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Micronucleation of human somatic cells with colcemid

  • Published:
Journal of tissue culture methods

Summary

A method is described for the production with colcemid of micronucleate cells from normal human fibroblasts (Detroit 550) and human aneuploid D98/AH2 cells. The colcemid dose is titrated to a concentration that prevents cellular attachment to, and spreading on, the substrate following attempted mitosis. Washing out the drug with growth medium followed by incubation in drug-free medium for 12 to 24 h produces micronucleate cells. The percentage of micronucleation obtainable is 50 to 70% by treatment with 0.1 ug/ml colcemid for 24 h followed by incubation of floating blocked mitotic cells in drug-free growth medium.

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References

  1. Margulis, L. Colchicine-sensitive microtubules. Int. Rev. Cytol. 34: 333–361; 1973.

    Google Scholar 

  2. Shay, J. W.; Clark, M. A. Morphological studies on the enucleation of colchicine treated L-929 cells. J. Ultrastruct. Res. 58: 155–159; 1977.

    Google Scholar 

  3. Ege, T.; Krondahl, U.; Ringertz, N. R. Introduction of nuclei and micronuclei into cells and enucleated cytoplasms by Sendai virus induced fusion. Expt. Cell Res. 88: 428–432; 1974.

    Google Scholar 

  4. Ege, T.; Ringertz, N. R. Preparation of micro-cells by enucleation of micronucleate cells. Expt. Cell Res. 88: 378–382; 1974.

    Google Scholar 

  5. Fournier, R. E. K.; Ruddle, F. H. Microcell-mediated transfer of murine chromosomes into mouse, Chinese hamster, and human somatic cells. Proc. Natl. Acad. Sci. USA. Sci. USA 74: 319–323; 1977.

    Google Scholar 

  6. Johnson, R. T.; Mullinger, A. M.; Downes, C. S. Human minisegregant cells. In: Prescott, D. M. ed., Methods in cell biology. vol 20. Academic Press: New York; 255–314; 1978.

    Google Scholar 

  7. Crenshaw, A. H.; Shay, J. W.; Murrell, L. R. Colcemid-induced micronucleation in cultured human cells. J. Ultrastruct. Res. 75: 179–186; 1981.

    Google Scholar 

  8. Cremer, T.; Zorn, C.; Cremer, C.; Zimmer, J. Formation of viable cell fragments by treatment with colchicine. Exp. Cell Res. 100: 345–355; 1976.

    Google Scholar 

  9. Cheung, H. T.; Cantarow, W. D.; Sudharadas, G. Colchicine and cytochalasin B effects on random movement, spreading and adhesion of mouse macrophages. Expt. Cell Res. 111: 95–103; 1978.

    Google Scholar 

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Authors and Affiliations

  1. Department of Anatomy, University of Tennessee Center for the Health Sciences, 38163, Memphis, Tennessee, USA

    Andrew H. Crenshaw Jr. & Leonard R. Murrell

  2. Department of Cell Biology, University of Texas Health Science Center, 75235, Dallas, Texas, USA

    Jerry W. Shay

Authors
  1. Andrew H. Crenshaw Jr.
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  2. Jerry W. Shay
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  3. Leonard R. Murrell
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Crenshaw, A.H., Shay, J.W. & Murrell, L.R. Micronucleation of human somatic cells with colcemid. Journal of Tissue Culture Methods 8, 185–187 (1983). https://doi.org/10.1007/BF01665884

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  • DOI: https://doi.org/10.1007/BF01665884

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