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Human gastrin-releasing peptide gene is located on chromosome 18

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Somatic Cell and Molecular Genetics

Abstract

Gastrin-releasing peptide (GRP), a bombesin-like peptide, increases plasma levels of gastrin, pancreatic polypeptide, glucagon, gastric inhibitory peptide, and insulin. GRP is produced in large quantities by small-cell lung cancer and acts as a growth factor for these cells. To determine if chromosomal changes in small-cell lung cancer are related to the expression of GRP, we chromosomally mapped the gene using human-mouse somatic cell hybrids. Twenty hybrids, characterized for human chromosomes, were analyzed by Southern filter hybridization of DNA digested with EcoRI. Human DNA cut with EcoRI yields a major band of 6.8 kb and a minor band of 11.3 kb. The 6.8 kb band segregated concordantly with chromosome 18 and the marker peptidase A. The chromosome 3 abnormalities seen in small-cell lung cancer do not correlate with the chromosomal location of GRP, suggesting that the elevated expression of this gene may be due to mechanisms other than chromosomal rearrangement.

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Authors and Affiliations

  1. Department of Cellular and Structural Biology, The University of Texas, Health Science Center at San Antonio, 7703 Floyd Curl Drive, 78284, San Antonio, Texas

    S. L. Naylor & A. Y. Sakaguchi

  2. Department of Medicine, Brigham and Women's Hospital, Joslin Diabetes Center, Laboratory of Molecular Genetics, 75 Francis Street, 02115, Boston, Massachusetts

    E. Spindel & W. W. Chin

Authors
  1. S. L. Naylor
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  2. A. Y. Sakaguchi
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  3. E. Spindel
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  4. W. W. Chin
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Naylor, S.L., Sakaguchi, A.Y., Spindel, E. et al. Human gastrin-releasing peptide gene is located on chromosome 18. Somat Cell Mol Genet 13, 87–91 (1987). https://doi.org/10.1007/BF02422303

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  • DOI: https://doi.org/10.1007/BF02422303

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