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Internal and external application of photodynamic sensitizers on squid giant axons

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Summary

Squid giant axons were photosensitized by dyes applied internally or externally in air saturated solutions and photochemically modified by visible light. For most dyes the modifications included an irreversible block of sodium channels, a destruction of inactivation in some of the unblocked channels, and a slowing of inactivation. Internal application was up to 100-fold more effective in blocking sodium channels than external application, suggesting a site of block nearer the internal surface. Rose Bengal sensitized channel block and destruction of inactivation when applied internally, but sensitized only channel block when applied externally. In contrast, externally applied Eosin Y sensitized a clear slowing of inactivation plus channel block. Beta-carotene, an effective agent for quenching photochemically generated excited singlet oxygen, inhibited most of the modification sensitized by internally applied Methylene blue but not by Rose Bengal or Merocyanine 540.

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Author notes

  1. G. S. Oxford

    Present address: Department of Physiology, University of North Carolina at Chapel Hill, Medical Sciences Research Wing 206H, 27514, Chapel Hill, N.C.

Authors and Affiliations

  1. Department of Physiology, Emory University, 30322, Atlanta, Ga.

    G. S. Oxford, J. P. Pooler & T. Narahashi

  2. Department of Physiology and Pharmacology, Duke University Medical Center, 27710, Durham, North Carolina

    G. S. Oxford, J. P. Pooler & T. Narahashi

Authors
  1. G. S. Oxford
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  2. J. P. Pooler
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  3. T. Narahashi
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Oxford, G.S., Pooler, J.P. & Narahashi, T. Internal and external application of photodynamic sensitizers on squid giant axons. J. Membrain Biol. 36, 159–173 (1977). https://doi.org/10.1007/BF01868149

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  • DOI: https://doi.org/10.1007/BF01868149

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