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Long-term effect of tumor necrosis factor and gamma interferon in human pancreatic carcinoma cells

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Summary

The long-term cytotoxic/cytostatic effects of recombinant human tumor necrosis factor alpha (rhTNF) and gamma interferon (rhIFN-7) were studied in five human pancreatic carcinoma cell lines. The pancreatic tumor cell lines were heterogenous in their response to individual cytokines. During a 7-d incubation, MIA PaCa-2 cells were more sensitive to rhTNF than rhIFN-γ, whereas ASPC-1, T3M4, and COLO 357 cells were more sensitive to rhIFN-γ than rhTNF. PANC-1 cells were relatively insensitive to both cytokines. In a previous report, we demonstrated synergistic cytotoxic effects of rhTNF and rhIFN-γ during 48-h incubations in ASPC-1 and COLO 357 cells (Raitano et al,Pancreas, April, 1990). In this study, a 7-d treatment with both rhTNF and rhIFN-γ did not produce synergistic effects in any of the cell lines. However, a 24-h treatment with rhIFN-γ, followed by removal of the cytokine, markedly increased the long-term cytotoxic/cytostatic effects of rhTNF in ASPC-1, COLO 357, and T3M4 cells. In contrast, a similar pretreatment with rhTNF did not increase the long-term cytotoxic/cytostatic effects of rhIFN-γ in any of the cell lines. These data suggest that, in some human pancreatic carcinoma cell lines, rhIFN-γ may be especially useful in the long-term suppression of growth. Furthermore, brief pulses of rhIFN-γ may also be especially efficacious when followed by a subsequent prolonged exposure of cells to rhTNF.

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Abbreviations

BSA:

bovine serum albumin

FBS:

fetal bovine serum

HBSS:

Hank’s balanced salt solution

IFN-γ:

gamma interferon

PBS:

phosphate-buffered saline

rh:

recombinant human

TFN:

tumor necrosis factor

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Authors and Affiliations

  1. Departments of Microbiology and Immunology, Internal Medicine, Biochemistry, and the Arizona Cancer Center, University of Arizona School of Medicine, 85724, Tuscon, AZ

    Arthur B. Raitano, Philip Scuderi & Murray Korc

  2. Section of Endocrinology, Room 6411, Arizona Health Sciences Center, 1501 North Campbell Avenue, 85724, Tuscon, AZ

    Arthur B. Raitano, Philip Scuderi & Murray Korc

Authors
  1. Arthur B. Raitano
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  2. Philip Scuderi
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  3. Murray Korc
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Raitano, A.B., Scuderi, P. & Korc, M. Long-term effect of tumor necrosis factor and gamma interferon in human pancreatic carcinoma cells. Int J Pancreatol 6, 109–118 (1990). https://doi.org/10.1007/BF02933045

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  • DOI: https://doi.org/10.1007/BF02933045

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