Summary
Exchange lifetimes of amide protons intrp-repressor with and without the corepressor,l-tryptophan, were studied by heteronuclear 2D NMR spectroscopy. The amide proton exchange times revealed pronounced differences in the stability of different regions of thetrp-repressor. The dimeric core of the molecule is relatively compact and homogeneous in terms of the measured parameters in both apo- and holorepressors. On the other hand the DNA-binding region appears less stable and more susceptible to the exchange of its backbone protons with the solvent. The NMR findings reported here are consistent with and amplify information on the stability of thetrp-repressor obtained by other methods.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Altman, R.B. and Jardetzky, O. (1989) InMethods in Enzymology, Vol. 177, (Eds., Oppenheimer, N.J. and James, T.L.) Academic Press, New York, p. 218–246.
Arrowsmith, C.H., Carey, J., Treat-Clemons, L. and Jardetzky, O. (1989)Biochemistry,28, 3875–3879.
Arrowsmith, C.H., Pachter, R., Altman, R.B., Iyer, S.B. and Jardetzky, O. (1990a)Biochemistry,29, 6332–6341.
Arrowsmith, C.H., Treat-Clemons, L., Szilágyi, L., Pachter, R. and Jardetzky, O. (1990b) InDie Makromolekulare Chemie (Macromolecular Symposia, No. 34) (Eds., Höcker, H. and Sedláçek, B.) Hüthig & Wepf Verlag, Basel, p. 33–46.
Arrowsmith, C.H., Czaplicki, J., Iyer, S.B. and Jardetzky,O. (1991a)J. Am. Chem. Soc.,113, 4020–4022.
Arrowsmith, C.H., Pachter, R., Altman, R. and Jardetzky, O. (1991b)Eur. J. Biochem.,202, 53–66.
Bax, A., Griffey, R.H. and Hawkins, B.L. (1983)J. Magn. Reson.,55, 301–315.
Graddis, T.J., Klig, L.S., Yanofsky, C. and Oxender, D.O. (1988)Proteins,4, 173–181.
Hyde, E.I., Ramesh, V., Roberts, G.C.K., Arrowsmith, C.H., Treat-Clemons, L., Klaic, B. and Jardetzky, O. (1989)Eur. J. Biochem.,183, 545–553.
Kay, L.E., Torchia, D.A. and Bax, A. (1989)Biochemistry,28, 8972–8979.
Klig, L.S., Carey, J. and Yanofsky, C. (1988)J. Mol. Biol.,202, 769–777.
Lane, A.N. and Jardetzky, O. (1987)Eur. J. Biochem.,164, 389–396.
Lawson, C.L., Zhang, R.-G., Schevitz, R.W., Otwinowski, Z., Joachimiak, A. and Sigler, P. (1988)Proteins,3, 18–31.
Otwinowski, Z., Schevitz, R.W., Zhang, R.-G., Lawson, C.L., Joachimiak, A., Marmorstein, R.Q., Luisi, B.F. and Sigler, P.B. (1988)Nature,335, 321–329.
Pachter, R., Altman, R.B., Czaplicki, J. and Jardetzky, O. (1991)J. Magn. Reson.,92, 468–479.
Paluh, J.L. and Yanofsky, C. (1986)Nucl. Acids Res.,14, 7851–7860.
Schevitz, R., Otwinowski, Z., Joachimiak, A., Lawson, C.L. and Sigler, P.B. (1985)Nature,317, 782–786.
Zhang, R.-G., Joachimiak, A., Lawson, C.L., Schevitz, R.W., Otwinowski, Z. and Sigler, P.B. (1987)Nature,327, 591–597.
Author information
Authors and Affiliations
Additional information
Dedicated to the memory of Professor V.F. Bystrov
Rights and permissions
About this article
Cite this article
Czaplicki, J., Arrowsmith, C. & Jardetzky, O. Segmental differences in the stability of thetrp-repressor peptide backbone. J Biomol NMR 1, 349–361 (1991). https://doi.org/10.1007/BF02192859
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02192859