Abstract
In an effort to generate an A.CA mouse expressing Ed, the Ead gene has been introduced into A. CA mice which lack the major histocompatibility complex (MHC) class II E molecule. Flow cytometric analysis shows cell surface expression of the Eα chain on lymphocytes and macrophages in the transgenic mice. Analysis of T-cell receptor (Tcr) genes deleted in some E-expressing mouse strains demonstrates that T cells expressingTcrb-VS are partially deleted in these transgenic mice while those expressingTcrb-V8 andTcrb-11 are not. In addition, the expressed Eαd chain can promote Mycoplasma arthriditis mitogen (MAM)-induced T-cell proliferation. The expression of the Ea chain, presumably as an Aß fEαd heterodimer, can alter the peripheral T-cell repertoire and T-cell reactivity to a microbial superantigen.
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Ishikawa, S., Chang, Md.Y. & Diamond, B. Alteration of the T-cell receptor repertoire in A. CA mice expressing anEa d transgene. Immunogenetics 33, 178–183 (1991). https://doi.org/10.1007/BF01719237
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DOI: https://doi.org/10.1007/BF01719237