Summary
Five specific transposon-induced nodulation defective (Nod−) mutants from different fast-growing species ofRhizobium were used as the recipients for the transfer of each of several endogenous Sym(biosis) plasmids or for recombinant plasmids that encode early nodulation and host-specificity functions. The Nod− mutants were derived fromR. trifolii, R. meliloti and from a broad-host-rangeRhizobium strain which is able to nodulate both cowpea (tropical) legumes and the non-legumeParasponia. These mutants had several common features (a), they were Nod− on all their known plant hosts, (b), they could not induce root hair curling (Hac−) and (c), the mutations were all located on the endogenous Sym-plasmid of the respective strain. Transfer to these mutants of Sym plasmids (or recombinant plasmids) encoding heterologous information for clover nodulation (pBR1AN, pRt032, pRt038), for pea nodulation (pJB5JI, pRL1JI::Tn1831), for lucerne nodulation (pRmSL26), or for the nodulation of both tropical legumes and non-legumes (pNM4AN), was able to restore root hair curling capacity and in most cases, nodulation capacity of the original plant host(s). This demonstrated a functional conservation of at least some genes involved in root hair curling. Positive hybridization between Nod DNA sequences fromR. trifolii and from a broad-host-rangeRhizobium strain (ANU240) was obtained to other fast-growingRhizobium strains. These results indicate that at least some of the early nodulation functions are common in a broad spectrum ofRhizobium strains.
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Banfalvi Z, Sakanyan Y, Koncz C, Kiss A, Dusha I, Kondorosi A: Location of nodulation and nitrogen fixation genes on a high molecular weight plasmid ofRhizobium meliloti. Mol Gen Genet 184:318–325, 1981.
Banfalvi Z, Randhawa GS, Kondorosi E, Kiss A, Kondorosi A: Construction and characterization of R-prime plasmids carrying symbiotic genes ofR. meliloti. Mol Gen Genet 189:129–135, 1983.
Brewin NJ, Beringer JE, Buchanan-Wollaston AV, Johnston AWB, Hirsch PR: Transfer of symbiotic genes with bacteriocinogenic plasmids inRhizobium leguminosarum. J Gen Microbiol 116:261–270, 1980.
Buchanan-Wollaston AV, Beringer JE, Brewin NJ, Hirsch PR, Johnston AWB: Isolation of symbiotically defective mutants inRhizobium leguminosarum by insertion of transposon Tn5 into a transmissible plasmid. Mol Gen Genet 178:185–190, 1980.
Ditta G, Stanfield S, Corbin D, Helinski DR: Broad host range DNA cloning system for gram-negative bacteria; construction of a gene bank ofRhizobium meliloti. Proc Natl Acad Sci USA 77:7347–7351, 1980.
Djordjevic MA, Zurkowski W, Rolfe BG: Plasmids and stability of symbiotic properties ofRhizobium trifolii. J Bacteriol 151:560–568, 1983.
Djordjevic MA, Zurkowski W, Shine J, Rolfe BG: Sym plasmid transfer to various symbiotic mutants ofRhizobium trifolii, R. leguminosarum, andR. meliloti. J Bacteriol 156:1035–1045, 1983.
Downie JA, Ma QS, Knight CD, Hombrecher G, Johnston AWB: Cloning of the symbiotic region ofRhizobium leguminosarum: the nodulation genes are between the nitrogenase genes andnif A-like gene. EMBO J 2:947–952, 1983a.
Downie JA, Hombrecher G, Ma QS, Knight CD, Wells B, Johnston AWB: Cloned nodulation genes ofRhizobium leguminosarum determine host-range specificity. Mol Gen Genet 190:359–365, 1983b.
Eckhardt T: A rapid method for the identification of plasmid deoxyribonucleic acid in bacteria. Plasmid 1:584–588, 1978.
Hombrecher G, Brewin NJ, Johnston AWB: Linkage of genes for nitrogenase and nodulation ability on plasmids inRhizobium leguminosarum and R. phaseoli. Mol Gen Genet 182:133–136, 1981.
Hooykaas PJJ, Van Brussel AAN, den Dulk-Ras H, van Slogteren GMS, Schilperoort RA: Sym-plasmid ofRhizobium trifolii expressed in different rhizobial species and inAgrobacterium tumefaciens. Nature 291:351–353, 1981.
Johnston AWB, Benyon JL, Buchanan-Wollaston AV, Setchell SM, Hirsch PR, Beringer JE: High frequency transfer of nodulating ability between strains and species ofRhizobium. Nature 276:634–636, 1978.
Kondorosi A, Kondorosi E, Pankhurst CE, Broughton WJ, Banfalvi Z: Mobilization of aRhizobium meliloti megaplasmid carrying nodulation and nitrogen fixation genes into other rhizobia andAgrobacterium. Mol Gen Genet 188:433–439, 1982.
Kondorosi A, Kondorosi E, Banfalvi Z, Broughton WJ, Pankhurst CE, Randhawa GS, Wong C-H, Schell J: Analysis of symbiotic nitrogen fixation genes carried by theRhizobium meliloti megaplasmid. In: Pühler A (ed) Molecular Genetics of the Bacterial-Plant Interaction. Springer-Verlag, Berlin, 1983, pp 55–63.
Kondorosi E, Banfalvi Z, Salska-Kiss C, Kondorosi A: Analysis of symbiotic nitrogen fixation genes carried byRhizobium meliloti megaplasmid. In: Goldberg RB (ed) Plant Molecular Biology, UCLA Symposia on Molecular and Cellular Biology, New Series V.1 XII. Alan R Liss, New York, 1983, pp 259–275.
Long SR, Buikema WJ, Ausubel FM: Cloning ofRhizobium meliloti nodulation genes by direct complementation of Nod− mutants. Nature 298:485–488, 1982.
Marvel DJ, Kuldau G, Hirsch AM, Park J, Torrey JG, Ausubel FM: Cloning and characterisation of a nodulation locus fromRhizobium parasponium. In: Veeger C, Newton WE (eds) Advances in Nitrogen Fixation Research. Nijhoff/Junk, 1984, p 691.
Morrison NA, Cen YH, Trinick MJ, Shine J, Rolfe BG: Heat curing of a Sym plasmid in a fast-growingRhizobium sp. that is able to nodulate legumes and the non-legumeParasponia sp. J Bacteriol 153:527–531, 1983.
Morrison N, Chen HC, Bassam B, Plazinski J, Ridge R, Rolfe B: Tn5 mutagenesis and symbiotic mutants of a fast-growing cowpeaRhizobium. In: Veeger C, Newton WE (eds) Advances in Nitrogen Fixation Research. Nijhoff/Junk, Pudoc, 1984, p 692.
Pankhurst CE, Broughton WJ, Bachem C, Kondorosi I, Kondorosi A: Identification of Nitrogen Fixation and Nodulation genes on a Large Plasmid from a Broad Host RangeRhizobium sp. In: Pühler A (ed) Molecular Genetics of the Bacterial-Plant Interaction. Springer-Verlag, Berlin, 1983, pp 169–176.
Pühler A, Aguilar MD, Hynes M, Müller P, Klipp W, Priefer U, Simon R, Weber G: Advances in the genetics of free-living and symbiotic nitrogen fixing bacteria. In: Veeger C, Newton WE (eds) Advances in Nitrogen Fixation Research. Nijhoff/Junk, 1984, pp 609–619.
Rolfe BG, Gresshoff PM, Shine J: Rapid screening for symbiotic mutants ofRhizobium and white clover. Plant Sci Lett 19:277–284, 1980.
Rolfe BG, Djordjevic M, Scott KF, Hughes JE, Badenoch-Jones J, Gresshoff PM, Cen Y, Dudman WF, Zurkowski W, Shine J: Analysis of the nodule forming ability of fast-growingRhizobium strains. In: Gibson AH, Newton EW (eds) Current Perspectives in Nitrogen Fixation. Australian Academy of Sciences, Canberra, 1984, pp 142–145.
Ruvkun GB, Ausubel FM: A general method for site-directed mutagenesis in procaryotes. Nature 289:85–88, 1981.
Schofield PR, Djordjevic MA, Rolfe BG, Shine J, Watson JM: A molecular linkage map of nitrogenase and nodulation genes inRhizobium trifolii. Mol Gen Genet 192:459–465, 1983.
Schofield PR, Ridge RW, Rolfe BG, Shine J, Watson JM: Host-specific nodulation is encoded on a 14 kb DNA fragment inRhizobium trifolii. Plant Mol Biol (in press).
Scott KF, Hughes JE, Gresshoff PM, Beringer JE, Rolfe BG, Shine J: Molecular cloning ofRhizobium trifolii genes involved in symbiotic nitrogen fixation. J Mol Appl Genet 1:315–326, 1982.
Simon R, Weber G, Arnold W, Pühler A: Analysis of plasmid borne genes inRhizobium meliloti by Tn5 mutagenesis. In: Clark and Stephens (eds) Proceedings of the 8th North American Rhizobium Conference. Winnipeg, Canada: University of Manitoba Press, 1982, pp 67–89.
Southern EM: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–518, 1975.
Vincent JM: A manual for the practical study of root-nodule bacteria. IBP Handbook No. 15 Blackwell Scientific Publications, Oxford, 1970.
Vincent JM: Factors controlling the legume-Rhizobium symbiosis. In: Newton WE, Orme-Johnson WH (eds) Nitrogen Fixation 2. University Park Press, Baltimore, 1980, pp 103–129.
Watson JM, Schofield PR, Ridge RW, Djordjevic MA, Rolfe BG, Shine J: Molecular cloning and analysis of a region of the Sym plasmid ofRhizobium trifolii encoding clover nodulation functions. In: Goldberg RB (ed) Plant Molecular Biology, UCLA Symposia on Molecular and Cellular Biology, new Series Vol XII. Alan R Liss, New York, 1983, pp 303–318.
Yao PY, Vincent JM: Host specificity in the root hair ‘curling factor’ ofRhizobium spp. Aust J Biol Sci 22:413–423, 1969.
Yao PY, Vincent JM: Factors responsible for the curling and branching of clover root hairs byRhizobium. Plant and Soil 45:1–16, 1976.
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Djordjevic, M.A., Schofield, P.R., Ridge, R.W. et al. Rhizobium nodulation genes involved in root hair curling (Hac) are functionally conserved. Plant Mol Biol 4, 147–160 (1985). https://doi.org/10.1007/BF02418762
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DOI: https://doi.org/10.1007/BF02418762