Abstract
Micrococcal nuclease has been covalently attached to CNBr-activated Sepharose 4B by coupling through three different enzyme functions: (a) amino groups; (b) carboxyl groups; and (c) tyrosyl or histidyl residues. On the basis of coupling yield and catalytic efficiency, Sepharose-(NH2) nuclease derivatives were chosen for further activity andstability studies. The activity of the insoluble enzyme has been evaluated with macromolecular (DNA) and small (synthetic nucleotide) substrates; with the latter the enzyme retains 70% of native enzyme activity. Good enhancement of enzyme stability in the 4–40°C range has been observed.
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Guisan, J.M., Ballesteros, A. Preparation of immobilized sepharose-micrococcal nuclease derivatives: activity and stability. Journal of Solid-Phase Biochemistry 4, 245–252 (1979). https://doi.org/10.1007/BF02998678
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DOI: https://doi.org/10.1007/BF02998678