Summary
Cellular potential and pH measurements (pH i ) were carried out in the perfused kidney ofNecturus on proximal tubules with standard and recessed-tip glass microelectrodes under control conditions and after stimulation of tubular bicarbonate reabsorption. Luminal pH and net bicarbonate reabsorption were measured in parallel experiments with recessed-tip glass or antimony electrodes, both during stationary microperfusions as well as under conditions of isosmotic fluid transport. A mean cell pH of 7.15 was obtained in control conditions. When the luminal bicarbonate concentration was raised to 25 and 50mm, pH i rose to 7.44 and 7.56, respectively. These changes in pH i were fully reversible. Under all conditions intracellular H+ was below electrochemical equilibrium. Thus the maintenance of intracellular pH requires “active” H+ extrusion across one or both of the cell membranes. The observed rise in pH i and the peritubular depolarization after stimulation of bicarbonate reabsorption are consistent with enhanced luminal hydrogen ion secretion and augmentation of peritubular bicarbonate exit via an anion-conductive transport pathway.
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O'Regan, M.G., Malnic, G. & Giebisch, G. Cell pH and luminal acidification inNecturus proximal tubule. J. Membrain Biol. 69, 99–106 (1982). https://doi.org/10.1007/BF01872269
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DOI: https://doi.org/10.1007/BF01872269