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Cytokinin signal transduction: Known simplicity and unknown complexity

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Chinese Science Bulletin

Abstract

Cytokinin plays a critical role in plant growth and development by regulating cell divisions and cell differentiation. Recent studies suggest that cytokinin signaling is presumably mediated by a two-component system analogous to those found in bacteria and fungi, which transduces an external signalvia a phosphorelay from the plasma membrane-anchored receptors to downstream effectors and regulators. Moreover, cytokinin signaling is highly interactive with other pathways, and many components of the pathway appear to be functionally redundant. Proper address of these questions will be crucial for our further understanding on this important network.

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References

  1. Davies, P. J., Plant Hormones: Physiology, Biochemistry, And Molecular Biology, Dordrecht: Kluwer Academic Press Boston, 1995.

    Google Scholar 

  2. Mok, D. W., Mok, M. C., Cytokinin metabolism and action, Annu. Rev. Plant Physiol. Plant Mol. Biol., 2001, 52: 89–118.

    Article  Google Scholar 

  3. Skoog, F., Miller, C. O., Chemical regulation of growth and organ formation in plant tissues culturedin vitro, Symp. Soc. Exp. Biol., 1957, 11: 118–131.

    Google Scholar 

  4. Wang, K. L., Li, H., Ecker, J. R., Ethylene biosynthesis and signaling networks, Plant Cell, 2002, 14: S131-S151.

    Google Scholar 

  5. Leyser, O., Molecular genetics of auxin signaling, Annu. Rev. Plant Physiol. Plant Mol. Biol., 2002, 53: 377–398.

    Google Scholar 

  6. Su, W., Howell, S.H., A single genetic locus,ckr1, definesArabidopsis mutants in which root growth is resistant to low concentrations of cytokinin, Plant Physiol., 1992, 99: 1569–1574.

    Article  Google Scholar 

  7. Vogel, J. P., Woeste, K. E., Theologis, A. et al., Recessive and dominant mutations in the ethylene biosynthetic geneACS5 ofArabidopsis confer cytokinin insensitivity and ethylene overproduction, respectively, Proc. Nat. Acad. Sci. USA, 1998, 95: 4766–4771.

    Article  Google Scholar 

  8. Chae, H. S., Faure, F., Kieber, J. J., Theeto1, eto2, andeto3 mutations and cytokinin treatment increase ethylene biosynthesis inArabidopsis by increasing the stability of ACS protein, Plant Cell, 2003, 15: 545–559.

    Article  Google Scholar 

  9. Abel, S., Blazquez, M., Dangl, J. et al.,Arabidopsis research 2000, Plant Cell, 2000, 12: 2302–2308.

    Article  Google Scholar 

  10. Kakimoto, T., CKI1, a histidine kinase homolog implicated in cytokinin signal transduction, Science, 1996, 274: 982–985.

    Article  Google Scholar 

  11. Sheen, J., Phosphorelay and transcription control in cytokinin signal transduction, Science, 2002, 296: 1650–1652.

    Article  Google Scholar 

  12. Hwang, I., Chen, H. C., Sheen, J., two-component signal transduction pathways inArabidopsis, Plant Physiol., 2002, 129: 500–515.

    Article  Google Scholar 

  13. Haberer, G., Kieber, J. J., Cytokinins: New insights into a classic phytohormone, Plant Physiol., 2002, 128: 354–362.

    Article  Google Scholar 

  14. Hutchison, C. E., Kieber, J. J., Cytokinin signaling inArabidopsis, Plant Cell, 2002, 14: S47-S59.

    Article  Google Scholar 

  15. D’Agostino, I. B., Kieber, J. J., Phosphorelay signal transduction: the emerging family of plant response regulators, Trends Biochem. Sci., 1999, 24: 452–456.

    Article  Google Scholar 

  16. Murashige, T., Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue culture, Physiol. Plant, 1962, 15: 473–497.

    Article  Google Scholar 

  17. Thomason, P., Kay, R., Eukaryotic signal transduction via histidine-aspartate phosphorelay, J. Cell Sci., 2000, 113: 3141–3150.

    Google Scholar 

  18. Yamada, H., Suzuki, T., Terada, K. et al., TheArabidopsis AHK4 histidine kinase is a cytokinin-binding receptor that transduces cytokinin signals across the membrane, Plant Cell Physiol., 2001, 42: 1017–1023.

    Article  Google Scholar 

  19. Pischke, M. S., Jones, G. L., Otsuga D. et al., AnArabidopsis histidine kinase is essential for megagametogenesis, Proc. Nat. Acad. Sci. USA, 2002, 99: 15800–15805.

    Article  Google Scholar 

  20. Hwang, I., Sheen, J., Two-component circuitry inArabidopsis cytokinin signal transduction, Nature, 2001, 413: 383–389.

    Article  Google Scholar 

  21. Inoue, T., Higuchi, M., Hashimoto, Y. et al., Identification of CRE1 as a cytokinin receptor fromArabidopsis, Nature, 2001, 409: 1060–1063.

    Article  Google Scholar 

  22. Urao, T., Yamaguchi-Shinozaki, K., Shinozaki, K., Two-component systems in plant signal transduction, Trends Plant Sci., 2000, 5: 67–74.

    Article  Google Scholar 

  23. Stock, J. B., Stock, A. M., Mottonen, J. M., Signal transduction in bacteria, Nature, 1990, 344: 395–400.

    Article  Google Scholar 

  24. Stock, A. M., Robinson, V. L., Goudreau, P. N., Two-component signal transduction, Annu. Rev. Biochem., 2000, 69: 183–215.

    Article  Google Scholar 

  25. Mahonen, A. P., Bonke, M., Kauppinen, L. et al., A novel two-component hybrid molecule regulates vascular morphogenesis of theArabidopsis root, Genes & Dev, 2000, 14: 2938–2943.

    Article  Google Scholar 

  26. Ueguchi, C., Koizumi, H., Suzuki, T. et al., Novel family of sensor histidine kinase genes inArabidopsis thaliana, Plant Cell Physiol., 2001, 42: 231–235.

    Article  Google Scholar 

  27. Franco-Zorrilla, J. M., Martin, A. C., Solano, R. et al., Mutations at CRE1 impair cytokinin-induced repression of phosphate starvation response inArabidopsis, Plant J., 2002, 32: 353–360.

    Article  Google Scholar 

  28. Banno, H., Ikeda, Y., Niu, Q. W. et al., Overexpression ofArabidopsis ESR1 induces initiation of shoot regeneration, Plant Cell, 2001, 13: 2609–2618.

    Article  Google Scholar 

  29. Sun, J., Niu, Q. W., Tarkowski, P. et al., TheArabidopsis AtIPT8/PGA22 gene encodes an isopentenyl transferase that is involved inde novo cytokinin biosynthesis, Plant Physiol., 2003, 131: 167–176.

    Article  Google Scholar 

  30. Zuo, J., Niu, Q. W., Frugis, G. et al., TheWUSCHEL gene promotes vegetative-to-embryonic transition inArabidopsis, Plant J., 2002, 30: 349–359.

    Article  Google Scholar 

  31. Takei, K., Sakakibara, H., Sugiyama, T., Identification of genes encoding adenylate isopentenyltransferase, a cytokinin biosynthesis enzyme, inArabidopsis thaliana, J. Biol. Chem., 2001, 276: 26405–26410.

    Article  Google Scholar 

  32. Kakimoto, T., Identification of plant cytokinin biosynthetic enzymes as dimethylallyl diphosphate: ATP/ADP isopentenyltransferases, Plant Cell Physiol., 2001, 42: 677–685.

    Article  Google Scholar 

  33. TheArabidopsis Genome Initiative, Analysis of the genome sequence of the flowering plantArabidopsis thaliana, Nature, 2000, 408: 796–815.

  34. Suzuki, T., Sakurai, K., Imamura, A. et al., Compilation and characterization of histidine-containing phosphotransmitters implicated in His-to-Asp phosphorelay in plants: AHP signal transducers ofArabidopsis thaliana, Biosci. Biotechnol. Biochem., 2000, 64: 2486–2489.

    Article  Google Scholar 

  35. Suzuki, T., Sakurai, K., Ueguchi, C. et al., Two types of putative nuclear factors that physically interact with histidine-containing phosphotransfer (Hpt) domains, signaling mediators in His-to-Asp phosphorelay, inArabidopsis thaliana, Plant Cell Physiol., 2001, 42: 37–45.

    Article  Google Scholar 

  36. Suzuki, T., Ishikawa, K., Yamashino, T. et al., AnArabidopsis histidine-containing phosphotransfer (HPt) factor implicated in phosphorelay signal transduction: overexpression ofAHP2 in plants results in hypersensitiveness to cytokinin, Plant Cell Physiol., 2002, 43: 123–129.

    Article  Google Scholar 

  37. Brandstatter, I., Kieber, J. J., Two genes with similarity to bacterial response regulators are rapidly and specifically induced by cytokinin inArabidopsis, Plant Cell, 1998, 10: 1009–1019.

    Article  Google Scholar 

  38. D’Agostino, I. B., Deruere, J., Kieber, J. J., Characterization of the response of theArabidopsis response regulator gene family to cytokinin, Plant Physiol., 2000, 124: 1706–1717.

    Article  Google Scholar 

  39. Imamura, A., Hanaki, N., Umeda, H. et al., Response regulators implicated in His-to-Asp phosphotransfer signaling inArabidopsis, Proc. Natl. Acad. Sci. USA, 1998, 95: 2691–2696.

    Article  Google Scholar 

  40. Sakakibara, H., Hayakawa, A., Deji, A. et al., His-Asp phosphotransfer possibly involved in the nitrogen signal transduction mediated by cytokinin in maize: molecular cloning of cDNAs for two-component regulatory factors and demonstration of phosphotransfer activityin vitro, Plant Mol. Biol., 1999, 41: 563–573.

    Article  Google Scholar 

  41. Sakai, H., Aoyama, T., Oka, A.,Arabidopsis ARR1 andARR2 response regulators operate as transcriptional activators, Plant J., 2000, 24: 703–711.

    Article  Google Scholar 

  42. Sakai, H., Honma, T., Aoyama, T. et al., ARR1, a transcription factor for genes immediately responsive to cytokinins, Science, 2001, 294: 1519–1521.

    Article  Google Scholar 

  43. Kiba, T., Yamada, H., Mizuno, T., Characterization of the ARR15 and ARR16 response regulators with special reference to the cytokinin signaling pathway mediated by the AHK4 histidine kinase in roots ofArabidopsis thaliana, Plant Cell Physiol., 2002, 43: 1059–1066.

    Article  Google Scholar 

  44. Xie, C., Zhang, J. S., Zhou, H. L. et al., Serine/threonine kinase activity in the putative histidine kinase-like ethylene receptor NTHK1 from tobacco, Plant J., 33: 385–393.

  45. Wang, W., Hall, A. E., O’Malley, R. et al., Canonical histidine kinase activity of the transmitter domain of the ETR1 ethylene receptor fromArabidopsis is not required for signal transmission, Proc. Nat. Acad. Sci. USA, 2003, 100: 352–357.

    Article  Google Scholar 

  46. Vogel, J. P., Schuerman, P., Woeste, K. et al., Isolation and characterization ofArabidopsis mutants defective in the induction of ethylene biosynthesis by cytokinin, Genetics, 1998, 149: 417–427.

    Google Scholar 

  47. Casson, S. A., Chilley, P. M., Topping, J. F. et al., ThePOLARIS gene ofArabidopsis encodes a predicted peptide required for correct root growth and leaf vascular patterning, Plant Cell, 2002, 14: 1705–1721.

    Article  Google Scholar 

  48. Sweere, U., Eichenberg, K., Lohrmann, J. et al., Interaction of the response regulator ARR4 with phytochrome B in modulating red light signaling, Science, 2001, 294: 1108–1111.

    Article  Google Scholar 

  49. Yamada, H., Hanaki, N., Imamura, A. et al., AnArabidopsis protein that interacts with the cytokinin-inducible response regulator, ARR4, implicated in the His-Asp phosphorylay signal transduction, FEBS Lett., 1998, 436: 76–80.

    Article  Google Scholar 

  50. Kircher, S., Kozma-Bognar, L., Kim, L. et al., Light quality-dependent nuclear import of the plant photoreceptors phytochrome A and B, Plant Cell, 1999, 11: 1445–1456.

    Article  Google Scholar 

  51. Chen, Y. F., Randlett, M. D., Findell, J. L. et al., Localization of the ethylene receptor ETR1 to the endoplasmic reticulum ofArabidopsis, J. Biol. Chem., 2002, 277: 19861–19866.

    Article  Google Scholar 

  52. Che, P., Gingerich, D. J., Lall, S. et al., Global and cytokinin-related gene expression changes during shoot development inArabidopsis, Plant Cell, 2002, 14: 2771–2785.

    Article  Google Scholar 

  53. Cary, A. J., Che, P., Howell, S. H., Developmental events and shoot apical meristem gene expression patterns during shoot development inArabidopsis thaliana, Plant J., 2003, 32: 867–877.

    Article  Google Scholar 

  54. Zuo, J., Niu, Q. W., Ikeda, Y. et al., Marker-free transformation: increasing transformation frequency by the use of regeneration-promoting genes, Curr. Opin. Biotech., 2002, 13: 173–180.

    Article  Google Scholar 

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Correspondence to Jianru Zuo.

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Zheng, B., Sun, J., Zhang, S. et al. Cytokinin signal transduction: Known simplicity and unknown complexity. Chin.Sci.Bull. 48, 1309–1315 (2003). https://doi.org/10.1007/BF03184168

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  • DOI: https://doi.org/10.1007/BF03184168

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