Abstract
A Pavlovian conditioning experiment was conducted to determine whether morphine (6 mg/kg, IP) could act as a conditioned stimulus (CS) when paired with an electric shock unconditioned stimulus (US), and later produce a conditioned suppression of drinking (CR) in water deprived rats. Seven groups were tested for conditioning after exposure to one of the following conditioning procedures: (1) morphine paired with shock; (2) morphine alone with no shock; (3) shock but no morphine; (4) no shock and no morphine; (5) morphine paired with vocalizations of shocked rats; (6) saline paired with shock; (7) saline alone with no shock. Groups 1 and 2 tested whether morphine could act as a CS. Groups 3 and 4 tested for sensitization. Group 5 tested whether exposure to the vocalizations of other rats could act as a US when paired with a morphine CS. Groups 6 and 7 tested whether cues associated with the injection procedure could act as a CS. Only subjects in group 1 showed conditioned suppression of drinking, when compared to control groups. Overall, the results indicate that morphine could act as a conditioned stimulus and that several of the more obvious possible sources of artifact did not significantly contribute to the CR that it produced.
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References
Carey RJ (1989) Stimulant drugs as conditioned and unconditioned stimuli in a classical conditioning paradigm. Drug Dev Res 16:305–315
Carey RJ (1991) Pavlovian conditioning between co-administered drugs: Elicitation of an apomorphine-induced antiparkinsonian response by scopolamine. Psychopharmacology 104:463–469
Cook L, Davidson A, Davis DJ, Kelleher RT (1960) Epinephrine, norepinephrine and acetylcholine as conditioned stimuli for avoidance behavior. Science 131:990–991
Cunningham CL, Linakis JG (1980) Paradoxical aversive conditioning with ethanol. Pharmacol Biochem Behav 12:337–341
Davey VA, Biederman GB (1991) Methodological issues in drug-drug conditioning in rats: nonassociative factors in heart rate and avfail. Behav Neurosci 105:850–859
Greeley J, Le DA, Poulos CX, Cappell H (1984) Alcohol is an effective cue in the conditional control of tolerance to alcohol. Psychopharmacology 83:159–162
Lett BT (1983) Pavlovian drug-sickness pairings result in the conditioning of an antisickness response. Behav Neurosci 97:779–784
Lett BT (1986) Conditioned slowing of stomach emptying produced by Pavlovian pairings of a drug CS or place with lithium chloride. Psychopharmacology 90:49–53
Lett BT (1992) Pairings of a drug or place conditioned stimulus with lithium chloride produce conditioned sickness, not antisickness. Behav Neurosci 106:106–111
MacQueen GM, Siegel S (1989) Conditional immunomodulation following training with cyclophasphamide. Behav Neurosci 103:638–647
Martin GM (1982) Examination of factors which might disrupt a learned association between pentobarbital and LiCl. Learn Motiv 13:185–199
Martin GM (1983) Effect of pairings of pentobarbital with lithium chloride on the capacity of pentobarbital to maintain a conditioned aversion. Behav Neural Biol 39:145–148
Martin GM, Gans M, van der Kooy, D (1990) Discriminative properties of morphine that modulate associations between tastes and lithium chloride. J Exp Psychol [Animal Behav Proc] 16:56–68
Miczek KA (1991) Tolerance to the analgesic, but not discriminative stimulus effects of morphine after brief social defeat in rats. Psychopharmacology 104:181–186
Overton DA, Shen CF, Tatham TA (1993) Centrally acting drugs act as conditioned stimuli in a conditioned suppression of drinking task. Psychopharmacology 112:270–276
Rescorla RA (1985) Facilitation and inhibition. In: Miller RR, Spear NE (eds) Information processing in animals: conditioned inhibition. Erlbaum, Hillsdale, NJ, pp 299–326
Revusky S (1984) Associative predispositions. In: Marler P, Terrace HS (eds) The biology of learning. Springer, Berlin Heidelberg New York, pp 447–460
Revusky S (1985) Drug interactions measured through taste aversion procedures with an emphasis on medical implications. Ann NY Acad Sci 443:250–271
Revusky S, Reilly S (1990a) Dose effects of heart rate conditioning when pentobarbital is the CS and amphetamine is the US. Pharmacol Biochem Behav 36:933–936
Revusky S, Reilly S (1990b) When pentobarbital is the conditioned stimulus and amphetamine is the unconditioned stimulus, conditioning depends on the type of conditioned response. Behav Neurosci 104:693–703
Revusky S, Taukulis HK, Parker LA, Coombes S (1979) Chemical aversion therapy: Rat data suggest it may be counter-therapeutic to pair an addictive drug state with sickness. Behav Res Ther 17:177–188
Revusky S, Davey V, Zagorski M (1989) Heart rate conditioning with pentobarbital as a conditioned stimulus and amphetamine as an unconditioned stimulus. Behav Neurosc 103:296–307
Ross RT, Holland, PC (1982) Serial positive patterning: Implications for “occasion setting”. Bull Psychon Soc 19:159–162
Silverman PB (1990a) Classically conditioned ethanol stimulus control of a motor behavior. Alcohol 7:489–492
Silverman PB (1990b) Direct dopamine agonist-like activity conditioned to cocaine. Pharmacol Biochem Behav 37:231–234
Silverman PB, Grabowski J, Lane KE (1992) Rotational behavior as a classically conditioned response to pentobarbital administration. Eur J Pharmacol 212:165–169
Taukulis HK (1982) Attenuation of pentobarbital-elicited hypothermia in rats with a history of pentobarbital-LiCl pairings. Pharmacol Biochem Behav 17:695–697
Taukulis HK (1986a) Conditional hyperthermia in response to atropine associated with a hypothermic drug. Psychopharmacology 90:327–331
Taukulis HK (1986b) Conditional shifts in thermic responses to sequentially paired drugs and the “conditional hyperactivity” hypothesis. Pharmacol Biochem Behav 25:83–87
Taukulis HK, Brake LD (1990) Therapeutic and hypothermic properties of diazepam altered by a diazepam-chlorpromazine association. Pharmacol Biochem Behav 34:1–6
Turner EG, Altshuler HL (1976) Conditioned suppression of an operant response usingd-amphetamine as the conditioned stimulus. Psychopharmacology 50:139–143
Wilken LD, Cunningham CL, Fitzgerald RD (1982) Pavlovian conditioning with ethanol and lithium: effects on heart rate and taste aversion in rats. J Comp Physiol Psychol 96:781–790
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Bormann, N.M., Overton, D.A. Morphine as a conditioned stimulus in a conditioned emotional response paradigm. Psychopharmacology 112, 277–284 (1993). https://doi.org/10.1007/BF02244922
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DOI: https://doi.org/10.1007/BF02244922