Abstract
A new genetically polymorphic cell surface antigen recognized by a monoclonal rat anti-mouse antibody is expressed on mouse lymphoid cells. Fluorescence analysis on the fluorescence-activated cell sorter (FACS) locates the antigen on thymocytes, lymph node cells, and both T and B cells in the spleen. It also appears on approximately 40% of cells in the bone marrow.
Immune precipitations from surface iodinated spleen cells followed by 2-D gel electrophoresis demonstrate that the antigen is a glycoprotein of approximately 100,000 daltons. Since it is expressed in all lymphoid tissues and on both T and B cells, we designate it lymphoid glycoprotein 100 (Lgp100).
Strains with Lgp100 include A/J, AKR/J, AKR/Cu, BALB/c, 129/J, CBA/N, C3H/HeJ, CBA/2J, and SJL/J. Strains with no detectable antigen include C57BL/6J, C57BL/10J, C57BR/cdJ, C57L/J, and C58/J. Intercrosses and backcrosses establish a pair of alleles, a positive and a negative one, at a single locus. Heterozygotes display about 50% as much antigen as homozygotes by quantitative membrane immunofluorescence on the FACS. Tests for Lgp100 in 35 recombinant inbred strains from three crosses (CXB, BXB, and BXH) locate this locus on chromosome 1, closely linked to theMls locus.
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References
Bailey, D. W.: Recombinant-inbred strains; an aid to finding identity, linkage, and function of histocompatibility and other genes.Transplantation 11:325–327, 1971
Baur, S., Vitetta, E. S., Sherr, C. J., Schenkein, I., and Uhr, J. W.: Isolation of heavy and light chains of immunoglobulin from the surfaces of lymphoid cells.J. Immunol. 106:1133–1135, 1971
Bonner, W. A., Hulett, H. R., Sweet, R. G., and Herzenberg, L. A.: Fluorescence activated cell sorting.Rev. Sci. Instrum. 43:404–409, 1972
Durda, P. J., Boos, S. C., and Gottleib, P. D.: T100: A new murine cell surface glycoprotein detected by anti-Lyt-2.1 serum.J. Immunol. in press, 1979
Festenstein, H.: The Mls system.Transplant. Proc. 8:339–342, 1976
Festenstein, H., Abbasi, K., Sachs, J. A., and Oliver, R. T. D.: Serologically undetectable immune responses in transplantation.Transplant. Proc. 4:219–222, 1972
Festenstein, H., Bishop, C., and Taylor, B. A.: Location of Mls locus on mouse chromosome 1.Immunogenetics 5:357–361, 1977
Hämmerling, G. J., Lemke, H., Hämmerling, U., Höhmann, C., Wallich, R., and Rajewsky, K.: Monoclonal antibodies against murine cell surface antigens: Anti-H-2, anti-Ia, and anti-T cell antibodies.Curr. Top. Microbiol. Immunol. 81:100–106, 1978
Herzenberg, L. A. and Herzenberg, L. A.: Mouse immunoglobulin allotypes: Description and special methodology.In D. M. Weir (ed.):Handbook of Experimental Immunology, 3rd ed. Chapter 12,12.1–12.23, Blackwell Sci. Pub., Oxford, England, 1978a
Herzenberg, L. A. and Herzenberg, L. A.: Analysis and separation using the fluorescence activated cell sorter (FACS).In D. M. Weir (ed):Handbook of Experimental Immunology, 3rd ed., Chapter 22,22.1–22.21, Blackwell Sci. Pub., Oxford, England, 1978b
Julius, M. H., Simpson, E., and Herzenberg, L. A.: A rapid method for the isolation of functional thymus derived murine lymphocytes.Eur. J. Immunol. 3:645–649, 1973
Kessler, S. W.: Rapid isolation of antigens from cells with a staphylococcal protein A-antibody absorbant; parameters of the interaction of antigen-antibody complexes with protein A.J. Immunol. 115:1617–1622, 1975
Köhler, G., Howe, S. C., and Milstein, C.: Fusion between immunoglobulin-secreting and non-secreting myeloma cell lines.Eur. J. Immunol. 6:292–295, 1976
Köhler, G. and Milstein, C.: Continuous cultures of fused cells secreting antibody of predefined specificity.Nature 256:495–497, 1975
Ledbetter, J. A.: Two dimensional analysis of murine leukemia virusgag gene polyproteins.Virology, in press, 1979
Loken, M. R., and Herzenberg, L. A.: Analysis of cell populations with a flourescence-activated cell sorter.Annals N. Y. Acad. Sci. 254:163–171, 1975
Loken, M. R., Parks, D. R., and Herzenberg, L. A.: Two color immunofluorescence using a fluorescence activated cell sorter.J. Histochem. Cytochem. 24:899–907, 1977
McCann, J., Choi, E., Yamasaki, E., and Ames, B. N.: Detection of carcinogens as mutagens in the salmonella/microsome test: Assay of 300 chemicals.Proc. Natl. Acad. Sci. U.S.A. 72:5135–5139, 1975
Morse, H. C. III. Historical perspective on the development of inbred mice.In H. C. Morse III (ed.)Origins of Inbred Mice, pp. 3–21, Academic Press, N.Y.
Murphy, D. B. and Schreffler, D. C.: Cross-reactivity between H-2 K and H-2 D products. I. Evidence for extensive and reciprocal serological cross-reactivity.J. Exp. Med. 141:347–391, 1975
O'Farrell, P. Z., Goodman, H. M., and O'Farrell, P. H.: High resolution two-dimensional electrophoresis of basic as well as acidic proteins.Cell 12:1133–1142, 1977
Oi, V. T. and Herzenberg, L. A.: Immunoglobulin-producing hybrid cell lines.In B. B. Mishell and S. M. Shiigi (eds.):Selected Methods in Cellular Immunology, Chap. 17, W. H. Freeman, San Francisco, in press
Oi, V. T., Jones, P. P., Goding, J. W., Herzenberg, L. A., and Herzenberg, L. A.: Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens.Curr. Top. Microbiol. Immunol. 81:115–129, 1978
Parks, D. R., Bryan, V. M., Oi, V. T., and Herzenberg, L. A.: Antigen specific identification and cloning of hybridomas with a fluorescence-activated cell sorter (FACS).Proc. Natl. Acad. Sci. U.S.A. 76:1962–1966, 1979
Pearson, T., Galfré, G., Ziegler, A., and Milstein, C.: A myeloma hybrid producing antibody specific for an allotypic determinant on “IgD-like” molecules of the mouse.Eur. J. Immunol. 7:684–690, 1977
Sachs, J. A., Huber, B., Pena-Martinez, J., and Festenstein, H.: Genetic studies and effect on skin allograft survival of DBA/2, DAG, Ly and M-locus antigens.Transpl. Proc.5:1385–1387, 1973
Springer, T., Galfré, G., Secher, D., and Milstein, C.: Monoclonal xenogenic antibodies to mouse leukocyte antigens: Identification of macrophage-specific and other differentiation antigens.Curr. Top. Microbiol. Immunol. 81:45–50, 1978a
Springer, T., Galfré, G., Secher, D. S., and Milstein, C.: Monoclonal xenogenic antibodies to murine cell surface antigens: Identification of novel leukocyte differentiation antigens.Eur. J. Immunol. 8:539–551, 1978b
Taylor, B. A., Bailey, D. W., Cherry, M., Riblet, R., and Weigert, M.: Genes for immunoglobulin heavy chain and serum prealbumin protein are linked in the mouse.Nature 256:644–646, 1975
Taylor, B. A.: Recombinant inbred strains: Use in gene mapping.In H. C. Morse, III (ed.):Origins of Inbred Mice, pp. 423–428, Academic Press, New York, 1978
Tonkonogy, S. L. and Winn, H. J.: A new alloantigenic system associated with the Mls locus in the mouse.J. Immunol. 116:835–846, 1976
Trowbridge, I. S. and Mazauskas, C.: Immunological properties of murine thymus-dependent surface glycoproteins.Eur. J. Immunol. 6:557–562, 1976
Trowbridge, I. S.: Interspecies spleen-myeloma hybrid producing monoclonal antibodies against the mouse lymphocyte surface glycoprotein, T200.J. Exp. Med. 148:313–323, 1978
Tsu, T. T. and Herzenberg, L. A.: Solid-phase radioimmune assays.In B. B. Mishell and S. M. Shiigi (eds.):Selected Methods in Cellular Immunology, Chapter 18, W. H. Freeman, San Francisco (in press)
Williams, A. F., Giovanni, G. and Milstein, C.: Analysis of cell surface by xenogenic myeloma hybrid antibodies: differentiation antigens of rat lymphocytes.Cell 12:663–673, 1977
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Ledbetter, J.A., Goding, J.W., Tsu, T.T. et al. A new mouse lymphoid alloantigen (Lgp100) recognized by a monoclonal rat antibody. Immunogenetics 8, 347–360 (1979). https://doi.org/10.1007/BF01561445
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DOI: https://doi.org/10.1007/BF01561445