Skip to main content
SpringerLink
Log in

On the mechanism of killing of Trypanosoma cruzi by human polymorphonuclear leukocytes

  • Short Communications
  • Immunology
  • Published:
Experientia Aims and scope Submit manuscript

Summary

The temperature-dependence of some processes involved in the killing of sensitizedT. cruzi epimastigotes by human polymorphonuclear leukocytes (PMN) was determined. The rate of the reactions was related to the temperature of incubation according to the Arrhenius equation and the apparent energies of activation (Ea) were calculated. The Ea values separated these complex reactions into two groups: one with Ea of about 10 kcal/mol for the phagocytosis of the parasites and the release of lysosomal enzymes by PMN, and the other with Ea of about 22 kcal/mol for the cytotoxicity against sensitizedT. cruzi, the rate of oxygen consumption by PMN, and the lysis of the parasites with added hydrogen peroxide.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Literatur

  1. Olabuenaga, S.E., Cardoni, R.L., Segura, E.L., Riera, N.E., and de Bracco, M.M.E., Cell Immun.45 (1979) 85.

    Article  CAS  Google Scholar 

  2. Madeira, E., Andrade, A., Bunn-Moreno, M.M., and Barcinski, M., Infect. Immunity25 (1979) 34.

    Article  CAS  Google Scholar 

  3. Villalta, F., and Kierszenbaum, F., J. Immun.131 (1983) 1504.

    Article  CAS  PubMed  Google Scholar 

  4. Rimoldi, M.T., Cardoni, R.L., Olabuenaga, S.E., and de Bracco, M.M.E., Immunology42 (1981) 521.

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Cardoni, R.L., Docampo, R., and Casellas, A.M., J. Parasit.68 (1982) 547.

    Article  CAS  PubMed  Google Scholar 

  6. Cardoni, R.L., Rimoldi, M.T., Katzin, V.J., Gonzalez Cappa, S.M., and de Bracco, M.M.E., Medicina (Bs. As.)42 (1982) 495.

    CAS  Google Scholar 

  7. de Titto, E.H., and Cardoni, R.L., Exp. Parasit.56 (1983) 247.

    Article  PubMed  Google Scholar 

  8. Docampo, R., Casellas, A.M., Madeira, E.D., Cardoni, R.L., Moreno, S.N.J., and Mason, R.P., FEBS Lett.155 (1983) 25.

    Article  CAS  PubMed  Google Scholar 

  9. Keeling, P.J., and Henson, P., J. Immun.128 (1983) 563.

    Article  Google Scholar 

  10. Klebanoff, S.J., in: Advances in Host Defense Mechanisms, p. 111. Raven Press, New York 1982.

    Google Scholar 

  11. Sanderson, C.J., Bunn-Moreno, M.M., and Lopez, A.F., Parasitology76 (1978) 299.

    Article  CAS  PubMed  Google Scholar 

  12. Fishman, W.H., Kato, K., Anstiss, C.L., and Green, S., Clinica chim. Acta15 (1967) 435.

    Article  CAS  Google Scholar 

  13. Litwack, G. Proc. Soc. exp. Biol. Med.84 (1955) 401.

    Article  Google Scholar 

  14. Bull, H.B., in: An Introduction to Physical Biochemistry, p. 390. F.A. Davis Co., Philadelphia 1971.

    Google Scholar 

  15. Nathan, C.F., Nogueira, N., Juangbhanich, C., Ellis, J., and Cohn, Z.A., J. exp. Med.149 (1979) 1056.

    Article  CAS  PubMed  Google Scholar 

  16. Mahoney, E.M., Halmill, A.L., Scott, W.A., and Cohn, Z.A., Proc. natl. Acad. Sci. USA74 (1977) 4895.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Badwey, J.A., Curnutte, J.T., Robinson, J.M., Lazdings, J.K., Briggs, R.T., Karnovsky, M.J., and Karnovsky, M.L., J. Cell Physiol.105 (1980) 541.

    Article  CAS  PubMed  Google Scholar 

  18. Boveris, A., and Stoppani, A.O.M. Experientia33 (1977) 1306.

    Article  CAS  PubMed  Google Scholar 

  19. Fridovich, I., A. Rev. Biochem.44 (1975) 147.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Instituto Nacional de Diagnóstico e Investigación de la Enfermedad de Chagas ‘Dr. M. Fatala Chabén’, Av. Paseo Colón 568, 1063, Buenos Aires, Argentina

    R. L. Cardoni, E. H. de Titto & R. Docampo

  2. Department of Health and Human Service, National Institute of Environmental Health Science, 27709, Research Triangle Park, North Carolina, USA

    R. L. Cardoni, E. H. de Titto & R. Docampo

Authors
  1. R. L. Cardoni
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. H. de Titto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Docampo
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported by research grants from CONICET and SUBCYT, Argentina, and UNDP/Word Bank/WHO Special Programme for Research and Training in Tropical Diseases. The author wish to thank Dr I. Reisin, Dr. A. Boveris and Dr M.M.E. de Bracco for their helpful discussion.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cardoni, R.L., de Titto, E.H. & Docampo, R. On the mechanism of killing of Trypanosoma cruzi by human polymorphonuclear leukocytes. Experientia 41, 89–91 (1985). https://doi.org/10.1007/BF02005891

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02005891

Key words

Search

Navigation