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Effect of volatile anesthetics on the circular dichroism of bilirubin bound to human serum albumin

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Abstract

The characteristic circular dichroism of bilirubin bound to human serum albumin undergoes a remarkable sign inversion on addition of halothane, chloroform and other volatile anesthetics. This sign inversion, which is completely reversed by removal of the anesthetic, reflects a pronounced conformational change of the bound ligand; probably a complete inversion of chirality. The observation suggests that association of volatile anesthetics with proteins can markedly alter the internal topography of receptor sites and potentially influence the stereoselectivity of ligand binding.

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Authors and Affiliations

  1. The Liver Center, Gastrontestinal Research Unit, University of California San Francisco, 94143-0538, San Francisco, California, USA

    A. F. McDonagh

  2. Cell and Molecular Biology Program, Department of Chemistry, University of Nevada, 89557-0020, Reno, Nevada, USA

    Y. -M. Pu & D. A. Lightner

Authors
  1. A. F. McDonagh
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  2. Y. -M. Pu
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  3. D. A. Lightner
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McDonagh, A.F., Pu, Y.M. & Lightner, D.A. Effect of volatile anesthetics on the circular dichroism of bilirubin bound to human serum albumin. Experientia 48, 246–248 (1992). https://doi.org/10.1007/BF01930465

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  • DOI: https://doi.org/10.1007/BF01930465

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