferro, D. N. (1990a). Phenology of movement of Colorado potato beetle (Coleoptera: Chrysomelidae) adults by flight and walking in western Massachusetts.Environ. Entomol. 19117.

    Google Scholar 

  • Voss, R. H., and Ferro, D. N. (1990b). Ecology of migrating Colorado potato beetles (Coleoptera: Chrysomelidae) in western Massachusetts.Environ. Entomol. 19123.

    Google Scholar 

  • Wilson, A. C., Cann, R. L., Carr, S. M., George, M., Gyllenstein, U. B., Helmby Chowsky, K. M., Higuchi, R. G., Palumbi, S. R., Prager, E. M., Saye, R. D., and Stoneking, M. (1985). Mitochondrial DNA and two perspectives on evolutionary genetics.Biol. J. Linn. Soc. 26375.

    Google Scholar 

  • Wolstenholme, D. R. (1992). Animal mitochondrial DNA: Structure and evolution.In Wolstenholme, D. R., and Jeon Kwang, W. (eds.),Mitochondrial Genomes Academic Press, San Diego, CA, pp. 173–215.

    Google Scholar 

  • Wright, R. J. (1984). Evaluation of crop rotation for control of the Colorado potato beetle (Coleoptera: Chrysomelidae) in commercial potato fields on Long Island.J. Econ. Entomol. 771254.

    Google Scholar 

  • Zehnder, G. W., Sandall, L., Tisler, A. M., and Powers, T. O. (1992). Mitochondrial DNA diversity among seventeen geographic populations ofLeptinotarsa decemlineata (Coleoptera: Chrysomelidae).Ann. Entomol. Soc. Am. 85234.

    Google Scholar 

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Authors and Affiliations

  1. Departameto de Genética e Evoluĉão e Centro de Biologia Molecular e Eng. Genética, Universidade Estadual de Campinas, C.P. 6109- 13083-970, Campinas, SP, Brasil

    A. M. L. Azeredo-Espin

  2. Bee Research Laboratory, ARS-USDA, Building 476, Barc-East, 20705, Beltsville, Maryland

    A. M. L. Azeredo-Espin

  3. Insect Biocontrol Laboratory, ARS-USDA, Building 011-A, Barc-West, 20705, Beltsville, Maryland

    R. F. W. Schroder

  4. Center for Conservation Research and Training, 3050 Maile Way, Gilmore 310, University of Hawaii, 96822, Honolulu, Hawaii

    G. K. Roderick

  5. Department of Entomology, 166 FSHN Building, Washington State University, 99164, Pulman, Washington

    W. S. Sheppard

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  1. A. M. L. Azeredo-Espin
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  3. G. K. Roderick
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  4. W. S. Sheppard
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Azeredo-Espin, A.M.L., Schroder, R.F.W., Roderick, G.K. et al. Intraspecific mitochondrial DNA variation in the Colorado potato beetle,Leptinotarsa decemlineata (Coleoptera: Chrysomelidae). Biochem Genet 34, 253–268 (1996). https://doi.org/10.1007/PL00020576

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  • DOI: https://doi.org/10.1007/PL00020576

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