Summary
The patch-clamp technique was used to characterize ion channels in the apical membranes of cultured human nasal epithelial cells, dissociated from fetal nasal mucosa and from adult nasal polyps. Outward-rectifying chloride channels were found in 4.3% of the cell-attached patches from fetal cells (n=258) and in 3.1% of the patches from adult cells (n=320). After exeision the number of patches containing active chloride channels increased threefold to 13% of the patches from the fetal cells and 10% from adult cells. The single-channel conductance at 0 mV in symmetrical 150mm NaCl solutions was 24.3 ±0.9 pS (n=28) and 26.0 ± 1.2 pS (n=30), respectively, in adult and fetal cells and showed outward rectification in the potential range from −80 to +80 mV. In fetal cells as well as in adult cells the channels were anion selective, and were almost impermeable for larger anions and monovalent cations. In cell-free patches the channels were Ca2+ independent. In most of the channels the open probability was voltage independent and high (±0.86); in 20% of the channels, however, the open probability increased with depolarization. In conclusion, fetal nasal epithelial cells contain chloride channels in their apical membranes with singlechannel properties and regulatory mechanisms similar to those found in cells from adults.
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References
Al Bazzaz, F.J. 1981. Role of cyclic AMP in regulation of chloride secretion by canine tracheal mucosa.Am. Rev. Respir. Dis. 123:295–298
Bear, C.E. 1988. Phosphorylation-activated chloride channels in human skin fibroblasts.FEBS Lett. 237:145–149
Chen, J.H., Schulman, H., Gardner, P. 1989. A cAMP-regulated chloride channel in lymphocytes that is affected in cystic fibrosis.Science 243:657–660
Cotton, C.U., Stutts, M.J., Knowles, M.R., Gatzy, J.T., Boucher, R.C. 1987. Abnormal apical cell membrane in cystic fibrosis respiratory epithelium. An in vitro electrophysiological analysis.J. Clin. Invest. 79:80–85
Dreinhofer, J., Gögelein, H., Greger, R. 1988. Blocking kinetics of Cl-channels in colonic carcinoma cells (HT29) as revealed by 5-nitro-2-(3-phenylpropylamino(benzoic acid (NPPB).Biochim. Biophys. Acta 946:135–142
Finkelstein, R.A.,1973. Cholera.Crit. Rev. Microbiol. 2:552–623
Frizzell, R.A., Halm, D.R., Rechkemmer, G., Shoemaker, R.L. 1986a. Chloride channel regulation in secretory epithelia.Fed. Proc. 45:2727–2731
Frizzell, R.A., Rechkemmer, G., Shoemaker, R.L. 1986b. Altered regulation of airway epithelial cell chloride channels in cystic fibrosis.Science 233:558–560
Gill, D.M., King, C.A. 1975. The mechanism of action of cholera toxin in pigeon erythrocyte lysates.J. Biol. Chem. 250:6424–6432
Gögelein, H. 1988. Chloride channels in epithelia.Biochim. Biophys. Acta 947:521–547
Greger, R., Schlatter, E., Gögelein, H. 1987. Chloride channels in the luminal membrane of the rectal gland of the dogfish (Squalus acanthias).Pfluegers Arch. 409:114–121
Gruenert, D.C., Basbaum, C.B., Welsh, M.J., Li, M., Finkbeiner, W.E., Nadel, J.A. 1988. Characterization of human tracheal epithelial cells transformed by an origin-defective simian virus 40.Proc. Natl. Acad. Sci. USA 85:5951–5955
Halm, D., Schoumacher, R., Frizzell, R. 1987. Biophysical properties of an apical membrane chloride channel in a secretory epithelial cell line. T84.Fed. Proc. 46:636abstr. no: 1863)
Hamill, O.P., Marty, A., Neher, E., Sakmann, B., Sigworth, F.J. 1981. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.Pfluegers Arch. 391:85–100
Hayslett, J.P., Gögelein, H., Kunzelmann, K., Greger, R. 1987. Characteristics of apical chloride channels in human colon cells (HT29).Pfluegers Arch. 410:487–494
Jorissen, M., Van der Schueren, B., Van den Berghe, H., Cassiman, J.J. 1989. Preservation and regeneration of cilia on human nasal epithelial cells cultured in vitro.Arch. Otorhinolaryngol. 246:308–314
Jorissen, M., Vereecke, J., Carmeliet, E., Cassiman, J.J. 1990. Identification of a voltage-and calcium-dependent nonselective cation channel in cultured adult and fetal human nasal epithelial cells.Pfluegers Arch. 415:617–623
Krouse, M.E., Hagiwara, G., Lewiston, N.J., Wine, J.J. 1987. Ion channel activity in apical membranes of cultured sweat gland secretory cells.Ped. Pulmonol. 114(Suppl. 1)
Li, M., McCann, J.D., Liedtke, C.M., Nairn, A.C., Greengard, P., Welsh, M.J. 1988. Cyclic AMP-dependent protein kinase opens chloride channels in normal but not cystic fibrosis airway epithelium.Nature (London) 331:358–360
Poulsen, J., Tos, M. 1975. Goblet cells in the developing human nose.Acta Otolaryngol. 80:434–442
Reinhardt, R., Bridges, R.J., Rummel, W., Lindemann, B. 1987. Properties of an anion-selective channel from rat colonic enterocyte plasma membranes reconstitued into planar phospholipid bilayers.J.Membrane Biol. 95:47–54
Schoumacher, R.A., Shoemaker, R.L., Frizzell, R.A. 1987a. Abnormal regulation of apical membrane chloride channels in sweat gland secretory cells in cystic fibrosis (CF).Fed. Proc. 46:1272 (iitabstr. no. 5570)
Schoumacher, R.A., Shoemaker, R.L., Halm, D.R., Tallant, E.A., Wallace, R.W., Frizzell, R.A. 1987b. Phosphorylation fails to activate chloride channels from cystic airway cells.Nature (London) 330:752–754
Shoemaker, R.L., Frizzell, R.A., Dwyer, T.M., Farley, J.M. 1986. Single chloride channel currents from canine tracheal epithelial cells.Biochim. Biophys. Acta 858:235–242
Smith, P.L., Welsh, M.J., Stoff, J.S., Frizzell, R.A. 1982. Chloride secretion by canine tracheal epithelium. I. Role of intracellular cAMP levels.J. Membrane Biol. 70:217–226
Welsh, M.J. 1985. Ion transport by primary cultures of canine tracheal epithelium: Methodology, morphology, and electrophysiology.J. Membrane Biol. 88:149–163
Welsh, M.J. 1986a. An apical-membrane chloride channel in human tracheal epithelium.Science 232:1648–1650
Welsh, M.J. 1986b. Single apicalmembrane anion channels in primary cultures of canine tracheal epithelium.Pfluegers Arch. 407(Suppl. 2)S116-S122
Welsh, M.J. 1987. Electrolyte transport by airway epithelia.Physiol. Rev. 67:1143–1184
Welsh, M.J., Liedtke, C.M. 1986. Chloride and potassium channels in cystic fibrosis airway epithelia.Nature (London) 322:467–470
Welsh, M.J., LcCann J.D., Dearborn, D.G. 1987. Chloride and sodium channel currents in normal and cystic fibrosis sweat gland epithelium.Fed. Proc. 46:1271 (abstr. no. 5567)
Widdicombe, J.H., Welsh, M.J., Finkbeiner, W.E. 1985. Cystic fibrosis decreases the apical membrane chloride permeability of monolayers cultured from cells of tracheal epithelium.Proc. Natl. Acad. Sci. USA 82:6167–6171
Yankaskas, J.R., Cotton, C.U., Knowles, M.R., Gatzy, J.T., Boucher, R.C. 1985a. Culture of human nasal epithelial cells on collagen matrix supports. A comparison of bioelectric properties of normal and cystic fibrosis epithelia.Am. Rev. Respir. Dis. 132:1281–1287
Yankaskas, J.R., Knowles, M.R., Gatzy, J.T., Boucher, R.C. 1985b. Persistence of abnormal chloride ion permeability in cystic fibrosis nasal epithelial cells in heterologous culture.Lancet 1:954–956
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Jorissen, M., Vereecke, J., Carmeliet, E. et al. Outward-rectifying chloride channels in cultured adult and fetal human nasal epithelial cells. J. Membrain Biol. 117, 123–130 (1990). https://doi.org/10.1007/BF01868679
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DOI: https://doi.org/10.1007/BF01868679