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Transtubular bioreactor: A perfusion device for mammalian cell cultivation

  • Boreactor and Reactor Performance
  • Published:
Biotechnology Techniques

Summary

A transtubular mammalian cell perfusion chamber has been developed that allows separate delivery and control of medium and gas flow to the cells. The hydrodynamic and mass transfer characteristics of the reactor are studied experimentally for both gas and liquid components. Feasibility and potential of the transtubular reactor are demonstrated by the growth of a hybridoma cell line.

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References

  • Boraston, R., Thompson, P. W., Garland, S. and Birch, J. R.,Develop. Biol. Standard.,55, 103–111, 1982.

    Google Scholar 

  • Claflin, J. L. and Davie, J. M.,J. Immunol.,114, 70–75, 1975.

    Google Scholar 

  • Glacken, M. W., Fleischaker, R. J. and Sinskey, A. J..Ann. New York Academy of Sciences,413, 3 55–372, 1983.

    Google Scholar 

  • Halberstadt, C. R. and Midgley, A. R., Patent pending, Bioreactor System, April, 18, 1988.

  • Hu, W-S and Sinskey, A. J.Genetic Engineering News,8 (5), 6, May, 1988.

    Google Scholar 

  • Hu, W-S and Wang, D.I.C. inMammalian Cell Technology, W. G. Thilly, ed., Butterworths, Boston 167–197, 1986.

    Google Scholar 

  • Lavery, M., and Nienow, A. W.,Biotech. Bioeng.,30, 369–373 (1987).

    Google Scholar 

  • Levenspiel, O.,Chemical Reaction Engineering, 2nd ed., John Wiley, New York, 1972.

    Google Scholar 

  • Thilly, W. G., ed.,Mammalian Cell Technology, Butterworths, Boston, 1986.

    Google Scholar 

  • van Wezel, A. L., van der velden-de Groot, C. A. M., de Haan, H. H., van den Heuvel N. and Scharfoort, R., “Large Scale Animal Cell Cultivation for Production of Cellular Biochemicals”.Develop. Biol. Stand.,60, 229–236, 1985.

    Google Scholar 

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Authors and Affiliations

  1. Department of Chemical Engineering, The University of Michigan, 48109, Ann Arbor, MI

    Sadettin S. Ozturk & Bernhard O. Palsson

  2. Consortium for Research in Developmental and Reproductive Biology, The University of Michigan, 48109, Ann Arbor, MI

    A. Rees Midgley & Craig R. Halberstadt

  3. BioQuant, Inc., 48109, Ann Arbor, MI

    A. Rees Midgley

Authors
  1. Sadettin S. Ozturk
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  2. Bernhard O. Palsson
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  3. A. Rees Midgley
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  4. Craig R. Halberstadt
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Ozturk, S.S., Palsson, B.O., Midgley, A.R. et al. Transtubular bioreactor: A perfusion device for mammalian cell cultivation. Biotechnol Tech 3, 55–60 (1989). https://doi.org/10.1007/BF01876222

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  • DOI: https://doi.org/10.1007/BF01876222

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