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“Tissue” transglutaminase and apoptosis

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Apoptosis

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 62))

Abstract

In this paper we discuss the role of “tissue” transglutaminase (tTG) in apoptosis. This enzyme by catalizing the Ca2+-dependent cross-linking of intracellular proteins leads to the formation of the SDS-insoluble protein scaffold in cells undergoing programmed cell death. These intracellular structures confer resistance to mechanical and chemical attack to the polipeptides involved in the linkages. tTG is induced during apoptosis, in fact, tTG mRNA is trascripted as a consequence of apoptosis induction. Overexpression of tTG in many cell lines enhances their susceptibility to apoptosis, indicating a pivotal role for tTG in this process. In keeping with these findings transfection of the human tTG complementary DNA in antisense orientation leads in a pronounced decrease of both spontaneous as well as induced apoptosis. Interestingly, the identification of the tTG substrate proteins in cells undergoing apoptosis has evidenced that many of the tTG proteins are also substrates of caspases.

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Abbreviations

DAG:

1,2-diacylglycerol

ICE:

interleukin 1β coverting enzyme

IL6:

interleukin 6

IP3 :

inositol-1,4,5-triphosphate

PGE2 :

prostaglandin E2

PKC:

Protein kinase C

PLC:

phospholipase C

TCR:

T cell receptor

TGFβ:

tumor growth factor

tTG:

“tissue” transglutaminase

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Authors and Affiliations

  1. Department of Biology, University of Rome “Tor Vergata”, Via della Ricerca Scientifica, 00133, Rome, Italy

    Francesco Autuori, Maria Grazia Farrace, Serafina Oliverio, Lucia Piredda & Mauro Piacentini

Authors
  1. Francesco Autuori
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  2. Maria Grazia Farrace
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  3. Serafina Oliverio
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  4. Lucia Piredda
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  5. Mauro Piacentini
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M. Al-Rubeai (Volume Editor)

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© 1998 Springer-Verlag Berlin Heidelberg

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Autuori, F., Farrace, M.G., Oliverio, S., Piredda, L., Piacentini, M. (1998). “Tissue” transglutaminase and apoptosis. In: Al-Rubeai, M. (eds) Apoptosis. Advances in Biochemical Engineering/Biotechnology, vol 62. Springer, Berlin, Heidelberg. https://doi.org/10.1007/BFb0102308

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  • DOI: https://doi.org/10.1007/BFb0102308

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-64153-7

  • Online ISBN: 978-3-540-69694-0

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