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Isolation and partial characterization of a human amelogenin from a single fetal dentition using HPLC techniques

  • Molecular and Cellular Biology
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Summary

A strategy based on high pressure liquid chromatography (HPLC) techniques for the isolation or a principal amelogenin molecule from a single human dentition is described. A partial sequence (33 residues) for this 24 kDa amelogenin is presented and related to earlier studies of human 5 kDa tyrosine-rich amelogenin polypeptides (TRAPs). A failure to identify amino acid residue #25 (tryptophan in other amelogenins) suggests that this 24 kDa amelogenin is the progenitor of the human TRAP-2 molecule and provides further support for the possibility of several human amelogenin gene products, generated by splice-junction selection, from the single amelogenin gene in the same individual. Alternatively, multiple amelogenins may arise by expression of both the AMELX and AMELY loci.

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Authors and Affiliations

  1. Department of Basic Sciences (Biochemistry, University of Southern California, Clinical Sciences Annex, 1st Floor 2250 Alcazar Street, Los Angeles, California, USA

    Eduardo Lau

  2. Graduate Program in Craniofacial Biology, University of Southern California, Clinical Sciences Annex, 1st Floor, 2250 Alcazar Street, Los Angeles, California, USA

    Eduardo Lau

  3. Department of Pediatric Pathology, School of Medicine, University of Southern California Women's Hospital, Los Angeles, California, USA

    Zdena Pavlova

  4. Center for Craniofacial Molecular Biology, University of Southern California, Clinical Sciences Annex, 1st Floor 2250 Alcazar Street, 90033, Los Angeles, CA, USA

    Alan G. Fincham, Yiyuan Hu, Harold C. Slavkin & Malcolm L. Snead

Authors
  1. Alan G. Fincham
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  2. Yiyuan Hu
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  3. Eduardo Lau
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  4. Zdena Pavlova
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  5. Harold C. Slavkin
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  6. Malcolm L. Snead
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Fincham, A.G., Hu, Y., Lau, E. et al. Isolation and partial characterization of a human amelogenin from a single fetal dentition using HPLC techniques. Calcif Tissue Int 47, 105–111 (1990). https://doi.org/10.1007/BF02555993

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  • DOI: https://doi.org/10.1007/BF02555993

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