Summary
A C8 reversed-phase open tubular capillary column is dynamically modified with cetylpyridinium chloride to provide anion-exchange sites. Sodium salicylate is used as the eluent at the micromolar concentration range. The low capacity of the dynamically modified column allows the elution of common anions with k′ values similar to those in conventional ion chromatography. The reduced salicylate concentration allows good detectability in the indirect fluorescence mode, even though the baseline noise increased because the fluorescence intensity is lower. The noise level is equivalent to one picogram of non-fluorescing anions.
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References
M. Novotny, Ed., “Microcolumn Separations”, Elsevier, Amsterdam, 1985.
H. G. Menet, P. C. Gareil, R. H. Rosset, Anal. Chem.56, 1770 (1984).
J. H. Knox, M. T. Gilbert, J. Chromatogr.186, 405, (1979).
J. W. Jorgenson, E. J. Guthrie, J. Chromatogr.255, 355 (1983).
H. Small, in “Trace Analysis”,J. F. Lawrence, Ed., Academic Press, New York, Vol. 1., 1982, p. 267.
J. S. Fritz, D. T. Gjerde, C. Pohlandt, “Ion Chromatography”, Hüthig, New York, 1982.
H. Small, Anal. Chem.55, 235A (1983).
H. Small, T. E. Miller, Anal. Chem.54, 462 (1982).
D. R. Bobbit, E. S. Yeung, Anal. Chem.56, 1577 (1984).
S. I. Mho, E. S. Yeung, Anal. Chem.57, 2253 (1985).
T. Takeuchi, E. S. Yeung, J. Chromatogr.366, 145 (1986).
T. Takeuchi, E. S. Yeung, J. Chromatogr.370, 83 (1986).
M. J. Sepaniak, E. S. Yeung, J. Chromatogr.190, 377 (1980).
R. M. Cassidy, S. Elchuk, Anal. Chem.54, 1558 (1982).
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Pfeffer, W.D., Takeuchi, T. & Yeung, E.S. Anion chromatography in open tubular capillary columns with indirect fluorometric detection. Chromatographia 24, 123–126 (1987). https://doi.org/10.1007/BF02688474
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DOI: https://doi.org/10.1007/BF02688474