Skip to main content
SpringerLink
Log in

Gene transfer by jet injection into differentiated tissues of living animals and in organ culture

  • Protocol
  • Published:
Molecular Biotechnology Aims and scope Submit manuscript

Abstract

Jet injection can be used to introduce genes into the cells of differentiated tissues of living animals and organ cultures. When a solution of plasmid DNA is jet injected into a selected tissue or organ, cells lying in or near the path of the jet injection are transfected with the DNA and the introduced gene(s) are expressed. Since there is minimal morbidity from each jet injection, multiple injections can be performed at the same or nearby sites. Both mRNA and protein expression from transfected genes can be quantitated using standard methods. In addition, the technique is an efficient means of DNA immunization. Methodology for using jet injection to transfer plasmid DNA into the cells of skin, fat, mammary gland, and muscle are described.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Furth, P. A., Shamay, A., Wall, R. J., and Hennighausen, L. (1992) Gene transfer into somatic tissue by jet injection.Anal. Biochem. 205, 365–368.

    Article  CAS  Google Scholar 

  2. Brandsma, J., Yang, Z.-H., Barthold, S. W., and Johnson, E. A. (1991) Use of a rapid, effficient inoculation method to induce papillomas by cottontail rabbit papillomavirus DNA shows that the E7 gene is required.Proc. Natl. Acad. Sci. USA 88, 4816–4820.

    Article  CAS  Google Scholar 

  3. Wolff, J. A., Williams, P., Acsadi, G., Jiao, S., Jani, A., and Chong, W. (1990) Direct gene transfer into mouse muscle in vivo.Science 247, 1465–1468.

    Article  CAS  Google Scholar 

  4. Sikes, M. L., O’Malley, B. W., Jr., Finegold, M. J., and Ledley, F. D. (1994)In vivo gene transfer into rabbit thyroid follicular cells by direct DNA injection.Hum. Gene Ther. 6, 837–844.

    Article  Google Scholar 

  5. Nabel, E. G., Plautz, G., and Nabel, G. J. (1990) Site-specific gene expression in vivo by direct transfer into the arterial wall.Science 249, 1285–1288.

    Article  CAS  Google Scholar 

  6. Nabel, G. J., Nabel, E. G., Yang, Z., Fox, B. A., Plautz, G. E., Gao, X., Huang, L., Shu, S., Gordon, D., and Chang, A. E. (1993) Direct gene transfer with DNA-liposome complexes in melanoma: Expression, biologic activity, and lack of toxicity in humans.Proc. Natl. Acad. Sci. USA 90, 11, 307-11,311.

    Article  Google Scholar 

  7. Alton, E. W., Middleton, P. G., Caplen, N. J., Smith, S. N., Steel, D. M., Munkonge, F. M., Jeffery, P. K., Geddes, D. M., Hart, S. L., and Williamson, R. (1993) Non-invasive liposome-mediated gene delivery can correct the ion transport defect in cystic fibrosis mutant mice (published erratum appears inNat. Genet. [1993]5, 312.).Nat. Genet. 5, 135–142.

    Article  CAS  Google Scholar 

  8. Perales, J. C., Ferkol, T., Beegen, H., Ratnoff, O. D., and Hanson, R. W. (1994) Gene transferin vivo: Sustained expression and regulation of genes introduced into the liver by receptor-targeted uptake.Proc. Natl. Acad. Sci. USA 91, 4086–4090.

    Article  CAS  Google Scholar 

  9. Stankovics, J., Crane, A. M., Andrews, E., Wu, C. H., Wu, G. Y., and Ledley, F. D. (1994) Over-expression of human methylmalonyl CoA mutase in mice after in vivo gene transfer with asialoglycoprotein/polylysine/DNA complexes.Hum. Gene Ther. 5, 1095–1104.

    Article  CAS  Google Scholar 

  10. Yang, N.-S., Burkholder, J. K., Roberts, B., Martinell, B., and McCabe, D. (1990) In vivo and in vitro gene transfer to mammalian somatic cells by particle bombardment.Proc. Natl. Acad. Sci. USA 87, 9568–9572.

    Article  CAS  Google Scholar 

  11. Williams, R. S., Johnson, S. A., Riedy, M., DeVit, M. J., McElligott, S. G., and Sanford, J. C. (1991) Introduction of foreign genes into tissues of living mice by DNA-coated microprojectiles.Proc. Natl. Acad. Sci. USA 88, 2726–2730.

    Article  CAS  Google Scholar 

  12. Furth, P. A., Shsmay, A., and Hennighausen, L. (1995) Gene transfer into mammalian cells by jet injection.Hybridoma 14, 149–152.

    CAS  Google Scholar 

  13. Kerr, D., Furth, P. A., and Wall, R. (1995) Expression of jet-infected plasmid DNA in the ovine mammary gland and in lymph nodes draining the injected site. Unpublished data.

  14. Honig, M. C. and Hume, R. I. (1989) Dil and DiO: versatile flourescent dyes for neuronal labelling and pathway tracing.TINS 12, 333–341.

    CAS  Google Scholar 

  15. Brandsma, J. L., Yang, Z.-H., DiMaio, D., Barthold, S. W., Johnson, E., and Xiao, W. (1992) The putative E5 open reading frame of cottontail rabbit papillomavirus is dispensible for papilloma formation in domestic rabbits.J. Virol. 66, 6204–6207.

    CAS  Google Scholar 

  16. Defeo-Jones, D., Vuocolo, G. A., Haskell, K. M., Hanobik, M. G., Kiefer, D. M., McAvoy, E. M., Ivey-Hoyle, M., Brandsma, J., Oliff, A., and Jones, R. E. (1993) Papillomavirus E7 protein binding to the retinoblastoma protein is not required for viral induction of warts.J. Virol. 67, 716–725.

    CAS  Google Scholar 

  17. Brandsma, J. and Xiao, W. (1993) Infectious virus replication in papillomas induced by molecularly cloned cottontail rabbit papillomavirus DNA.J. Virol. 67, 567–571.

    CAS  Google Scholar 

  18. Wu, X., Xiao, W., and Brandsma, J. L. (1994) Papilloma formation by cottontail rabbit papillomavirus requires El and E2 regulatory genes in addition to E6 and E7 transforming genes.J. Virol. 68, 6097–6102.

    CAS  Google Scholar 

  19. Ulmer, J. B., Donnelly, J. J., Parker, S. E., Rhodes, G. H., Feigner, P. L., Dwarki, V. J., Gromkowski, S. H., Deck, R. R., DeWitt, C. M., Friedman, A., Hawe, L. A., Leander, K. R., Martinez, D., Perry, H. C., Shiver, J. W., Montgomery, D. L., and Liu, M. A. (1993) Heterologous protection against influenza by injection of DNA encoding a viral protein.Science 259, 1745–1749.

    Article  CAS  Google Scholar 

  20. Fynan, E. F., Webster, R. G., Fuller, D. H., Haynes, J. R., Santoro, J. C., and Robinson, H. L. (1993) DNA vaccines: Protective immunizations by parental, mucosal, and gene-gun inoculations.Proc. Natl. Acad. Sci. USA 90, 11, 478-11,482.

    Article  Google Scholar 

  21. Tang, D., DeVit, M., and Johnston, S. A. (1992) Genetic immunization is a simple method for eliciting an immune response.Nature 356, 152–154.

    Article  CAS  Google Scholar 

  22. Eisenbraun, M. D., Fuller, D. H., and Haynes, J. R. (1993) Examination of parameters affecting the elicitation of humoral immune responses by particle bombardment-mediated genetic immunization.DNA Cell Biol. 12, 791–797.

    Article  CAS  Google Scholar 

  23. Furth, P. A., Hennighausen, L., Baker, C., Beatty, B., and Woychick, R. (1991) The variability in activity of the universally expressed human cytomegalovirus immediate early gene 1 enhancer/promoter in transgenic mice.Nucleic Acids Res. 19, 6205–6208.

    Article  CAS  Google Scholar 

  24. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989)Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.

    Google Scholar 

  25. Furth, P. A., St. Onge, L., Boger, H., Gruss, P., Gossen, M., Bujard, H., and Hennighausen, L. (1994) Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter.Proc. Natl. Acad. Sci. USA 91, 9302–9306.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Infectious Diseases, Department of Medicine, University of Maryland School of Medicine and the Baltimore Veterans Administration Medical Center, 10 North Greene Street, 21201, Baltimore, MD

    Priscilla A. Furth

  2. AltraBio Inc., Arden Hills, MN

    David Kerr

  3. Gene Evaluation and Mapping Laboratory, USDA-ARS, Beltsville, MD

    Robert Wall

Authors
  1. Priscilla A. Furth
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. David Kerr
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Robert Wall
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Furth, P.A., Kerr, D. & Wall, R. Gene transfer by jet injection into differentiated tissues of living animals and in organ culture. Mol Biotechnol 4, 121–127 (1995). https://doi.org/10.1007/BF02921606

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02921606

Index Entries

Search

Navigation