Abstract
Using the crystal structure of Despentapeptide (B26-B30) insulin (DPI as the search model, the crystal structure of DesB1-B2 Despentapeptide (B26-B30) insulin (DesB1-2 DPI) has been studied by the molecular replacement method. There is one DesB1-2 DPI molecule in each crystallographic asymmetric unit. The cross rotation function search and the translation function search show apparent peaks and thus determine the orientation and position of DesB1-2 DPI molecule in the cell respectively. The subsequent three-dimensional structural rebuilding and refinement of DesB1-2 DPI molecule confirm the results by molecular replacement method.
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Project supported by the Foundation of Chinese Academy of Sciences and the National Natural Science Foundation of China.
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Diao, J., Wan, Z., Chang, W. et al. Molecular replacement studies on crystal structure of DesB1-B2 Despentapeptide (B26-B30) insulin. Sci. China Ser. C.-Life Sci. 40, 524–530 (1997). https://doi.org/10.1007/BF03183592
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DOI: https://doi.org/10.1007/BF03183592