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Effects ofEGb 761 on fatty acid reincorporation during reperfusion following ischemia in the brain of the awake gerbil

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Molecular and Chemical Neuropathology

Abstract

Transient cerebral ischemia (5 min) releases unesterified fatty acids from membrane phospholipids, increasing brain concentrations of fatty acids for up to 1 h following reperfusion. To understand the reported anti-ischemic effect ofGinkgo biloba extract (EGb 761), we monitored its effect on brain fatty acid reincorporation in a gerbilstroke model. Both common carotid arteries in awake gerbils were occluded for 5 min, followed by 5 min of reperfusion. Animals were infused intravenously with labeled arachidonic (AA) or palmitic acid (Pam), and rates of incorporation of unlabeled fatty acid from the brain acyl-CoA pool were calculated by the model of Robinson et al. (1992), using quantitative autoradiography and biochemical analysis of brain acyl-CoA. Animals were treated for 14 d with 50 or 150 mg/kg/dEGb 761 or vehicle. Ischemia-reperfusion had no effect on the rate of unlabeled Pam incorporation into brain phospholipids from palmitoyl-CoA; this rate also was unaffected byEGb 761. In contrast, ischemia-reperfusion increased the rate of incorporation of unlabeled AA from brain arachidonoyl-CoA by a factor of 2.3–3.3 compared with the control rate; this factor was further augmented to 3.6–5.0 by pretreatment withEGb 761. There is selective reincorporation of AA compared with Pam into brain phospholipids following ischemia.EGb 761 further accelerates AA reincorporation, potentially reducing neurotoxic effects of prolonged exposure of brain to high concentrations of AA and its metabolites.

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Abbreviations

EGb 761 :

Ginkgo biloba extract

[3H]AA:

[3H]arachidonic acid

[3H]Pam:

[3H]palmitic acid

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Authors and Affiliations

  1. Laboratory of Neurosciences, National Institutes on Aging, National Institutes of Health, 20892-1582, Bethesda, MD

    Olivier Rabin, Eric Grange, Michael C. J. Chang, Stanley I. Rapoport & A. David Purdon

  2. Institut Henri Beaufour, 35, rue Spontini, 75116, Paris, France

    Katy Drieu

Authors
  1. Olivier Rabin
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  2. Katy Drieu
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  3. Eric Grange
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  4. Michael C. J. Chang
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  5. Stanley I. Rapoport
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  6. A. David Purdon
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Rabin, O., Drieu, K., Grange, E. et al. Effects ofEGb 761 on fatty acid reincorporation during reperfusion following ischemia in the brain of the awake gerbil. Molecular and Chemical Neuropathology 34, 79–101 (1998). https://doi.org/10.1007/BF02815137

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  • DOI: https://doi.org/10.1007/BF02815137

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