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Multiple conductance levels of the dihydropyridine-sensitive calcium channel in GH3 cells

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Summary

Calcium channels in GH3 cells exhibit at least five conductance levels when examined in cell-attached or outside-out patches. These channels resemble the high threshold Ca2+ current in their range of activation and inactivation, and in their sensitivity to dihydropyridines (DHP). Mean open times for the five levels were brief (<1 msec) in control solutions but increased in the presence of BAY K 8644. In 100mm Ba2+ and BAY K 8644, the five predominant slope conductances were 8–9, 12–13, 16–18, 23–24, and 28 pS. The present study is the first report of multiple levels of the DHP-sensitive Ca2+ channel occurring with high frequency in native membranes. The range of conductance levels that we observed encompasses the range of conductances found for two other different types of Ca2+ channels and indicates that unit conductance should be used with caution as a distinguishing characteristic for identification of different channel types.

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Authors and Affiliations

  1. Department of Physiology and Molecular Biophysics, Baylor College of Medicine, 77030, Houston, Texas

    Diana L. Kunze

  2. Department of Physiology and Biophysics, University of Texas Medical Branch, 77550, Galveston, Texas

    Aileen K. Ritchie

Authors
  1. Diana L. Kunze
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  2. Aileen K. Ritchie
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Kunze, D.L., Ritchie, A.K. Multiple conductance levels of the dihydropyridine-sensitive calcium channel in GH3 cells. J. Membrain Biol. 118, 171–178 (1990). https://doi.org/10.1007/BF01868474

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  • DOI: https://doi.org/10.1007/BF01868474

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