Summary
It has been established that very short patch (VSP) mismatch repair, depending inEscherichia coli on MutL, MutS and Dcm functions, is responsible for the hyper-recombinogenic effect of a class of genetic markers. We show that VSP repair requires the presence of the complete DNA polymerase I enzyme. The absence of endonuclease activities involved in the repair of base-loss sites, Nth, Nfo and Xth, does not affect VSP repair. Implications for the mechanism of the VSP repair are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bailly V, Verly WG (1987)Escherichia coli endonuclease III is not an endonuclease but a β-elimination catalyst. Biochem J 242:565–572
Claverys JP, Lacks SA (1986) Heteroduplex deoxyribonucleic acid base mismatch repair in bacteria. Microbiol Rev 50:133–165
Coulondre C, Miller JH, Farabaugh PJ, Gilbert W (1978) Molecular basis of base substitution hot spots inEscherichia coli. Nature 274:775–780
Cunningham RP, Weiss B (1985) Endonuclease III (nth) mutants ofEscherichia coli. Proc Natl Acad Sci USA 82:474–478
Cunningham RP, Saporito SM, Spitzer SG, Weiss B (1986) Endonuclease IV (nfo) mutant ofEscherichia coli. J Bacteriol 168:1120–1127
Friedberg EC (1985) DNA repair. W.H. Freeman, New York
Jones M, Wagner R, Radman M (1987a) Mismatch repair of deaminated 5-methyl-cytosine. J Mol Biol 193:155–159
Jones M, Wagner R, Radman M (1987b) Mismatch repair and recombination. Cell 50:621–626
Joyce CM, Grindley NDF (1984) Method for determining whether a gene of Escherichia coli is essential: application to thepolA gene. J Bacteriol 158:636–643
Kataoka H, Sekiguchi M (1982) Are purine bases enzymatically inserted into depurinated DNA inEscherichia coli. J Biochem 92:971–973
Lieb M (1983) Specific mismatch correction in bacteriophage lambda crosses by very short patch repair. Mol Gen Genet 191:118–125
Lieb M (1985) Recombination in the lambda repressor gene: very short patch (VSP) mismatch repair restores a specific sequence. Mol Gen Genet 199:465–470
Lieb M (1987) Bacterial genesmutL, mutS anddcm participate in repair of mismatches at 5-methyl-cytosine sites. J Bacteriol 169:118–125
Lieb ME, Allen ED, Read D (1986) Very short patch mismatch repair in phage lambda: repair sites and length of repair tracts. Genetics 114:1041–1060
Lindahl T (1982) DNA repair enzymes. Annu Rev Biochem 56:61–87
Meselson M (1988) Methyl-directed repair of DNA mismatches. In: Low KB (ed) Recombination of genetic material. Academic Press, New York, pp 91–110
Modrich P (1987) DNA mismatch correction. Annu Rev Biochem 56:435–466
Mosbaugh DW, Linn S (1982) Characterization of the action ofEscherichia coli DNA polymerase I at incisions produced by repair endonucleases. J Biol Chem 257:575–583
Radman M, Wagner R (1986) Mismatch repair inEscherichia coli. Annu Rev Genet 20:523–538
Sicard AM, Lefevre JC, Mostachfi P, Gasc AM, Sarda C (1985) Localised conversion inStreptococcus pneumoniae recombination: heteroduplex preference. Genetics 110:557–568
Zell R, Fritz HJ (1987) DNA mismatch-repair inEscherichia coli counteracting the hydrolytic deamination of 5-methyl-cytosine residues. EMBO J 6:1809–1815
Author information
Authors and Affiliations
Additional information
Communicated by R. Devoret
Rights and permissions
About this article
Cite this article
Dzidic, S., Radman, M. Genetic requirements for hyper-recombination by very short patch mismatch repair: Involvement ofEscherichia coli DNA polymerase I. Molec. Gen. Genet. 217, 254–256 (1989). https://doi.org/10.1007/BF02464889
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02464889