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Seizures induce tenascin-C mRNA expression in neurons

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Journal of Neurocytology

Summary

Tenascin-C, an extracellular matrix glycoprotein that exhibits both growth-promoting and growth-inhibiting properties, is produced in the CNS mainly by astrocytes. In the present study we show that kainate-induced seizures result in an increased expression of tenascin-C in rat brain. Tenascin-C mRNA was increased mainly in the granule cell layer of the hippocampal complex, but tenascin-C mRNA expression was also observed in the pyriform cortex and amygdalo-cortical nucleus. Double labelling experiments using tenascin-C probes and MAP2 (a neuronal microtubule associated protein) antibodies revealed many neurons in these layers that express tenascin-C mRNA. These results support our previous findings of an increased tenascin-C immunoreactivity associated with the axons of granule cells. Tenascin-C expression is rapidly induced by seizures (6h), preceding any lesion and glial reaction. In this pathological condition tenascin-C appears to be produced by both glia and neurons. The functional repercussions on the scarring and remodelling processes are also discussed.

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Authors and Affiliations

  1. Université René Descartes, Paris V, INSERM U29, 123 Bd Port Royal, 75014, Paris, France

    L. Ferhat, N. Chevassus-Au-Louis, M. Khrestchatisky, Y. Ben-Ari & A. Represa

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  1. L. Ferhat
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  2. N. Chevassus-Au-Louis
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  3. M. Khrestchatisky
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  4. Y. Ben-Ari
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  5. A. Represa
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Ferhat, L., Chevassus-Au-Louis, N., Khrestchatisky, M. et al. Seizures induce tenascin-C mRNA expression in neurons. J Neurocytol 25, 535–546 (1996). https://doi.org/10.1007/BF02284821

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  • DOI: https://doi.org/10.1007/BF02284821

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