Skip to main content
SpringerLink
Log in

Complement-dependent induction of DNA synthesis and cell proliferation in human liver connective tissue cells in vitro

  • Infectious Disease/Cellular Pathology
  • Published:
In Vitro Cellular & Developmental Biology - Animal Aims and scope Submit manuscript

Summary

Liver connective tissue cells (LCTC) isolated from patients with fibrotic livers have morphological and biochemical characteristics of myofibroblasts. We have examined the proliferation of LCTC derived from normal livers and from livers with fibrosis of different etiologies, as well as proliferation of skin fibroblasts. We have compared proliferation rates in the presence of fresh human serum and heat-inactivated serum. While skin fibroblast and LCTC from normal liver showed no difference, proliferation of LCTC from fibrotic livers was markedly decreased in the presence of heat-inactivated serum. We demonstrate that the native complement component C1 is a factor involved in the induction of DNA synthesis and proliferation of LCTC isolated from fibrotic livers. We propose that native C1, acting probably in cooperation with other growth factors, is involved in the expansion of connective tissue cells during the development of liver fibrosis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Andrade, Z. Hepatic schistosomiasis. Morphological aspects. In: Popper, H.; Schaffner, F., eds. Progress in liver disease. Vol. 2. New York: Grune & Stratton; 1965:228–242.

    Google Scholar 

  2. Bordin, S.; Page, R. C. Role of platelet factors and serum complement in growth of fibroblasts with high affinity C1q complement receptors. In Vitro Cell. Dev. Biol. 24:719–726; 1988.

    Article  PubMed  CAS  Google Scholar 

  3. Bordin, S.; Page, R. C.; Narayanan, A. S. Heterogeneity of normal adult fibroblasts: isolation and characterization of one phenotype. Science 223:171–173; 1984.

    Article  PubMed  CAS  Google Scholar 

  4. Borojevic, R.; Vinhas, S. A.; Monteiro, A. N. A., et al. Liver connective tissue cells isolated from human schistosomal fibrosis or alcoholic cirrhosis represent a modified phenotype of smooth muscle cells. Biol. Cell 53:231–238; 1985.

    PubMed  CAS  Google Scholar 

  5. Bunchet, E.; Wake, K. Role of mesenchymal cell populations in porcine serum-induced rat liver fibrosis. Hepatology 16:1452–1473; 1992.

    Article  Google Scholar 

  6. Gabbiani, G.; Majno, G.; Ryan, G. B. The fibroblast as a contractile cell: the myofibroblast. In: Kulonen, E.; Pikkarainen, J. eds. Biology of the fibroblast. New York: Academic Press; 1983:139–154.

    Google Scholar 

  7. Galambos, J. T.; Hollingsworth, M. A., Jr.; Falek, A., et al. The rate of synthesis of glycosaminoglycans and collagen by fibroblasts cultured from adult human liver biopsies. J. Clin. Invest. 60:107–114; 1977.

    Article  PubMed  CAS  Google Scholar 

  8. Grimaud, J. A.; Borojevic, R. Myofibroblasts in hepatic schistosomal fibrosis. Experientia 33:890–892; 1977.

    Article  PubMed  CAS  Google Scholar 

  9. Grimaud, J. A.; Borojevic, R. Chronic human schistosomiasis mansoni: pathology of the Disse’s space. Lab. Invest. 36:268–273; 1977.

    PubMed  CAS  Google Scholar 

  10. Grimaud, J. A.; Borojevic, R. Portal fibrosis: intrahepatic portal vein pathology in chronic schistosomiasis mansoni. J. Submicroscop. Cytol. 18:783–793; 1986.

    CAS  Google Scholar 

  11. Korotzer, T. I.; Clagett, J. A.; Kolb, W. F., et al. Complement dependent induction of DNA synthesis and proliferation of human diploid fibroblasts. J. Cell Physiol. 105:503–512; 1980.

    Article  PubMed  CAS  Google Scholar 

  12. Miao, S.; Bao-En, W.; Annoni, G., et al. Two rat models of hepatic fibrosis. Lab. Invest. 63:467–475; 1990.

    Google Scholar 

  13. Monteiro, A. N. A.; Borojevic, R. In vitro formation of fibrous septa by liver connective tissue cells. In Vitro Cell. Dev. Biol. 23:10–14; 1987.

    Article  PubMed  CAS  Google Scholar 

  14. Monteiro, A. N. A.; Borojevic, R. Interaction of human liver connective tissue cells, skin fibroblasts and smooth muscle cells with collagen gels. Hepatology 7:665–671; 1987.

    Article  PubMed  CAS  Google Scholar 

  15. Narayanan, A. S.; Page, R. C. Connective tissue of the periodontium; a summary of current work. Coll. Rel. Res. 3:33–64; 1983.

    CAS  Google Scholar 

  16. Rudolph, R.; McClure, J. W.; Woodward, M. Contractile fibroblasts in chronic alcoholic cirrhosis. Gastroenterology 76:704–709; 1979.

    PubMed  CAS  Google Scholar 

  17. Sama, S. K.; Bhargava, S.; Gopi-Nath, N., et al. Non-cirrhotic portal fibrosis. Am. J. Med. 51:161–169; 1971.

    Article  Google Scholar 

  18. Schaffner, F.; Popper, H. Capillarization of hepatic sinusoids. Gastroenterology 44:239–242; 1963.

    PubMed  CAS  Google Scholar 

  19. Voss, B.; Rauterberg, J.; Pott, G., et al. Non-parenchymal cells cultivated from explants of fibrotic liver resemble endothelial and smooth muscle cells from vessel walls. Hepatology 2:19–28; 1982.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil

    Alvaro N. A. Monteiro & Radovan Borojevic

Authors
  1. Alvaro N. A. Monteiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Radovan Borojevic
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Monteiro, A.N.A., Borojevic, R. Complement-dependent induction of DNA synthesis and cell proliferation in human liver connective tissue cells in vitro. In Vitro Cell Dev Biol - Animal 31, 149–155 (1995). https://doi.org/10.1007/BF02633975

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02633975

Key words

Search

Navigation