Summary
Water was added to CO2 by saturation to increase the solvation power of the mobile phase in supercritical fluid chromatography. The saturation was performed at a temperature above the boiling point of water (100°C) to increase the amount of water which could be loaded homogeneously into the CO2 (2.5–3.0 mol% water as compared to about 0.25 mol% water at 25°C). A linear composition of water was produced by altering the density of the CO2 during saturation. Modifications to the injector and CO2 transfer lines prevented phase separation as a result of the instrumentation used in capillary supercritical fluid chromatography (SFC). After fitting vapor-liquid equilibria data to pressure, density, and temperature conditions, approximately 2.5–3.0 mol% of water was introduced in a linear gradient at 110°C. The effect of water on SFC performance was evaluated with standard steroid compounds. This paper provides further evidence for the need to examine vapor-liquid equilibria data prior to SFC.
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Page, S.H., Malik, A., Sumpter, S.R. et al. Demonstration of a linear composition gradient during water saturation of CO2 in supercritical fluid chromatography. Chromatographia 37, 93–97 (1993). https://doi.org/10.1007/BF02272195
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DOI: https://doi.org/10.1007/BF02272195