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Glomerular and vascular injury in mice following immunization with heterologous and autologous fibronectin

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Virchows Archiv B

Summary

Random bred Swiss-Webster mice were immunized with either autologous (MFN) or heterologous guinea pig (GPFN) denatured serum fibronectin. Immunofluorescent, light, and electron microscopic examination of renal tissues demonstrated glomerular changes, consisting primarily of endothelial and mesangial cell hypertrophy with expansion of the mesangial matrix. Evagination of mesangial cytoplasm into capillary lumens and balloon-like structures were characteristic of affected glomeruli. The histopathologic alterations were present in varying degrees of severity in all fibronectin treated animals, with slightly more extensive glomerular proliferation seen in animals immunized with heterologous (GPFN) fibronectin as compared to mice immunized with autologous (MFN) protein. Perivascular mononuclear cell infiltration with edematous changes in medial smooth muscle cells occured in renal vessels. The vasculature of the liver and lung also showed mononuclear cell infiltrates in the adventitia. These studies lead us to conclude that an immune response to either heterologous or autologous denatured serum fibronectin can induce glomerular sclerotic changes, cellular hyperplasia, and vascular injury.

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Authors and Affiliations

  1. Department of Pathology and the Immunobiology Research Center, University of Wisconsin Medical School, 53706, Madison, Wisconsin, USA

    Joanne E. Murphy-Ullrich, Terry D. Oberley & Deane F. Mosher

  2. Department of Medicine and the Immunobiology Research Center, University of Wisconsin Medical School, 53706, Madison, Wisconsin, USA

    Joanne E. Murphy-Ullrich, Terry D. Oberley & Deane F. Mosher

Authors
  1. Joanne E. Murphy-Ullrich
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  2. Terry D. Oberley
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  3. Deane F. Mosher
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Additional information

This work was supported by a Basil O’Connor Starter Research Grant from the March of Dimes and by a grant from the Dane County Medical Society to T.D. Oberley. J.E. Murphy-Ullrich was supported by a pre-doctoral traineeship, grant CA 09106. Support was also recieved from grant CHL-24885 to Deane F. Mosher. This work was done during the tenure of an Established Investigatorship from the Wisconsin Heart Association and its Wisconsin affiliate to DFM.

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Murphy-Ullrich, J.E., Oberley, T.D. & Mosher, D.F. Glomerular and vascular injury in mice following immunization with heterologous and autologous fibronectin. Virchows Archiv B Cell Pathol 39, 305–321 (1982). https://doi.org/10.1007/BF02892857

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  • DOI: https://doi.org/10.1007/BF02892857

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