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Anti-platelet activating factor (PAF) antibody inhibits CFW mouse preimplantation embryo development

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Abstract

Objective

Our purpose was to investigate the effect of anti-PAF antibodies on CFW mouse embryo development in vitro.

Design

We studied the in vitro development of CFW mouse one-cell-stage embryos cultured in MEM supplemented with anti-PAF, anti-IgG, or MEM alone to the hatched blastocyst stage.

Results

Mouse embryos cultured with anti-PAF (1∶5 dilution; 61%) significantly decreased embryo development compared to controls (MEM alone; 93%), whereas embryos cultured in anti-mouse IgG-supplemented MEM (1∶ 10 dilution; 93%) had no effect.

Conclusions

The results provide additional evidence that PAF is produced and secreted by cleavage-stage embryos and is required during the preimplantation period.

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Authors and Affiliations

  1. Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Medical University of South Carolina, 29425-2233, Charleston, South Carolina

    William E. Roudebush, Subbi Mathur & William J. Butler

Authors
  1. William E. Roudebush
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  2. Subbi Mathur
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  3. William J. Butler
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Roudebush, W.E., Mathur, S. & Butler, W.J. Anti-platelet activating factor (PAF) antibody inhibits CFW mouse preimplantation embryo development. J Assist Reprod Genet 11, 414–418 (1994). https://doi.org/10.1007/BF02211729

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  • DOI: https://doi.org/10.1007/BF02211729

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