Summary
The growth and differentiation of an established renal epithelial cell line, LLC-PK1, on membrane bound mussel adhesive protein (MAP), collagen, and extracellular matrix (ECM) in serum-containing medium was studied. Cell attachment and growth on uncoated- vs. protein-coated cellulose nitrate and acetate membranes did not differ significantly, and confluence was achieved on all membranes. However, cells remained in a single monolayer only when plated on collagen or ECM. LLC-PK1 monolayers grown on ECM-coated membranes displayed the highest transepitheliald-glucose transport (333 ± 22 ng·cm−2·min−1) whereas cells plated on collagen-coated membranes displayed the lowest (94 ± 23 ng·cm−2·min−1). Glucose flux values increased with age of the culture, reaching a plateau at 28 d postseeding. These results indicate that the underlying substratum and cell age can affect differentiation of renal epithelial cells in vitro.
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Kinip, T., Galletti, P.M. & Aebischer, P. Effects of attachment substrates on the growth and differentiation of LLC-PK1 cells. In Vitro Cell Dev Biol 26, 162–168 (1990). https://doi.org/10.1007/BF02624108
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DOI: https://doi.org/10.1007/BF02624108