Abstract
Cultures of five out of six strains ofPseudomonas species grown in shaken flasks at 37 C needed tenfold more iron for longevity than for growth. At 30 C, the iron requirement was present in only two, and at 25 C in only one, of the six strains. The metal acted during early stationary phase and, to function, required protein synthesis. In a variety of microorganisms, qualitative and quantitative trace metal requirements for secondary metabolism and culture longevity are identical; we propose that successful completion of the former is a prerequisite of the latter.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bu'Lock, J. D. 1961. Intermediary metabolism and antibiotic synthesis. - Advan. Appl. Microbiol.3: 293–342.
Campbell, J. J. R., Gronlund, A. F. andMenu, P. L. 1968. Low temperature as a means of indicating metabolic pathways. - J. Bacteriol.95: 718–719.
Elander, R. P., Mabe, J. A., Hamill, R. H. andGorman, M. 1968. Metabolism of tryptophans byPseudomonas aureofaciens. VI. Production of pyrrolnitrin by selectedPseudomonas species. - Appl. Microbiol.16: 753–758.
Garibaldi, J. A. 1967. Media for the enhancement of fluorescent pigment production byPseudomonas species. - J. Bacteriol.94: 1296–1299.
Komai, H. andNeilands, J. B. 1968. Effect of zinc ions on δ-aminolevulinate dehydratase inUstilago sphaerogena. - Arch. Biochem. Biophys.124: 456–461.
Kurachi, M. 1958. Studies on the biosynthesis of pyocyanine. (III) On the effect of iron and other heavy metals. - Bull. Inst. Chem. Research, Kyoto University36: 188–196.
Leonard, C. G., Housewright, R. D. andThorne, C. B. 1958. Effects of some metallic ions on glutamyl polypeptide synthesis byBacillus subtilis. - J. Bacteriol.76: 499–503.
McLean, R. A., Lilly, H. D. andAlford, J. A. 1968. Effects of meat-curing salts and temperature on production of staphylococcal enterotoxin B. - J. Bacteriol.95: 1207–1211.
Schindler, A. F., Palmer, J. G. andEisenberg, W. V. 1967. Aflatoxin production byAspergillus flavus as related to various temperatures. - Appl. Microbiol.15: 1006–1009.
Smith, D. D. andWyss, O. 1969. The rapid loss of viability ofAzotobacter in aqueous solutions. - Antonie van Leeuwenhoek35: 84–96.
Steenbergen, J. F., Steenbergen, S. M. andWeinberg, E. D. 1969. Tolerance of yeasts to zinc: distinction between cell growth and cell longevity. - Can. J. Microbiol.15: 229–233.
Totter, J. R. andMoseley, F. T. 1953. Influence of the concentration of iron on the production of fiuorescin byPseudomonas aeruginosa. - J. Bacteriol.65: 45–47.
Weinberg, E. D. 1969. Biosynthesis of secondary metabolites: roles of trace metals. -Advan. Microb. Physiol.4: 1–44.
Weinberg, E. D. andTonnis, S. M. 1967. Role of manganese in biosynthesis of bacitracin. - Can. J. Microbiol.13: 614–615.
Wensinck, F., van Dalen, A. andWedema, M. 1967. Iridescent material and the effect of iron on its production byPseudomonas aeruginosa. - Antonie van Leeuwenhoek33: 73–86.
Author information
Authors and Affiliations
Additional information
This work was supported in part by grant HD-03038-02 from the National Institute of Child Health and Human Development, U. S. Public Health Service.
We express our appreciation to Miss Patsy Linn Person for performing preliminary experiments. A portion of the material was presented at the Annual Meeting of the American Society for Microbiology in Miami Beach, Florida, 4–9 May, 1969.
Rights and permissions
About this article
Cite this article
Weinberg, E.D., Goodnight, S.A. Iron requirement for longevity ofPseudomonas cultures. Antonie van Leeuwenhoek 36, 73–80 (1970). https://doi.org/10.1007/BF02069010
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02069010