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Development of long-lifetime metal-ligand probes for biophysics and cellular imaging

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Abstract

Metal-ligand complexes containing ruthenium, osmium, or rhenium display a high photostability, with polarized emission and decay times from 100 ns to 100 Μs. Such probes have considerable potential in biophysics, clinical chemistry, and fluorescence microscopy. In this review we sumrecent developments from this laboratory on the spectral properties of conjugatable metalligand complexes. We also suggest how improved probes can be developed based on the selection of organic ligands.

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Abbreviations

MLC:

metal-ligand complexes

FLIM:

fluorescence lifetime imaging microscopy

HSA:

human serum albumin

bpy:

2,2′-bipyridine

dcbpy:

4,4′-dicarboxy-2,2′-bipyridine

MLCT:

metal-ligand charge transfer

MPE:

multiphoton excitation

FRET:

fluorescence resonance energy transfer

LED:

light-emitting diode

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Authors and Affiliations

  1. Center for Fluorescence Spectroscopy and Medical Biotechnology Center, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 725 West Lombard Street, 21201, Baltimore, Maryland

    Joseph R. Lakowicz, Ewald Terpetschnig, Zakir Murtaza & Henryk Szmacinski

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  1. Joseph R. Lakowicz
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  2. Ewald Terpetschnig
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  3. Zakir Murtaza
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  4. Henryk Szmacinski
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Lakowicz, J.R., Terpetschnig, E., Murtaza, Z. et al. Development of long-lifetime metal-ligand probes for biophysics and cellular imaging. J Fluoresc 7, 17–25 (1997). https://doi.org/10.1007/BF02764573

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  • DOI: https://doi.org/10.1007/BF02764573

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