Abstract
Improvement have been made in a simplified procedure we previously reported (J.Mol.Catal. (1986),38,227 for the activation of tosyl chloride of supports possessing primary hydroxyl groups. The method is simple, can be completed in less than 90 min, yields a broad range of activation degrees, and, since it involves no toxic reagents, may be used for preparing immobilized enzymes to be utilized in food manufacturing and processing. The immobilization ofStophylococcal Nuclease has been carried out by this method. The insolubilized derivatives are more active than the native enzyme in the hydrolysis of DNA. The thermal stability of nuclease derivatives is greater than that of the native enzyme. These derivatives remain active at 50°C, and the native enzyme, 39°C. The insolubilized nuclease is more stable against organic solvents such as, dimethylsulfoxide (DMSO) or tetrahydroduran (THF) than the native enzyme.
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Alcantara, A., Ballesteros, A. & Sinisterra, J.V. New methodology for tosylation of hydroxylic supports as exemplified by the immobilization of micrococcal endonuclease on agarose. Appl Biochem Biotechnol 26, 297–310 (1990). https://doi.org/10.1007/BF02921508
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DOI: https://doi.org/10.1007/BF02921508