Skip to main content
SpringerLink
Log in

The use of ribonuclease U2 in RNA sequence determination

Some Corrections in the Catalog of Oligomers Produced by Ribonuclease T, Digestion ofEscherichia coli 16S Ribosomal RNA

  • Published:
Journal of Molecular Evolution Aims and scope Submit manuscript

Abstract

The catalog of oligomers produced by ribonuclease T1 digestion ofEscherichi coli 16S ribosomal RNA has been determined by a new method that involves the use of ribonuclease U2 fromUstilago sphaerogena. The sequences for the larger T1 oligomers (8 or more bases) determined in this way differ in more than 50 % of the cases from those reported previously (determined by other methods).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Arima, T., Uchida, T., Egami, F.: Biochem. J.106, 601 (1968)

    PubMed  Google Scholar 

  • Doolittle, W. F., Pace, N. R.: Proc. Nat. Acad. Sci. (Wash.)68, 1786 (1971)

    Google Scholar 

  • Fellner, P., Ehresmann, C., Ebel, J. P.: Biochimie54, 880 (1972a)

    Google Scholar 

  • Fellner, P., Ehresmann, C., Stiegler, P., Ebel, J. P.: Nature, New Biol. (Lond.)239, 1 (1972b)

    Google Scholar 

  • Fellner, P., Sanger, F.: Nature (Lond.)219, 236 (1968)

    Google Scholar 

  • Franklin, R. M.: Proc. Nat. Acad. Sci. (Wash.)55, 1504 (1966)

    Google Scholar 

  • Kirby, K. S.: Biochem. J.64, 405 (1956)

    PubMed  Google Scholar 

  • Pechman, K. J., Woese, C.: J. Molec. Evolution1, 230 (1972)

    Google Scholar 

  • Sanger, F., Brownlee, G. G., Barrell, B. G.: J. Molec. Biol.13, 373 (1965)

    PubMed  Google Scholar 

  • Sogin, M., Pace, B., Pace, N. R., Woese, C.: Nature (Lond.)232, 48 (1971)

    Google Scholar 

  • Sogin, M., Woese, C., Pace, B., Pace, N. R.: J. Molec. Evolution2, 167 (1973)

    Google Scholar 

  • Uchida, T., Arima, T., Egami, F.: J. Biochem. (Tokyo)67, 91 (1970)

    Google Scholar 

Download references

Author information

Author notes

  1. T. Uchida

    Present address: Mitsubishi-Kasei Institute of Life Sciences, Machida, Tokyo, Japan

Authors and Affiliations

  1. Department of Microbiology, University of Illinois, 61801, Urbana, Illinois, USA

    T. Uchida, L. Bonen, H. W. Schaup, B. J. Lewis, L. Zablen & C. Woese

Authors
  1. T. Uchida
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. Bonen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. W. Schaup
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. B. J. Lewis
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. L. Zablen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. C. Woese
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Uchida, T., Bonen, L., Schaup, H.W. et al. The use of ribonuclease U2 in RNA sequence determination. J Mol Evol 3, 63–77 (1974). https://doi.org/10.1007/BF01795977

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01795977

Key words

Search

Navigation