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Functional reconstitution of lens gap junction proteins into proteoliposomees

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Summary

Membranes rich in junction complexes were prepared from bovine lens, and the fragments of the membranes were reconstituted into proteoliposomes with a large excess of phosphatidylcholine and dicetylphosphate. The osmotic swelling behavior of these liposomes showed that the lens junction membranes contributed protein components that produced channels with a nominal diameter of 1.4 nm. Most preparations of lens junctions produced rates of osmotic swelling much slower than those found in proteoliposomes containing equivalent amounts ofEscherichia coli porin, and we discuss several possible explanations for this observation.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, University of California, 94720, Berkeley, California

    Hiroshi Nikaido & Emiko Y. Rosenberg

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  1. Hiroshi Nikaido
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  2. Emiko Y. Rosenberg
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Nikaido, H., Rosenberg, E.Y. Functional reconstitution of lens gap junction proteins into proteoliposomees. J. Membrain Biol. 85, 87–92 (1985). https://doi.org/10.1007/BF01872008

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  • DOI: https://doi.org/10.1007/BF01872008

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