Summary
As part of an effort to optimize conditions required for the complete maturation of muscle cells in vitro, we have investigated the effects of the antibiotics penicillin, streptomycin, and Fungizone (amphotericin B) on the development of cultured chick embryo skeletal muscle. It is shown that even low dosages of streptomycin, but not penicillin or Fungizone, retard protein synthesis and accumulation in these cultures. Myosin accumulation was also reduced and the appearance of striations in fused cells was delayed in myotubes formed in medium containing streptomycin. Additional data suggest that this overall retardation of myogenesis is due to the influence of streptomycin on maturing myotubes rather than early proliferation and cell fusion. These results are discussed with regard to recent efforts to promote the full maturation of muscle cells grown in culture.
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Amonn, F.; Baumann, V.; Weismann, U. N.; Hofman, K.; Herschkowitz, N. Effects of antibiotics on the growth and differentiation in dissociated brain cell cultures. Neuroscience 3: 465–468; 1978.
Bader, D.; Masaki, T.; Fischman, D. A. Immunochemical analysis of myosin heavy chain during avian myogenesis in vivo and in vitro. J. Cell Biol. 95: 763–770; 1982.
Bandman, E.; Matsuda, R.; Strohman, R. C. Developmental appearance of myosin heavy and light chain isoformsin vivo andin vitro in chicken skeletal muscle. Dev. Biol. 93: 508–518; 1982.
Bischoff, R.; Holtzer, H. Mitosis and the process of cell differentiation of myogenic lines in vitro. J. Cell Biol. 41: 188–200; 1969.
Blau, H. M.; Webster, C. Isolation and characterization of human muscle cells. Proc. Natl. Acad. Sci. USA 78: 5623–5627; 1981.
Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254; 1976.
Dolberg, D.; Bissell, M. J. Side effects of amphotericin B-deoxycholate (Fungizone) and nystatin on chick cells in culture. In Vitro 10: 26–29; 1974.
Fischman, D. A. The synthesis and assembly of myofibrils in embryonic muscle. Curr. Top. Dev. Biol. 5: 235–280; 1970.
Hoh, J. F. Y. Developmental changes in chick skeletal myosin isozymes. FEBS Lett. 98: 267–270; 1979.
Koniezcny, S. F.; McKay, J.; Coleman, J. R. Islation and characterization of terminally differentiated chicken and rat skeletal muscle myoblast. Dev. Biol. 91: 11–26; 1982.
Moss, P. S.; Strohman, R. C. Myosin synthesis by fusion-arrested chick embryo myoblasts in cell culture. Dev. Biol. 48: 431–437; 1976.
O’Neill, M.; Stockdale, F. A kinetic analysis of myogenesis in vitro. J. Cell Biol. 52: 52–65; 1972.
O’Neill, M.; Strohman, R. C. Changes in DNA polymerase activity associated with cell fusion in cultures of embryonic muscle. J. Cell Physiol. 73: 61–68; 1969.
Paterson, B.; Strohman, R. C. Myosin synthesis in cultures of differentiating chicken embryo skeletal muscle. Dev. Biol. 29: 128–133; 1972.
Paul, J. Cell and tissue culture. Edinburgh: E. & S. Livingstone; 1970: 135–140.
Perlman, D.; Brindle, S. A. Antibiotic control of contamination in tissue culture. Antimicrob. Agents Chemother. 3: 458–461; 1963.
Shimada, Y. Scanning electron microscopy of myogenesis in monolayer culture: a preliminary study. Dev. Biol. 29: 223–227; 1972.
Walker, C. R.; Bandman, E.; Strohman, R. C. Diazepam induces relaxation of chick embryo muscle fibers in vitro and inhibits myosin synthesis. Exp. Cell Res. 123: 285–291; 1979.
Whalen, R. G.; Sell, S. M.; Bulter-Browne, G. S.; Schwartz, K.; Bouverett, P.; Pinset-Harstrom, I. Three myosin heavy chain isozymes appear sequentially in rat muscle development. Nature 292: 805–809; 1981.
Zak, A. F.; Klimova, N. E.; Iakobson, L. M. Effect of streptomycin preparation on mice, chick embryos and tissue culture. Antibiotiki 12: 480–483; 1967.
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This research was supported by National Institutes of Health Grant NS 155882 and a Task Force on Drug Development Research Contract from The Muscular Dystrophy Association.
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Moss, P.S., Spector, D.H., Glass, C.A. et al. Streptomycin retards the phenotypic maturation of chick myogenic cells. In Vitro 20, 473–478 (1984). https://doi.org/10.1007/BF02619620
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DOI: https://doi.org/10.1007/BF02619620